Now showing items 1-14 of 14

    • Aislamiento de bacterias termofílicas productoras de xilanasas 

      Steiner W., Jeannette; Mella C., Marcos; Polanco, R.; Ortega A., Ximena; Eyzaguirre, Jaime (Asociación Chilena de Microbiología, 1996)
      Las xilanasas son enzimas, producidas por hongos y bacterias que degradan el xilano de las hemicelulosas vegetales y tienen una importante aplicación en el bioblanqueamiento de la celulosa. A partir de compost, se ...
    • BETA-GLUCOSIDASE FROM PENICILLIUM-PURPUROGENUM - PURIFICATION AND PROPERTIES 

      Hidalgo, Mauricio; Steiner, Jeannette; Eyzaguirre, Jaime (PORTLAND PRESS, 1992-04)
      Beta-glucosidade was purified from the culture supernatant of penicillium purpurogenum. The purified enzyme was homogeneous on both nondenaturing and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The ...
    • Cloning, sequencing and expression of the cDNA of endoxylanase B from Penicillium purpurogenum 

      Díaz, René; Sapag, Amalia; Peirano, Alessandra; Steiner, Jeannette; Eyzaguirre, Jaime (ELSEVIER SCIENCE BV, 1997-03-18)
      The cDNA, for xylanase B from Penicillium purpurogenum was cloned and sequenced. This DNA encodes a protein of 208 amino acids which is expected to yield a protein of 183 residues upon processing of the N terminus. The ...
    • Cold-active xylanase produced by fungi associated with Antarctic marine sponges 

      Del-Cid, Abdiel; Ubilla, Pamela; Ravanal, María Cristina; Medina, Exequiel; Vaca Cerezo, Inmaculada; Levicán, Gloria; Eyzaguirre, Jaime; Chávez, Renato (2014)
      Despite their potential biotechnological applications, cold-active xylanolytic enzymes have been poorly studied. In this work, 38 fungi isolated from marine sponges collected in King George Island, Antarctica, were screened ...
    • CULTURE CONDITIONS FOR ENHANCED CELLULASE PRODUCTION BY A NATIVE STRAIN OF PENICILLIUM-PURPUROGENUM 

      Steiner, Jeannette; Socha, C.; Eyzaguirre, Jaime (RAPID SCIENCE PUBLISHERS, 1994-05)
      A cellulolytic wild-type strain of Penicillium purpurogenum was isolated from a soil sample in southern Chile. It grew best at 28-degrees-C from an inoculum of 4 x 10(7) spores/100 ml medium. Highest endoglucanase activity ...
    • Evidence of essential arginyl residues in rabbit muscle pyruvate kinase 

      Cardemil, Emilio; Eyzaguirre, Jaime (1979)
      Rabbit muscle pyruvate kinase is inactivated by 2,3-butanedione in borate buffer. The inactivation follows pseudo-first-order kinetics with a calculated second-order rate constant of 4.6 m-1 min-1. The modification can be ...
    • Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans 

      Ravanal, María Cristina; Espinosa, Yeison; Rosa, Lorena; Vaca Cerezo, Inmaculada; Polanco, Rubén; Eyzaguirre, Jaime; Levicán, Gloria; Chávez, Renato (Springer, 2012)
      The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own ...
    • PENICILLIUM-PURPUROGENUM PRODUCES SEVERAL XYLANASES - PURIFICATION AND PROPERTIES OF 2 OF THE ENZYMES 

      Belancic, Andrea; Scarpa, Juan; Peirano, Alessandra; Díaz, René; Steiner, Jeannette; Eyzaguirre, Jaime (ELSEVIER SCIENCE BV, 1995-07-15)
      The fungus Penicillium purpurogenum produces several extracellular xylanases. The two major forms (xylanases A and B) have been purified and characterized. After ammonium sulfate precipitation and chromatography in Bio-Gel ...
    • Pig Liver Phosphomevalonate Kinase. 1. Purification and Properties 

      Bazaes, Sergio; Beytía, Enrique; de Ovando, Francisco Solís; Lagos Gómez, Isabel; Eyzaguirre, Jaime; Jabalquinto, Ana María (1980)
      Pig liver phosphomevalonate kinase (EC 2.7.4.2) has been purified to homogeneity as shown by polyacrylamide gel electrophoresis. The molecular weight estimates range from 21000 to 22500. Each molecule is composed of one ...
    • Pig Liver Phosphomevalonate Kinase. 2. Participation of Cysteinyl and Lysyl Groups in Catalysis 

      Bazaes, Sergio; Beytía, Enrique; de Ovando, Francisco Solís; Lagos Gómez, Isabel; Eyzaguirre, Jaime; Jabalquinto, Ana María (1980)
      Phosphomevalonate kinase from pig liver is inactivated by 5,5'-dithiobis(2-nitrobenzoate) and pyridoxal 5'-phosphate. The substrate phosphomevalonate protects the enzyme against inactivation by these reagents. Inactivation ...
    • Purification and Characterization of Avian Liver Mevalonate-5-pyrophosphate Decarboxylase 

      Alvear, Marysol; Jabalquinto, Ana María; Eyzaguirre, Jaime; Cardemil, Emilio (1982)
      Mevalonate-5-pyrophosphate decarboxylase [ATP:5-diphosphomevalonate car boxy-lyase (dehydrating), EC 4.1.1.33] has been purified 5800 times from chicken liver and obtained in a stable and highly purified form. The protein ...
    • Short communication: Isolation of mutants of Penicillium purpurogenum with enhanced xylanase and beta-xylosidase production 

      Steiner, Jeannette; Carmona, P.; Ponce, C.; Berti, M.; Eyzaguirre, Jaime (KLUWER ACADEMIC PUBL, 1998-07)
      Penicillium purpurogenum was mutated with u.v. light to increase xylanase production. The best mutant, UV-64, was treated with N-methyl-N'-nitro-N-nitrosoguanidine and a second generation of mutants was obtained (NG-188 ...
    • The endoxylanases from family 11: computer analysis of protein sequences reveals important structural and phylogenetic relationships 

      Sapag, Amalia; Wouters, Johan; Lambert, Christophe; Ioannes, Pablo de; Eyzaguirre, Jaime; Depiereux, Eric (ELSEVIER SCIENCE BV, 2002-05-09)
      Eighty-two amino acid sequences of the catalytic domains of mature endoxylanases belonging to family 11 have been aligned using the programs MATCHBOX and CLUSTAL. The sequences range in length from 175 to 233 residues. The ...
    • Two malic enzymes in Pseudomonas aeruginosa 

      Eyzaguirre, Jaime; Cornwell, E.; Borie B., Gilda; Ramírez, B. (American Society of Microbiology, 1973)
      Cell-free extract supernatant fluids of Pseudomonas aeruginosa were shown to lack malic dehydrogenase but possess a nicotinamide adenine dinucleotide (NAD) - or NAD phosphate (NADP)-dependent enzymatic activity, with ...