Browsing by Author "b5739206-8177-4df1-ab97-b9f834c494ac"
Now showing items 21-27 of 27
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Castro Fernández, Víctor; Herrera Morande, Alejandra; Zamorano Sarria, Ricardo; Merino, Felipe; González Órdenes, Felipe; Padilla Salinas, Felipe; Pereira, Humberto M.; Brandao Neto, José; Garratt, Richard C.; Guixé Leguía, Victoria Cristina (American Society for Biochemistry and Molecular Biology, 2017)One central goal in molecular evolution is to pinpoint the mechanisms and evolutionary forces that cause an enzyme to change its substrate specificity; however, these processes remain largely unexplored. Using the glycolytic ...
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Guixé Leguía, Victoria Cristina; Preller, Ana; Kessi, Eduardo; Ureta, Tito (Academic Press Inc., 1997)Glycogen synthesis following glucose microinjection in frog oocytes proceeds preferentially by an indirect pathway involving gluconeogenesis from triose compounds. Because of the known regulatory role of fructose-2,6-bisP ...
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Cabrera Paucar, Ricardo; Babul Cattán, Jorge; Guixé Leguía, Victoria Cristina (ELSEVIER SCIENCE INC, 2010-06-25)Phosphofructokinase-2 (Pfk-2) belongs to the ribokinase family and catalyzes the ATP-dependent phosphorylation of fructose-6-phosphate, showing allosteric inhibition by a second ATP molecule. Several structures have been ...
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Merino, Felipe; Guixé Leguía, Victoria Cristina (2008)In several archaea of the Euryarchaeota, the glycolytic flux proceeds through a modified version of the Embden-Meyerhof pathway, where the phosphofructokinase and glucokinase enzymes use ADP as the phosphoryl donor. These ...
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Acuña, Mariana; Castro Fernández, Víctor; Latorre Mora, Mauricio; Castro, Juan; Schuchman, Edward H.; Guixé Leguía, Victoria Cristina; González Canales, Mauricio; Zanlungo, Silvana (Elsevier, 2016)Niemann-Pick disease (NPD) type A and B are recessive hereditary disorders caused by deficiency in acid sphingomyelinase (ASM). The p.A1a359Asp mutation has been described in several patients but its functional and structural ...
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Báez, Mauricio; Rodríguez, Patricio H.; Babul Cattán, Jorge; Guixé Leguía, Victoria Cristina (2003)Modification of Escherichia coli phosphofructokinase-2 (Pfk-2) with pyrene maleimide (PM) results in a rapid inactivation of the enzyme. The loss of enzyme activity correlates with the incorporation of 2 mol of PM/mol of ...
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Ramírez Sarmiento, César A.; Báez, Mauricio; Zamora, Ricardo A.; Balasubramaniam, Deepa; Babul Cattán, Jorge; Komives, Elizabeth; Guixé Leguía, Victoria Cristina (Biophysical Society, 2015)© 2015 Biophysical Society. Escherichia coli phosphofructokinase-2 (Pfk-2) is an obligate homodimer that follows a highly cooperative three-state folding mechanism N<inf>2</inf> → 2I → 2U. The strong coupling ...