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Authordc.contributor.authorAmweg, A. N. 
Authordc.contributor.authorSalvetti, N. R. es_CL
Authordc.contributor.authorStangaferro, M. L. es_CL
Authordc.contributor.authorParedes Vargas, Alfonso es_CL
Authordc.contributor.authorLara, H. H. es_CL
Authordc.contributor.authorRodríguez, F. M. es_CL
Authordc.contributor.authorOrtega, H. H. es_CL
Admission datedc.date.accessioned2014-01-14T13:42:11Z
Available datedc.date.available2014-01-14T13:42:11Z
Publication datedc.date.issued2013
Cita de ítemdc.identifier.citationDomestic Animal Endocrinology 45 (2013) 126–140en_US
Identifierdc.identifier.otherDOI: 10.1016/j.domaniend.2013.07.001
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/121754
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractCystic ovarian disease (COD) is an important cause of infertility in cattle, and ACTH has been involved in regulatory mechanisms related to ovarian function associated with ovulation, steroidogenesis, and luteal function. Here, we examined the localization of 11bhydroxysteroid dehydrogenase type 1 (11bHSD1) and 11bHSD2 proteins in the ovary of healthy cows and animals with spontaneous and ACTH-induced COD and the in vitro response of the follicular wall exposed to ACTH. After stimulation by ACTH, we documented changes in 11bHSD expression and cortisol secretion by the follicular wall of large antral and follicular cysts. Follicular cysts showed a higher constitutive expression of both enzymes, whereas ACTH induced an increase in 11bHSD1 in tertiary follicles and follicular cysts and a decrease in 11bHSD2 in follicular cysts. Moderate expression of 11bHSD1 was observed by immunohistochemistry in granulosa of control animals, with an increase (P < 0.05) from primary to secondary, tertiary, and atretic follicles. The level of immunostaining in theca interna was lower than that in granulosa. The expression of 11bHSD2 was lower in the granulosa of primary follicles than in that of secondary, tertiary, and atretic follicles and was lower in the theca interna than in the granulosa. In ACTH-induced and spontaneously occurring follicular cysts, differences from controls were observed only in the expression of 11bHSD1 in the granulosa, being higher (P < 0.05) than in tertiary follicles. These findings indicate that follicular cysts may be exposed to high local concentrations of active glucocorticoids and indicate a local role for cortisol in COD pathogenesis and in regulatory mechanisms of ovarian function.en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherElsevieren_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectCystic ovarian diseaseen_US
Títulodc.titleOvarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian diseaseen_US
Document typedc.typeArtículo de revista


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile