Now showing items 1-15 of 15

    • Apyrase activity and changes in metabolites during germination and tuberization of Solanum tuberosum 

      Anich, Mario; Fanta, Nora; Mancilla, Marta; Kettlun, Ana María; Valenzuela, María Antonieta; Traverso Cori, A. (1990)
      Levels of adenine nucleotides, inorganic phosphate, starch and apyrase were measured during the growth of Solanum tuberosum tuber. The data obtained from potatoes sown in summer were compared with the data already reported ...
    • ATP-diphosphohydrolase activity in rat renal microvillar membranes and vascular tissue 

      Sandoval, S.; García Nannig, Lorena; Mancilla, Marta; Kettlun, Ana María; Collados, L. E.; Chayet, L.; Alvarez, A.; Traverso Cori, A.; Valenzuela Pedevila, María Antonieta (PERGAMON-ELSEVIER SCIENCE LTD, 1996-05)
      Ecto-nucleotidases may have a role in the regulation of purinoceptor-mediated responses. ATP-diphosphohydrolase or apyrase has been described as an ecto-nucleotidase, which is characterized by a low specificity for its ...
    • ATP-diphosphophydrolase activin in rat heart tissue 

      Espinosa, Victoria; Galleguillos, M.; Mancilla, Marta; Garrido, J.; Kettlun, Ana María; Collados, L. E.; Chayet, L.; García Nannig, Lorena; Traverso Cori, A.; Valenzuela Pedevila, María Antonieta (ACADEMIC PRESS AUST, 1996-08)
      Extracellular nucleotides interact with specific receptors on the cell surface and are locally metabolized by ecto-nucleotidases. Biochemical characterization of the ATPase and ADPase activities detected in rat heart ...
    • ATPASE-ADPASE ACTIVITIES OF RAT PLACENTAL TISSUE 

      Pieber, M.; Valenzuela Pedevila, María Antonieta; Kettlun, Ana María; Mancilla, Marta; Aranda, E.; Collados, L. E.; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1991)
      1. Calcium-stimulated ATPase-ADPase activities were studied in a microsomal fraction of rat placental tissue. 2. The kinetic characteristics correspond to those of ATP-diphosphohydrolase, also known as apyrase (E.C. ...
    • Comparison of the biochemical properties, regulation and function of ATP-diphosphohydrolase from human placenta and rat kidney 

      Valenzuela Pedevila, María Antonieta; Kettlun, Ana María; Sandoval, S.; García Nannig, Lorena; Mancilla, Marta; Neckelmann, G.; Chayet, L.; Alvarez, A.; Cuevas, F.; Collados, L. E.; Espinosa, Victoria; Traverso Cori, A.; Bravo, I.; Acevedo, C. G.; Aranda, E. (ASSOC BRAS DIVULG CIENTIFICA, 1996-05)
      ATP-diphosphohydrolase (apyrase, EC 3.6.1.5) has both ATPase and ADPase activity that are stimulated by bivalent metals, with Ca2+ being the most effective. The possible physiological function of this enzyme, associated ...
    • HUMAN PLACENTAL ATP-DIPHOSPHOHYDROLASE - BIOCHEMICAL .CHARACTERIZATION, REGULATION AND FUNCTION 

      Kettlun, Ana María; Alvarez, A.; Quintar, R.; Valenzuela Pedevila, María Antonieta; Collados, L. E.; Aranda, E.; Banda, A.; Chayet, L.; Chiong Lay, Mario; Mancilla, Marta; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1994-03)
      1. Kinetic and physico-chemical studies on human placental microsomal fraction confirmed that the ATPase and ADPase activities detected in this fraction correspond to the enzyme ATP-diphosphohydrolase or apyrase (EC 3.6.1.5). ...
    • Identification and subcellular localization of two isoenzymes of apyrase from Solanum tuberosum 

      Kettlun, Ana María; Leyton, Mario; Valenzuela, María Antonieta; Mancilla, Marta; Traverso Cori, A. (1992)
      Two forms of ATP-diphosphohydrolase were identified in Solanum tuberosum tuber var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were over 10 for form A and 1 for form B. In the potato tuber homogenate the ...
    • IDENTIFICATION AND SUBCELLULAR-LOCALIZATION OF 2 ISOENZYMES OF APYRASE FROM SOLANUM-TUBEROSUM 

      Kettlun, Ana María; Leyton, Mario; Valenzuela Pedevila, María Antonieta; Mancilla, Marta; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1992-06)
      Two forms of ATP-diphosphohydrolase were identified in Solanum tuberosum tuber var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were over 10 for form A and 1 for form B. In the potato tuber homogenate the ...
    • Identification of an apyrase activating protein and of calmodulin in Solanum tuberosum 

      Mancilla, Marta; Valenzuela, Marta; Anich, Mario; Kettlun, Ana María; Jara, Otilia; Traverso Cori, A. (1987)
      Three types of effector proteins have been isolated from a partially purified protein preparation of potato tuber. One of the proteins is a typical calmodulin which has no effect on apyrase. The two other proteins modulate ...
    • Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni - Immunological cross-reactivities with potato apyrase and Toxoplasma gondii nucleoside triphosphate hydrolase 

      Vasconcelos, E. G.; Ferreira, Sergio T.; Técia, M. U.; De Souza, Wanderley; Kettlun, Ana María; Mancilla, Marta; Valenzuela Pedevila, María Antonieta; Verjovski Almeida, S. (AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 1996-09-06)
      ATP diphosphohydrolase from tegumental membranes of Schistosoma mansoni was solubilized with Triton X-100 plus deoxycholate and separated by preparative nondenaturing polyacrylamide gel electrophoresis. Two isoforms with ...
    • Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni: Immunological cross-reactivities with potato apyrase and Toxoplasma gondii nucleoside triphosphate hydrolase 

      Vasconcelos, Eveline G.; Ferreira, Sérgio T.; De Carvalho, Técia M U; De Souza, Wanderley; Kettlun, Ana M.; Mancilla, Marta; Valenzuela, M. Antonieta; Verjovski-Almeida, Sergio (1996)
      ATP diphosphohydrolase from tegumental membranes of Schistosoma mansoni was solubilized with Triton X-100 plus deoxycholate and separated by preparative nondenaturing polyacrylamide gel electrophoresis. Two isoforms with ...
    • Properties of two apyrases from Solanum tuberosum 

      Kettlun, Ana M.; Uribe, Luz; Calvo, Luz; Silva, Luz; Rivera, Luz; Mancilla, Marta; Antonieta, Marta; Valenzuela, Marta; Traverso Cori, A. (1982)
      Two homogeneous isoenzymes of apyrase from Pimpernel and Desirée varieties of Solanum tuberosum were obtained by affinity chromatography on agarose-Cibacron Blue or agarose-ATP-phosphonate columns. Both enzymes split POP ...
    • PURIFICATION AND CHARACTERIZATION OF 2 ISOAPYRASES FROM SOLANUM-TUBEROSUM VAR ULTIMUS 

      Kettlun, Ana María; Urra, Raúl; Leyton, Mario; Valenzuela Pedevila, María Antonieta; Mancilla, Marta; Traverso Cori, A. (PERGAMON-ELSEVIER SCIENCE LTD, 1992-11)
      Two isoenzymes of ATP-diphosphohydrolase (apyrase) were extracted and purified from S. tuberosum var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were 1.0 (apyrase B) and ca 15.0 (apyrase A). They were ...
    • Purification and characterization of two isoapyrases from Solanum tuberosum var. ultimus 

      Kettlun, Ana María; Urra, Raúl; Leyton, Mario; Valenzuela, M.Antonieta; Mancilla, Marta; Traverso Cori, A. (1992)
      Two isoenzymes of ATP-diphosphohydrolase (apyrase) were extracted and purified from S. tuberosum var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were 1.0 (apyrase B) and ca 15.0 (apyrase A). They were ...
    • The effect of bivalent metal ions on ATPase-ADPase activities of apyrase from Solanum tuberosum 

      Valenzuela, María Antonieta; Kettlun, Ana María; Mancilla, Marta; Calvo, Victor; Fanta, Nora; Traverso Cori, A. (1988)
      The purpose of this study was the elucidation of the possible importance of bivalent metal ions in controlling the activity of apyrase (ATP: diphosphohydrolase EC 3.6.1.5) purified from tubers of Solanum tuberosum cv ...