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Hepatogenic and neurogenic differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetuses

Authordc.contributor.authorDueñas, Fernando 
Authordc.contributor.authorBecerra Ivanovic, Víctor Ignacio es_CL
Authordc.contributor.authorCortés Araya, Yennifer Alejandra es_CL
Authordc.contributor.authorVidal Vilches, Sonia es_CL
Authordc.contributor.authorSáenz Iturriaga, Leonardo Enrique es_CL
Authordc.contributor.authorPalominos Mackenney, Jaime es_CL
Authordc.contributor.authorReyes Solovera, Mónica de los es_CL
Authordc.contributor.authorPeralta Troncoso, Óscar es_CL
Admission datedc.date.accessioned2014-10-16T19:52:27Z
Available datedc.date.available2014-10-16T19:52:27Z
Publication datedc.date.issued2014-07-10
Cita de ítemdc.identifier.citationBMC Veterinary Research 2014, 10:154en_US
Identifierdc.identifier.issn1746-6148
Identifierdc.identifier.otherDOI: 10.1186/1746-6148-10-154
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/122534
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractBackground: Mesenchymal stem cells (MSC) are multipotent progenitor cells characterized by their ability to both self-renew and differentiate into tissues of mesodermal origin. The plasticity or transdifferentiation potential of MSC is not limited to mesodermal derivatives, since under appropriate cell culture conditions and stimulation by bioactive factors, MSC have also been differentiated into endodermal (hepatocytes) and neuroectodermal (neurons) cells. The potential of MSC for hepatogenic and neurogenic differentiation has been well documented in different animal models; however, few reports are currently available on large animal models. In the present study we sought to characterize the hepatogenic and neurogenic differentiation and multipotent potential of bovine MSC (bMSC) isolated from bone marrow (BM) of abattoir-derived fetuses. Results: Plastic-adherent bMSC isolated from fetal BM maintained a fibroblast-like morphology under monolayer culture conditions. Flow cytometric analysis demonstrated that bMSC populations were positive for MSC markers CD29 and CD73 and pluripotency markers OCT4 and NANOG; whereas, were negative for hematopoietic markers CD34 and CD45. Levels of mRNA of hepatic genes α-fetoprotein (AFP), albumin (ALB), alpha1 antitrypsin (α1AT), connexin 32 (CNX32), tyrosine aminotransferase (TAT) and cytochrome P450 (CYP3A4) were up-regulated in bMSC during a 28-Day period of hepatogenic differentiation. Functional analyses in differentiated bMSC cultures evidenced an increase (P < 0.05) in albumin and urea production and glycogen storage. bMSC cultured under neurogenic conditions expressed NESTIN and MAP2 proteins at 24 h of culture; whereas, at 144 h also expressed TRKA and PrPC. Levels of MAP2 and TRKA mRNA were up-regulated at the end of the differentiation period. Conversely, bMSC expressed lower levels of NANOG mRNA during both hepatogenic and neurogenic differentiation processes. Conclusion: The expression patterns of linage-specific markers and the production of functional metabolites support the potential for hepatogenic and neurogenic differentiation of bMSC isolated from BM of abattoir-derived fetuses. The simplicity of isolation and the potential to differentiate into a wide variety of cell lineages lays the foundation for bMSC as an interesting alternative for investigation in MSC biology and eventual applications for regenerative therapy in veterinary medicine.en_US
Patrocinadordc.description.sponsorshipNational Commission for Scientific and Technology Research (Fondecyt) from the Ministry of Education, Government of Chile 11100205en_US
Lenguagedc.language.isoen_USen_US
Publisherdc.publisherBIOMEDen_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectBovine fetusesen_US
Títulodc.titleHepatogenic and neurogenic differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetusesen_US
Document typedc.typeArtículo de revistaen_US


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile