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Endogenous overexpression of an active phosphorylated form of DNA polymerase beta under oxidative stress in Trypanosoma cruzi

Authordc.contributor.authorRojas, Diego A. 
Authordc.contributor.authorUrbina, Fabiola 
Authordc.contributor.authorMoreira Ramos, Sandra 
Authordc.contributor.authorCastillo, Christian 
Authordc.contributor.authorKemmerling Weis, Ulrike 
Authordc.contributor.authorLapier, Michel 
Authordc.contributor.authorMaya Arango, Juan 
Authordc.contributor.authorSolari Illescas, Aldo 
Authordc.contributor.authorMaldonado Maldonado, Edio 
Admission datedc.date.accessioned2018-07-19T22:18:19Z
Available datedc.date.available2018-07-19T22:18:19Z
Publication datedc.date.issued2018
Cita de ítemdc.identifier.citationPlos Negl Trop Dis 12(2): e0006220es_ES
Identifierdc.identifier.other10.1371/journal.pntd.0006220
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/150028
Abstractdc.description.abstractTrypanosoma cruzi is exposed during its life to exogenous and endogenous oxidative stress, leading to damage of several macromolecules such as DNA. There are many DNA repair pathways in the nucleus and mitochondria (kinetoplast), where specific protein complexes detect and eliminate damage to DNA. One group of these proteins is the DNA polymerases. In particular, Tc DNA polymerase beta participates in kinetoplast DNA replication and repair. However, the mechanisms which control its expression under oxidative stress are still unknown. Here we describe the effect of oxidative stress on the expression and function of Tc DNA polymerase beta To this end parasite cells (epimastigotes and trypomastigotes) were exposed to peroxide during short periods of time. Tc DNA polymerase beta which was associated physically with kinetoplast DNA, showed increased protein levels in response to peroxide damage in both parasite forms analyzed. Two forms of DNA polymerase beta were identified and overexpressed after peroxide treatment. One of them was phosphorylated and active in DNA synthesis after renaturation on polyacrylamide electrophoresis gel. This phosphorylated form showed 3-4-fold increase in both parasite forms. Our findings indicate that these increments in protein levels are not under transcriptional control because the level of Tc DNA polymerase beta mRNA is maintained or slightly decreased during the exposure to oxidative stress. We propose a mechanism where a DNA repair pathway activates a cascade leading to the increment of expression and phosphorylation of Tc DNA polymerase 13 in response to oxidative damage, which is discussed in the context of what is known in other trypanosomes which lack transcriptional control.es_ES
Patrocinadordc.description.sponsorshipFondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT) 1120507es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherPublic Library Sciencees_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourcePlos Neglected Tropical Diseaseses_ES
Títulodc.titleEndogenous overexpression of an active phosphorylated form of DNA polymerase beta under oxidative stress in Trypanosoma cruzies_ES
Document typedc.typeArtículo de revistaes_ES
Catalogueruchile.catalogadortjnes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile