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Mouse divalent metal transporter 1 is a copper transporter in HEK293 cells

Authordc.contributor.authorArredondo Olguín, Miguel 
Authordc.contributor.authorMendiburo Seguel, María José es_CL
Authordc.contributor.authorFlores, Sebastián es_CL
Authordc.contributor.authorSingleton, Steven T. es_CL
Authordc.contributor.authorGarrick, Michael D. es_CL
Admission datedc.date.accessioned2014-12-12T13:37:58Z
Available datedc.date.available2014-12-12T13:37:58Z
Publication datedc.date.issued2014
Cita de ítemdc.identifier.citationBiometals (2014) 27:115–123en_US
Identifierdc.identifier.otherDOI 10.1007/s10534-013-9691-6
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/124114
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractDivalent Metal Transporter 1 (DMT1) is an apical Fe transporter in the duodenum and is involved in endosomal Fe export. Four protein isoforms have been described for DMT1, two from mRNA with an iron responsive element (IRE) and two from mRNA without it. The sets of two begin in exon 1A or 2. We have characterized copper transport using mouse 2/-IRE DMT1 during regulated ectopic expression. HEK293 cells carrying a TetR:Hyg element were stably transfected with pDEST31 containing a 2/-IRE construct. 64Cu1? incorporation in doxycycline treated cells exhibited 18.6 and 30.0-fold increases in Cu content, respectively when were exposed to 10 and 100 lM of extracellular Cu. Cu content was*4-fold above that of parent cells or cells carrying just the vector. 64Cu uptake in transfected cells pre-incubated with 5 lM of Cu-His revealed a Vmax and Km of 11.98 ± 0.52 pmol mg protein -1 min-1 and 2.03 ± 0.03 lM, respectively. Doxycycline-stimulated Cu uptake was linear with time. The rates of apical Cu uptake decreased and transepithelial transport increased when intracellular Cu increased. The optimal pH for Cu transport was 6.5; uptake of Cu was temperature dependent. Silver does not inhibit Cu uptake in cells carrying the vector. In conclusion, Cu uptake in HEK293 cells that overexpressed the 2/-IRE isoform of DMT1 transporter supports our earlier contention that DMT1 transports Cu as Cu1+en_US
Patrocinadordc.description.sponsorshipThis work was partially supported by grants FONDECYT 1110080 and # DK59794 from the USA NIH.en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherSpringer Science+Business Mediaen_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectCopperen_US
Títulodc.titleMouse divalent metal transporter 1 is a copper transporter in HEK293 cellsen_US
Document typedc.typeArtículo de revistaen_US


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile