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Ultrastructural and biochemical evidence for the presence of mature steroidogenic acute regulatory protein (StAR) in the cytoplasm of human luteal cells

Authordc.contributor.authorSierralta, Walter 
Authordc.contributor.authorKohen Skop, Paulina es_CL
Authordc.contributor.authorCastro, Olga es_CL
Authordc.contributor.authorMuñoz Gallardo, Alex es_CL
Authordc.contributor.authorStrauss III, Jerom F. es_CL
Authordc.contributor.authorDevoto, Luigi es_CL
Admission datedc.date.accessioned2007-05-18T15:53:42Z
Available datedc.date.available2007-05-18T15:53:42Z
Publication datedc.date.issued2005-10-20
Cita de ítemdc.identifier.citationMOLECULAR AND CELLULAR ENDOCRINOLOGYen
Identifierdc.identifier.issn0303-7207
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/123824
Abstractdc.description.abstractThe distribution of the steroidogenic acute regulatory protein (StAR) inside thecal and granulosa-lutein cells of human corpus luteum 9 (CL) was assessed by immunoelectron microscopy. We found greater levels of StAR immunolabeling in steroidogenic cells from early- and mid-than in late luteal phase CL and lower levels in cells from women treated with a GnRH antagonist in the mid-luteal phase. Immunoelectron microscopy revealed significant levels of StAR antigen in the mitochondria and in the cytoplasm of luteal cells. The 30 kDa mature StAR protein was present in both mitochondria and cytosol (post-mitochondrial) fractions from homogenates of CL at different ages, whereas cytochrome c and mitochondrial HSP70 were detected only in the mitochondrial fraction. Therefore, we hypothesized that either appreciable processing of StAR 37 kDa pre-protein occurs outside the mitochondria, or mature StAR protein is selectively released into the cytoplasm after mitochondrial processing. The presence of mature StAR in the cytoplasm is consonant with the notion that StAR acts on the outer mitochondrial membrane to effect sterol import, and that StAR may interact with other cytoplasmic proteins involved in cholesterol metabolism, including hormone sensitive lipase.en
Lenguagedc.language.isoenen
Publisherdc.publisherELSEVIER IRELAND LTDen
Keywordsdc.subjectCORPUS-LUTEUMen
Títulodc.titleUltrastructural and biochemical evidence for the presence of mature steroidogenic acute regulatory protein (StAR) in the cytoplasm of human luteal cellsen
Document typedc.typeArtículo de revistaen


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