Now showing items 1-20 of 20

    • Apyrase activity and changes in metabolites during germination and tuberization of Solanum tuberosum 

      Anich, Mario; Fanta, Nora; Mancilla, Marta; Kettlun, Ana María; Valenzuela, María Antonieta; Traverso Cori, A. (1990)
      Levels of adenine nucleotides, inorganic phosphate, starch and apyrase were measured during the growth of Solanum tuberosum tuber. The data obtained from potatoes sown in summer were compared with the data already reported ...
    • ATP fosfonato y azul de cibacron unidos a poliextrano para purificar pirofosfohidrolasas 

      Calvo Pérez, Víctor; Valenzuela Pedevila, María Antonieta; Traverso Cori, A. (Centro de Perfeccionamiento, Experimentación e Investigaciones Pedagógicas (Chile), 1979)
      No tiene
    • ATP-diphosphohydrolase activity in rat renal microvillar membranes and vascular tissue 

      Sandoval, S.; García Nannig, Lorena; Mancilla, Marta; Kettlun, Ana María; Collados, L. E.; Chayet, L.; Alvarez, A.; Traverso Cori, A.; Valenzuela Pedevila, María Antonieta (PERGAMON-ELSEVIER SCIENCE LTD, 1996-05)
      Ecto-nucleotidases may have a role in the regulation of purinoceptor-mediated responses. ATP-diphosphohydrolase or apyrase has been described as an ecto-nucleotidase, which is characterized by a low specificity for its ...
    • ATP-diphosphophydrolase activin in rat heart tissue 

      Espinosa, Victoria; Galleguillos, M.; Mancilla, Marta; Garrido, J.; Kettlun, Ana María; Collados, L. E.; Chayet, L.; García Nannig, Lorena; Traverso Cori, A.; Valenzuela Pedevila, María Antonieta (ACADEMIC PRESS AUST, 1996-08)
      Extracellular nucleotides interact with specific receptors on the cell surface and are locally metabolized by ecto-nucleotidases. Biochemical characterization of the ATPase and ADPase activities detected in rat heart ...
    • ATPASE-ADPASE ACTIVITIES OF RAT PLACENTAL TISSUE 

      Pieber, M.; Valenzuela Pedevila, María Antonieta; Kettlun, Ana María; Mancilla, Marta; Aranda, E.; Collados, L. E.; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1991)
      1. Calcium-stimulated ATPase-ADPase activities were studied in a microsomal fraction of rat placental tissue. 2. The kinetic characteristics correspond to those of ATP-diphosphohydrolase, also known as apyrase (E.C. ...
    • Changes in apyrase activity in uterus and mammary gland during the lactogenic cycle 

      Valenzuela, M. A.; Collados, L.; Kettlun, A. M.; Mancilla, M.; Puente, J.; Aranda, E.; Chayet, L.; Alvarez, A.; Traverso Cori, A. (1992)
      1. 1. The purpose of this present research was to explore the possible roles of ATP-diphosphohydrolase (apyrase) in two tissues with high energetic demands during cell proliferation and differentiation. 2. 2. Changes in ...
    • Characterization of ATP-diphosphohydrolase from rat mammary gland 

      Alvarez, A.; Chayet, L.; Galleguillos, M.; García Nannig, Lorena; Kettlun, Ana María; Collados, L. E.; Traverso Cori, A.; Mancilla, M.; Valenzuela Pedevila, María Antonieta (PERGAMON-ELSEVIER SCIENCE LTD, 1996-01)
      ATP-diphosphohydrolase (or apyrase) hydrolyses nucleoside di- and triphosphates in the presence of millimolar concentration of divalent cations. It is insensitive towards sulfhydryl and aliphatic hydroxyl-selective reagents ...
    • Comparative subcellular distribution of apyrase from animal and plant sources. Characterization of microsomal apyrase 

      Valenzuela,; López,; Depix,; Mancilla,; Kettlun,; Catalán,; Chiong Lay, Mario; Garrido,; Traverso Cori, A. (1989)
      1. 1. Apyrase (ATP: diphosphohydrolase) has been found in the microsomal fraction of rat salivary gland, mammary gland and uterus. 2. 2. This enzyme, already described in plant tissues, is mainly present as a soluble ...
    • Comparison of the biochemical properties, regulation and function of ATP-diphosphohydrolase from human placenta and rat kidney 

      Valenzuela Pedevila, María Antonieta; Kettlun, Ana María; Sandoval, S.; García Nannig, Lorena; Mancilla, Marta; Neckelmann, G.; Chayet, L.; Alvarez, A.; Cuevas, F.; Collados, L. E.; Espinosa, Victoria; Traverso Cori, A.; Bravo, I.; Acevedo, C. G.; Aranda, E. (ASSOC BRAS DIVULG CIENTIFICA, 1996-05)
      ATP-diphosphohydrolase (apyrase, EC 3.6.1.5) has both ATPase and ADPase activity that are stimulated by bivalent metals, with Ca2+ being the most effective. The possible physiological function of this enzyme, associated ...
    • HUMAN PLACENTAL ATP-DIPHOSPHOHYDROLASE - BIOCHEMICAL .CHARACTERIZATION, REGULATION AND FUNCTION 

