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Comparative studies on glucose phosphorylating isoenzymes of vertebrates. Identification and characterization of amphibian liver hexokinases

Authordc.contributor.authorUreta, Tito 
Authordc.contributor.authorRadojković, Jasna 
Authordc.contributor.authorDíaz, Nelson 
Authordc.contributor.authorSlebe, Juan C. 
Authordc.contributor.authorLozano, Carlos 
Admission datedc.date.accessioned2018-12-20T14:08:11Z
Available datedc.date.available2018-12-20T14:08:11Z
Publication datedc.date.issued1978
Cita de ítemdc.identifier.citationArchives of Biochemistry and Biophysics, Volumen 186, Issue 2, 2018, Pages 235-247
Identifierdc.identifier.issn10960384
Identifierdc.identifier.issn00039861
Identifierdc.identifier.other10.1016/0003-9861(78)90432-0
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/154127
Abstractdc.description.abstractGlucose phosphorylating activities were measured in liver extracts from two urodeles and twenty-six anurans. Fractionation on diethylaminoethyl-cellulose columns of liver extracts from these amphibians permitted the recognition of four hexokinases which are called A, B, C, and D. However, any given amphibian displays only three liver hexokinases and the profiles so far observed are either of the type A-B-D or C-B-D. The distribution of the amphibians in either type of pattern does not show any simple taxonomic relationship. A wide generic and specific, but not individual, variation of the relative proportion of each isoenzyme was observed. Hexokinases A and B were shown to be low Km glucose isoenzymes (0.06 and 0.15 mm glucose, respectively) with normal hyperbolic kinetics. Hexokinase C, also a low Km isoenzyme (0.05 mm) was found to be inhibited by excess substrate at physiological levels of glucose. Hexokinases A, B, and C were able to phosphorylate fructose, mannose, and 2-deoxygluc
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceArchives of Biochemistry and Biophysics
Keywordsdc.subjectBiophysics
Keywordsdc.subjectBiochemistry
Keywordsdc.subjectMolecular Biology
Títulodc.titleComparative studies on glucose phosphorylating isoenzymes of vertebrates. Identification and characterization of amphibian liver hexokinases
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile