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Authordc.contributor.authorCastillo Peñaloza, Rodrigo 
Authordc.contributor.authorZepeda, Andrea 
Authordc.contributor.authorShort, Stefanía 
Authordc.contributor.authorFigueroa, Elías 
Authordc.contributor.authorBustos Obregón, Eduardo 
Authordc.contributor.authorFarías, Jorge 
Admission datedc.date.accessioned2015-08-22T19:43:43Z
Available datedc.date.available2015-08-22T19:43:43Z
Publication datedc.date.issued2015
Cita de ítemdc.identifier.citationJournal of Biomedical Science (2015) 22:8en_US
Identifierdc.identifier.issn1021-7770
Identifierdc.identifier.otherDOI: 10.1186/s12929-015-0112-8
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/133028
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractBackground: Intermittent hypobaric hypoxia (IHH) induces changes in the redox status and structure in rat testis. These effects may be present in people at high altitudes, such as athletes and miners. Polyunsaturated fatty acids (PUFA) can be effective in counteracting these oxidative modifications due to their antioxidants properties. The aim of the work was to test whether PUFA supplementation attenuates oxidative damage in testis by reinforcing the antioxidant defense system. The animals were divided into four groups (7 rats per group): normobaric normoxia (~750 tor; pO2 156 mmHg; Nx); Nx + PUFA, supplemented with PUFA (DHA: EPA = 3:1; 0.3 g kg−1 of body weight per day); hypoxic hypoxia (~428 tor; pO2 90 mmHg; Hx) and, Hx + PUFA. The hypoxic groups were exposed in 4 cycles to 96 h of HH followed by 96 h of normobaric normoxia for 32 days. Total antioxidant capacity (FRAP) and lipid peroxidation (malondialdehyde, MDA) in plasma and reduced (GSH)/oxidized glutathione (GSSG) ratio, tissue lipid peroxidation (TBARS) and antioxidant enzymes activity were assessed at the end of the study in testis. Also, SIRTUIN 1 and HIF-1 protein expression in testis were determined. Results: IHH increased lipid peroxidation in plasma and HIF-1 protein levels in testis. In addition, IHH reduced FRAP levels in plasma, antioxidant enzymes activities and SIRTUIN 1 protein levels in testis. PUFA supplementation attenuated these effects, inducing the increases in FRAP, in the antioxidant enzymes activity and HIF-1 levels. Conclusions: These results suggest that the IHH model induces a prooxidant status in plasma and testis. The molecular protective effect of PUFA may involve the induction of an antioxidant mechanism.en_US
Patrocinadordc.description.sponsorshipFONDECYT 11110426 , CONICYT doctoral fellowship and DIUFRO grant DI13-2014en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherBioMed Centralen_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectIntermittent hypoxiaen_US
Keywordsdc.subjectTestisen_US
Keywordsdc.subjectOxidative stressen_US
Keywordsdc.subjectPUFAen_US
Keywordsdc.subjectSIRTUIN 1en_US
Títulodc.titleProtective effects of polyunsatutared fatty acids supplementation against testicular damage induced by intermittent hypobaric hypoxia in ratsen_US
Document typedc.typeArtículo de revista


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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile