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Authordc.contributor.authorNavarrete Wallace, Paola es_CL
Authordc.contributor.authorToledo, Isabel es_CL
Authordc.contributor.authorMardones, Pamela es_CL
Authordc.contributor.authorOpazo, Rafael es_CL
Authordc.contributor.authorEspejo Torres, Romilio es_CL
Authordc.contributor.authorRomero Ormazábal, Jaime 
Admission datedc.date.accessioned2010-11-23T18:34:32Z
Available datedc.date.available2010-11-23T18:34:32Z
Publication datedc.date.issued2010-09-16
Cita de ítemdc.identifier.citationAQUACULTURE RESEARCH Volume: 41 Issue: 10 Pages: e667-e678 Published: SEP 16 2010en_US
Identifierdc.identifier.issn1355-557X
Identifierdc.identifier.otherDOI: 10.1111/j.1365-2109.2010.02590.x
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/123979
Abstractdc.description.abstractThe application of natural and innocuous compounds has potential in aquaculture as an alternative to antibiotics. We evaluated the effect of diet supplementation with Thymus vulgaris essential oil (TVEO) on the allochthonous microbial composition of rainbow trout. DNA was extracted directly from the intestinal contents, and the V3-V4 regions of the 16S rRNA genes were amplified by PCR. The bacterial composition was analysed using temporal temperature gradient electrophoresis (TTGE). No significant changes (P > 0.05) were detected in the TTGE profiles of TVEO-treated trout compared with the controls. The Dice similarity index revealed a high stability (C-s > 70%) of the intestinal microbiota in both groups during the 5-week period. Sequence analyses of the TTGE bands revealed the same bacterial composition in both groups, with most bacteria belonging to the Proteobacteria and Firmicutes phyla. The in vitro antibacterial activity of TVEO was assessed using a range of normal intestinal isolates and fish pathogens. The inhibitory concentrations for all the tested bacteria were higher than the TVEO levels used in trout, which may explain the in vivo results.en_US
Patrocinadordc.description.sponsorshipThis investigation was supported by a grant (FONDECYT no. 1080480) fromCONICYT-Chile.en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherWILEY-BLACKWELL PUBLISHINGen_US
Keywordsdc.subjectmicrobiotaen_US
Títulodc.titleEffect of Thymus vulgaris essential oil on intestinal bacterial microbiota of rainbow trout, Oncorhynchus mykiss (Walbaum) and bacterial isolatesen_US
Document typedc.typeArtículo de revista


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