      Kettlun, Ana María; Alvarez, A.; Quintar, R.; Valenzuela Pedevila, María Antonieta; Collados, L. E.; Aranda, E.; Banda, A.; Chayet, L.; Chiong Lay, Mario; Mancilla, Marta; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1994-03)
      1. Kinetic and physico-chemical studies on human placental microsomal fraction confirmed that the ATPase and ADPase activities detected in this fraction correspond to the enzyme ATP-diphosphohydrolase or apyrase (EC 3.6.1.5). ...
    • Hydrolysis of synthetic pyrophosphoric esters by an isoenzyme of apyrase from Solanum tuberosum 

      Del Campo, Guillermo; Puente, Javier; Valenzuela, M. Antonieta; Traverso Cori, A.; Cori, Osvaldo (1977)
      A highly purified isoenzyme of apyrase obtained from potatoes (Solanum tuberosum var. Pimpernel) exhibits a low specificity for the organic moiety of synthetic pyro- and triphosphates. Methyl di- and tri-phosphates were ...
    • Identification and subcellular localization of two isoenzymes of apyrase from Solanum tuberosum 

      Kettlun, Ana María; Leyton, Mario; Valenzuela, María Antonieta; Mancilla, Marta; Traverso Cori, A. (1992)
      Two forms of ATP-diphosphohydrolase were identified in Solanum tuberosum tuber var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were over 10 for form A and 1 for form B. In the potato tuber homogenate the ...
    • IDENTIFICATION AND SUBCELLULAR-LOCALIZATION OF 2 ISOENZYMES OF APYRASE FROM SOLANUM-TUBEROSUM 

      Kettlun, Ana María; Leyton, Mario; Valenzuela Pedevila, María Antonieta; Mancilla, Marta; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1992-06)
      Two forms of ATP-diphosphohydrolase were identified in Solanum tuberosum tuber var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were over 10 for form A and 1 for form B. In the potato tuber homogenate the ...
    • Identification of an apyrase activating protein and of calmodulin in Solanum tuberosum 

      Mancilla, Marta; Valenzuela, Marta; Anich, Mario; Kettlun, Ana María; Jara, Otilia; Traverso Cori, A. (1987)
      Three types of effector proteins have been isolated from a partially purified protein preparation of potato tuber. One of the proteins is a typical calmodulin which has no effect on apyrase. The two other proteins modulate ...
    • Kinetic studies and properties of potato apyrase 

      Traverso Cori, A.; Chaimovich, Aida; Cori, Aida (1965)
      Apyrase from potato extracts has been purified 200-fold by two different procedures; both procedures furnish an apyrase which splits ATP faster than ADP. By using P32-labeled substrates, it was possible to demonstrate that ...
    • Properties of two apyrases from Solanum tuberosum 

      Kettlun, Ana M.; Uribe, Luz; Calvo, Luz; Silva, Luz; Rivera, Luz; Mancilla, Marta; Antonieta, Marta; Valenzuela, Marta; Traverso Cori, A. (1982)
      Two homogeneous isoenzymes of apyrase from Pimpernel and Desirée varieties of Solanum tuberosum were obtained by affinity chromatography on agarose-Cibacron Blue or agarose-ATP-phosphonate columns. Both enzymes split POP ...
    • PURIFICATION AND CHARACTERIZATION OF 2 ISOAPYRASES FROM SOLANUM-TUBEROSUM VAR ULTIMUS 

      Kettlun, Ana María; Urra, Raúl; Leyton, Mario; Valenzuela Pedevila, María Antonieta; Mancilla, Marta; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1992-11)
      Two isoenzymes of ATP-diphosphohydrolase (apyrase) were extracted and purified from S. tuberosum var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were 1.0 (apyrase B) and ca 15.0 (apyrase A). They were ...
    • Purification and characterization of two isoapyrases from Solanum tuberosum var. ultimus 

      Kettlun, Ana María; Urra, Raúl; Leyton, Mario; Valenzuela, M.Antonieta; Mancilla, Marta; Traverso Cori, A. (1992)
      Two isoenzymes of ATP-diphosphohydrolase (apyrase) were extracted and purified from S. tuberosum var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were 1.0 (apyrase B) and ca 15.0 (apyrase A). They were ...
    • Síntesis de análogos de ATP unidos a Sepharosa en la purificación de una pirofosfohidrolasa. 

      Calvo Pérez, Víctor; Valenzuela Pedevila, María Antonieta; Traverso Cori, A. (Centro de Perfeccionamiento, Experimentación e Investigaciones Pedagógicas (Chile), 1979)
      No tiene
    • The effect of bivalent metal ions on ATPase-ADPase activities of apyrase from Solanum tuberosum 

      Valenzuela, María Antonieta; Kettlun, Ana María; Mancilla, Marta; Calvo, Victor; Fanta, Nora; Traverso Cori, A. (1988)
      The purpose of this study was the elucidation of the possible importance of bivalent metal ions in controlling the activity of apyrase (ATP: diphosphohydrolase EC 3.6.1.5) purified from tubers of Solanum tuberosum cv ...