PCR Restriction Fragment Length Polymorphism Analyses of V. Parahaemolyticus MAM-7 Virulence Gene in Clinical and Environmental Strains
Author
dc.contributor.author
Elola-López, Ana
Author
dc.contributor.author
Esquivel, María José
Author
dc.contributor.author
Muñoz Bergmann, Cristian
Author
dc.contributor.author
Beltrán, Sebastián
Author
dc.contributor.author
Osorio Abarzúa, Carlos Gonzalo
Author
dc.contributor.author
Trombert, Annette N.
Admission date
dc.date.accessioned
2017-09-28T20:20:29Z
Available date
dc.date.available
2017-09-28T20:20:29Z
Publication date
dc.date.issued
2015
Cita de ítem
dc.identifier.citation
Electronic Journal of Biology, 2015, Vol.11(3): 119-125
es_ES
Identifier
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1860-3122
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/145118
Abstract
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Virulent and non-virulent Vibrio parahaemolyticus
(V. parahaemolyticus) strains coexist together
in seawater. A PCR–restriction fragment length
polymorphism (PCR-RFLP) technique could
differentiate between clinical (virulent) and
environmental V. parahaemolyticus strains. MAM-
7 corresponds to a virulence gene described in V.
parahaemolyticus and that participates in initial
stages of pathogen gut colonization. The objective
of our study is to evaluate if PCR-RFLP analyses of
MAM-7 gene can discriminate between clinical and
environmental V. parahaemolyticus strains.
Ten V. parahaemolyticus clinical isolates and nine V.
parahaemolyticus environmental isolates were used
to obtain genomic DNA. A 2619 bp PCR product
from MAM-7 gene was digested with HindIII and
AcuI restriction enzymes revealing a characteristic
common pattern in 100% of V. parahaemolyticus
clinical isolates. These patterns were absolutely
different of those obtained from environmental
isolates. PCR of toxin related genes (tdh and trh)
showed that only clinical isolates were tdh+.
As a conclusion, PCR-RFLP of V. parahaemolyticus
MAM-7 gene could discriminate between clinical
tdh+ isolates and environmental ones and could
complement other diagnostic tools to detect and
classify virulent strains. However, it is still necessary
to analyze more samples of V. parahaemolyticus.
Thus, while these results are promising, this study
corresponds to preliminary work.