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IDENTIFICATION AND SUBCELLULAR-LOCALIZATION OF 2 ISOENZYMES OF APYRASE FROM SOLANUM-TUBEROSUM

Authordc.contributor.authorKettlun, Ana María 
Authordc.contributor.authorLeyton, Mario es_CL
Authordc.contributor.authorValenzuela Pedevila, María Antonieta es_CL
Authordc.contributor.authorMancilla, Marta es_CL
Authordc.contributor.authorTraverso Cori, A. es_CL
Admission datedc.date.accessioned2011-06-08T16:11:32Z
Available datedc.date.available2011-06-08T16:11:32Z
Publication datedc.date.issued1992-06
Cita de ítemdc.identifier.citationPHYTOCHEMISTRY 31 (6): 1889-1894es_CL
Identifierdc.identifier.issn0031-9422
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/121257
General notedc.descriptionArtículo de publicación ISIes_CL
Abstractdc.description.abstractTwo forms of ATP-diphosphohydrolase were identified in Solanum tuberosum tuber var. Ultimus. Their hydrolytic activity ratios (ATPase/ADPase) were over 10 for form A and 1 for form B. In the potato tuber homogenate the hydrolytic activity ratio is 3.0, as a result of contributions of the two forms of apyrase. These two apyrases (A and B) were partially separated and the possibility that they are produced as an artifact by partial proteolysis or subunit aggregation was excluded. The subcellular localization of the Ultimus isoapyrases was studied by differential centrifugation. These enzymes are localized in distinct compartments. The high ratio enzyme (A) lies mainly in the soluble fraction, while the low ratio apyrase (B) is principally bound to membranes. The two isoapyrases differ greatly in their kinetic properties and pI, but only slightly in M(r). Both enzymes immunocross-react with antiapyrase Desiree, which is important for isoenzyme detection by the immunowestern blot. This is the first example of two isoenzymes of apyrase in the same variety of S. tuberosum.es_CL
Lenguagedc.language.isoenes_CL
Publisherdc.publisherPERGAMON-ELSEVIER SCIENCE LTDes_CL
Keywordsdc.subjectSOLANUM-TUBEROSUMes_CL
Títulodc.titleIDENTIFICATION AND SUBCELLULAR-LOCALIZATION OF 2 ISOENZYMES OF APYRASE FROM SOLANUM-TUBEROSUMes_CL
Document typedc.typeArtículo de revistaes_CL


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