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Monocyte chemotactic protein-3: possible involvement in apical periodontitis chemotaxis

Authordc.contributor.authorDezerega Piwonka, Andrea 
Authordc.contributor.authorOsorio A., Constanza es_CL
Authordc.contributor.authorMardones Peñailillo, Jeannette Isabel es_CL
Authordc.contributor.authorMundi, V. es_CL
Authordc.contributor.authorDutzan Muñoz, Nicolás es_CL
Authordc.contributor.authorFranco Martínez, María Eugenia es_CL
Authordc.contributor.authorGamonal Aravena, Jorge Antonio es_CL
Authordc.contributor.authorOyarzún Droguett, Alejandro es_CL
Authordc.contributor.authorOverall, M. C. es_CL
Authordc.contributor.authorHernández Ríos, Emma 
Cita de ítemdc.identifier.citationInternational Endodontic Journal 2010: 43(10) 902–908es_CL
Identifierdc.identifier.otherDOI: 10.1111/j.1365-2591.2010.01764.x
Abstractdc.description.abstractAim  To study the expression of monocyte chemotactic protein-3 (MCP-3, also known as chemokine CCL-7) in tissue from apical lesions (AL) and to associate MCP-3 expression with symptomatic or asymptomatic apical periodontitis. Methodology  To determine the expression of MCP-3 in AL, biopsies obtained during tooth extraction procedures were fixed, subjected to routine processing and diagnosed as apical granuloma (AG) (n = 7) or radicular cyst (RC) (n = 5). As controls, apical periodontal ligament (PDL) specimens from healthy premolars extracted for orthodontics reasons were included (n = 7). All specimens were immunostained for MCP-3 and examined under a light microscope. In addition, homogenates from AL (n = 14) and healthy PDL samples (n = 7) were studied through immunowestern blot. Finally, periapical exudates samples were collected from root canals of teeth having diagnosis of symptomatic (n = 14) and asymptomatic apical periodontitis (n = 14) during routine endodontic treatments and analysed by immunowestern blot and densitometry. Results  MCP-3 was detected in AG and RC and localized mainly to inflammatory leucocytes, whereas no expression was observed in healthy PDLs. MCP-3 was also detected in periapical exudate, and its levels were significantly higher in symptomatic than in asymptomatic apical periodontitis. Conclusions  MCP-3 was expressed in AL and its levels associated with clinical symptoms. MCP-3 might play a role in disease pathogenesis, possibly by stimulating mononuclear chemotaxis.es_CL
Patrocinadordc.description.sponsorshipThis study was supported by DI 07/02-2 and FONDECYT 1090461 funding. The authors express their gratitude to Leslie Henrı´quez for her excellent technical assistance, to Consuelo Fresno for her collaboration in sample collection, and to the Endodontic Society of Chile for its continuous support.es_CL
Publisherdc.publisherJohn Wiley & Sonses_CL
Keywordsdc.subjectApical periodontitises_CL
Títulodc.titleMonocyte chemotactic protein-3: possible involvement in apical periodontitis chemotaxises_CL
Document typedc.typeArtículo de revistaes_CL

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