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Hyperosmotic stress stimulates autophagy via polycystin-2

Authordc.contributor.authorPeña Oyarzún, Daniel 
Authordc.contributor.authorTroncoso Cotal, Rodrigo 
Authordc.contributor.authorKretschmar, Catalina 
Authordc.contributor.authorHernando, Cecilia 
Authordc.contributor.authorBudini, Mauricio 
Authordc.contributor.authorMorselli, Eugenia 
Authordc.contributor.authorLavandero González, Sergio 
Authordc.contributor.authorCriollo Céspedes, Alfredo 
Cita de ítemdc.identifier.citationOncotarget, 2017, Vol. 8, (No. 34): 55984-55997es_ES
Abstractdc.description.abstractVarious intracellular mechanisms are activated in response to stress, leading to adaptation or death. Autophagy, an intracellular process that promotes lysosomal degradation of proteins, is an adaptive response to several types of stress. Osmotic stress occurs under both physiological and pathological conditions, provoking mechanical stress and activating various osmoadaptive mechanisms. Polycystin-2 (PC2), a membrane protein of the polycystin family, is a mechanical sensor capable of activating the cell signaling pathways required for cell adaptation and survival. Here we show that hyperosmotic stress provoked by treatment with hyperosmolar concentrations of sorbitol or mannitol induces autophagy in HeLa and HCT116 cell lines. In addition, we show that mTOR and AMPK, two stress sensor proteins involved modulating autophagy, are downregulated and upregulated, respectively, when cells are subjected to hyperosmotic stress. Finally, our findings show that PC2 is required to promote hyperosmotic stress-induced autophagy. Downregulation of PC2 prevents inhibition of hyperosmotic stress-induced mTOR pathway activation. In conclusion, our data provide new insight into the role of PC2 as a mechanosensor that modulates autophagy under hyperosmotic stress conditions.es_ES
Patrocinadordc.description.sponsorshipFondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT) 11130285 1161123 1160820 1140908 Fondo de Financiamiento de Centros de Investigacion en Areas Prioritarias (FONDAP) 15130011 Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) 00002991 International Centre for Genetic Engineering and Biotechnology (ICGEB) CRP/CH113-04RG CRP/CHL16-06es_ES
Publisherdc.publisherImpact Journalses_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Keywordsdc.subjectHyperosmotic stresses_ES
Títulodc.titleHyperosmotic stress stimulates autophagy via polycystin-2es_ES
Document typedc.typeArtículo de revistaes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile