Browsing by Author "e805ca02-a02c-4352-b75a-bac912189124"

Now showing items 1-7 of 7

    • ADP-dependent kinases from the archaeal order methanosarcinales adapt to salt by a non-canonical evolutionarily ... 
      González Órdenes, Felipe; Cea, Pablo A.; Fuentes, Nicolás; Muñoz, Sebastián M.; Zamora, Ricardo A.; Leonardo, Diego; Garratt, Richard C.; Castro Fernández, Víctor; Guixé Leguía, Victoria Cristina (Frontiers Media SA., 2018-06-26)
      Halophilic organisms inhabit hypersaline environments where the extreme ionic conditions and osmotic pressure have driven the evolution of molecular adaptation mechanisms. Understanding such mechanisms is limited by the ...
    • ADP-dependent phosphofructokinases from the archaeal order Methanosarcinales display redundant glucokinase activity 
      Zamora, Ricardo A.; González Órdenes, Felipe; Castro Fernández, Víctor; Guixé Leguía, Victoria Cristina (Academic Press Inc., 2017)
      © 2017 Elsevier Inc. The genome of Methanosarcinales organisms presents both ADP-dependent glucokinase and phosphofructokinase genes. However, Methanococcoides burtonii has a truncate glucokinase gene with a large deletion ...
    • Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes 
      Santiago, Margarita; Ramírez Sarmiento, Cesara; Zamora, Ricardo A.; Parra, Loreto P. (Frontiers Media, 2016)
      Cold-active enzymes constitute an attractive resource for biotechnological applications. Their high catalytic activity at temperatures below 25 degrees C makes them excellent biocatalysts that eliminate the need of heating ...
    • Evolution, metabolism and molecular mechanisms underlying extreme adaptation of euryarchaeota and its ... 
      Castro Fernández, Víctor; Zamora, Ricardo; Herrera Morandé, Alejandra; Vallejos, Gabriel; González Órdenes, Felipe; Guixé Leguía, Victoria Cristina (IntechOpen, 2017)
      Archaeal organisms harbor many unique genotypic and phenotypic properties, testifying their peculiar evolutionary status. Thus, the so‐called extremophiles must be adequately adapted to cope with many extreme environments ...
    • Free radicals derived from γ-radiolysis of water and AAPH thermolysis mediate oxidative crosslinking of ... 
      Zamora, Ricardo A.; Fuentes Lemus, Eduardo; Barrias, Pablo; Herrera Morandé, Alejandra; Mura, Francisco; Guixé, Victoria; Castro Fernández, Víctor; Rojas, Tomás; López Alarcón, Camilo; Aguirre, Paulina; Rivas Aravena, Andrea; Aspée, Alexis (Elsevier, 2020)
      The enhanced green fluorescent protein (eGFP) is one of the most employed variants of fluorescent proteins. Nonetheless little is known about the oxidative modifications that this protein can undergo in the cellular ...
    • The folding unit of phosphofructokinase-2 as defined by the biophysical properties of a monomeric mutant 
      Ramírez Sarmiento, César A.; Báez, Mauricio; Zamora, Ricardo A.; Balasubramaniam, Deepa; Babul Cattán, Jorge; Komives, Elizabeth; Guixé Leguía, Victoria Cristina (Biophysical Society, 2015)
      © 2015 Biophysical Society. Escherichia coli phosphofructokinase-2 (Pfk-2) is an obligate homodimer that follows a highly cooperative three-state folding mechanism N<inf>2</inf> → 2I → 2U. The strong coupling ...
    • Tuning of conformational dynamics through evolution-based design modulates the catalytic adaptability of ... 
      Zamora, Ricardo A.; Ramírez Sarmiento, César; Castro Fernández, Víctor; Villalobos, Pablo; Maturana, Pablo; Herrera Morandé, Alejandra; Komives, Elizabeth A.; Guixe, Victoria (American Chemical Society, 2020)
      Conformational flexibility plays a critical role in enzyme function and is a key aspect in transitions from an open to a closed state induced by substrate binding and product release. Psychrophilic enzymes display a high ...