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Authordc.contributor.authorPérez, Claudio A. 
Authordc.contributor.authorRabanales, Ramón 
Authordc.contributor.authorRojas Alcayaga, Gonzalo 
Authordc.contributor.authorLarrondo Lillo, Milton 
Authordc.contributor.authorEscobar Álvarez, Alejandro 
Authordc.contributor.authorLópez, Mercedes N. 
Authordc.contributor.authorSalazar Onfray, Flavio 
Authordc.contributor.authorAlfaro Lucero, Jorge 
Authordc.contributor.authorGonzález, Fermin E. 
Admission datedc.date.accessioned2016-06-16T22:43:37Z
Available datedc.date.available2016-06-16T22:43:37Z
Publication datedc.date.issued2016
Cita de ítemdc.identifier.citationJ Oral Pathol Med (2016) 45: 127–135en_US
Identifierdc.identifier.otherDOI: 10.1111/jop.12338
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/138929
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractObjectiveGraft-versus-host disease (GVHD) is one of the main complications after haematopoietic stem cell transplantation. Clinical features of GVHD include either an acute (aGVHD) or a chronic (cGVHD) condition that affects locations such as the oral mucosa. While the involvement of the host's dendritic cells (DCs) has been demonstrated in aGVHD, the origin (donor/host) and mechanisms underlying oral cGVHD have not been completely elucidated. In this study, we intend to determine the origin of DCs present in mucosal tissue biopsies from the oral cavity of transplanted patients affected by cGVHD. MethodsWe purified DCs, from oral biopsies of three patients with cGVHD, through immunobeads and subsequently performed DNA extraction. The origin of the obtained DCs was determined by PCR amplification of 13 informative short tandem repeat (STR) alleles. We also characterised the DCs phenotype and the inflammatory infiltrate from biopsies of two patients by immunohistochemistry. ResultsClinical and histological features of the biopsies were concordant with oral cGVHD. We identified CD11c-, CD207- and CD1a-positive cells in the epithelium and beneath the basal layer. Purification of DCs from the mucosa of patients affected by post-transplantation cGVHD was >95%. PCR-STR data analysis of DCs DNA showed that 100% of analysed cells were of donor origin in all of the evaluated patients. ConclusionOur results demonstrate that resident DCs isolated from the oral tissue of allotransplanted patients affected by cGVHD are originated from the donor. Further research will clarify the role of DCs in the development and/or severity of oral cGVHD.en_US
Patrocinadordc.description.sponsorshipUniversity of Chile Clinical Hospital, Millennium Science Initiative from the Ministry for the Economy, Development and Tourismen_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherJohn Wiley & Sonsen_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectimmunotherapyen_US
Keywordsdc.subjectcriteriaen_US
Keywordsdc.subjectchronic gvhden_US
Keywordsdc.subjectt-cellsen_US
Keywordsdc.subjectclinical-trialsen_US
Keywordsdc.subjectepidermal langerhans cellsen_US
Keywordsdc.subjectbone-marrow transplantationen_US
Keywordsdc.subjectworking group-reporten_US
Keywordsdc.subjectconsensus development projecten_US
Keywordsdc.subjectmixed hematopoietic chimerismen_US
Títulodc.titleDendritic cell chimerism in oral mucosa of transplanted patients affected by graft-versus-host diseaseen_US
Document typedc.typeArtículo de revistaen_US


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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile