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Chemokine Monocyte Chemoattractant Protein-3 in Progressive Periodontal Lesions in Patients With Chronic Periodontitis

Authordc.contributor.authorDezerega Piwonka, Andrea 
Authordc.contributor.authorPozo, Patricia es_CL
Authordc.contributor.authorHernández Ríos, Emma es_CL
Authordc.contributor.authorOyarzún Droguett, Alejandro es_CL
Authordc.contributor.authorRivera Escobar, Oriana es_CL
Authordc.contributor.authorDutzan Muñoz, Nicolás es_CL
Authordc.contributor.authorGutiérrez Fernández, Ana es_CL
Authordc.contributor.authorOverall, Christopher M. es_CL
Authordc.contributor.authorGarrido, Mauricio es_CL
Authordc.contributor.authorAlcota Rojas, Marcela es_CL
Authordc.contributor.authorOrtiz, Emilio es_CL
Authordc.contributor.authorGamonal Aravena, Jorge Antonio 
Cita de ítemdc.identifier.citationJournal of Periodontology 2010;81(2)267-276es_CL
Identifierdc.identifier.otherDOI 10.1902/jop.2009.090406
Abstractdc.description.abstractBackground: Chemokines are central in the activation and direction of leukocyte subsets to target tissues. However, the monocyte chemoattractant protein-3 (MCP-3) has not been associated with chronic periodontitis. Chronic periodontitis is an infection showing episodic supporting tissue destruction. The aim of this study is to determine the levels and expression of MCP-3 in periodontal sites characterized by active periodontal connective tissue destruction. Methods: The study population consisted of 15 patients with a progression of periodontitis (15 of 56 patients), 18 patients with chronic periodontitis, and 10 healthy subjects without periodontal disease. As determined by the tolerance method, the 15 patients with moderate to advanced chronic periodontitis showed a progression of periodontitis over a 4-month period. Periodontitis was characterized by at least six sites with a probing depth ≥5 mm, clinical attachment level ≥3 mm, and radiographic bone loss. Gingival crevicular fluid was collected using a paper strip. The total protein concentration was determined. An enzyme-linked immunosorbent assay was performed to determine the total amount of MCP-3, and an immunoWestern blot was conducted to assess molecular MCP-3 forms. To determine the MCP-3 expression by immunohistochemistry, gingival biopsies were obtained from patients with chronic periodontitis and healthy subjects during third-molar extraction surgery. Statistical analyses were performed using statistical software. Data were expressed as subject means ± SD, using the χ2 and Student t tests. Results: The total amount and concentration of chemokine MCP-3 were significantly higher in patients with chronic periodontitis than in healthy subjects (8.25 pg versus 0.53 pg, P = 0.006 and 2.95 pg/μl versus 0.45 pg/μl, P = 0.04, respectively). Active sites showed a significantly higher total amount and concentration of MCP-3 than inactive sites (11.12 versus 2.88 pg, P value = 0.005 and 3.95 versus 1.02, P value = 0.005, respectively). Western blot and immunohistochemical staining confirmed the presence of MCP-3 in periodontal disease, with observable differences between patients with chronic periodontitis and healthy subjects. Conclusions: MCP-3 was highly expressed in patients with chronic periodontitis, particularly in those with progressive periodontal lesions. MCP-3 could be involved in the recruitment of inflammatory cells toward periodontal tissues during the progression of the disease.es_CL
Patrocinadordc.description.sponsorshipThis study was supported by the Scientific and Technologic Investigation Resource, Santiago, Chile (project grants 1050518 and 1090046) and by Recalcine Laboratory, Santiago, Chile. Recombinant MCP-3 was provided by Dr. Christopher M. Overall. The authors report no conflict of interest related to this study.es_CL
Publisherdc.publisherAmerican Academy of Periodontologyes_CL
Títulodc.titleChemokine Monocyte Chemoattractant Protein-3 in Progressive Periodontal Lesions in Patients With Chronic Periodontitises_CL
Document typedc.typeArtículo de revistaes_CL

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