Protective effects of polyunsatutared fatty acids supplementation against testicular damage induced by intermittent hypobaric hypoxia in rats
Author
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Castillo Peñaloza, Rodrigo
Author
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Zepeda, Andrea
Author
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Short, Stefanía
Author
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Figueroa, Elías
Author
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Bustos Obregón, Eduardo
Author
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Farías, Jorge
Admission date
dc.date.accessioned
2015-08-22T19:43:43Z
Available date
dc.date.available
2015-08-22T19:43:43Z
Publication date
dc.date.issued
2015
Cita de ítem
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Journal of Biomedical Science (2015) 22:8
en_US
Identifier
dc.identifier.issn
1021-7770
Identifier
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DOI: 10.1186/s12929-015-0112-8
Identifier
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https://repositorio.uchile.cl/handle/2250/133028
General note
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Artículo de publicación ISI
en_US
Abstract
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Background: Intermittent hypobaric hypoxia (IHH) induces changes in the redox status and structure in rat testis.
These effects may be present in people at high altitudes, such as athletes and miners. Polyunsaturated fatty acids
(PUFA) can be effective in counteracting these oxidative modifications due to their antioxidants properties. The aim
of the work was to test whether PUFA supplementation attenuates oxidative damage in testis by reinforcing the
antioxidant defense system. The animals were divided into four groups (7 rats per group): normobaric normoxia
(~750 tor; pO2 156 mmHg; Nx); Nx + PUFA, supplemented with PUFA (DHA: EPA = 3:1; 0.3 g kg−1 of body weight
per day); hypoxic hypoxia (~428 tor; pO2 90 mmHg; Hx) and, Hx + PUFA. The hypoxic groups were exposed in
4 cycles to 96 h of HH followed by 96 h of normobaric normoxia for 32 days. Total antioxidant capacity (FRAP) and
lipid peroxidation (malondialdehyde, MDA) in plasma and reduced (GSH)/oxidized glutathione (GSSG) ratio, tissue
lipid peroxidation (TBARS) and antioxidant enzymes activity were assessed at the end of the study in testis. Also,
SIRTUIN 1 and HIF-1 protein expression in testis were determined.
Results: IHH increased lipid peroxidation in plasma and HIF-1 protein levels in testis. In addition, IHH reduced FRAP
levels in plasma, antioxidant enzymes activities and SIRTUIN 1 protein levels in testis. PUFA supplementation attenuated
these effects, inducing the increases in FRAP, in the antioxidant enzymes activity and HIF-1 levels.
Conclusions: These results suggest that the IHH model induces a prooxidant status in plasma and testis. The
molecular protective effect of PUFA may involve the induction of an antioxidant mechanism.
en_US
Patrocinador
dc.description.sponsorship
FONDECYT 11110426
, CONICYT doctoral fellowship and
DIUFRO grant DI13-2014