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Evidence of essential arginyl residues in rabbit muscle pyruvate kinase

Authordc.contributor.authorCardemil, Emilio 
Authordc.contributor.authorEyzaguirre, Jaime 
Admission datedc.date.accessioned2018-12-20T14:08:12Z
Available datedc.date.available2018-12-20T14:08:12Z
Publication datedc.date.issued1979
Cita de ítemdc.identifier.citationArchives of Biochemistry and Biophysics, Volumen 192, Issue 2, 2018, Pages 533-538
Identifierdc.identifier.issn10960384
Identifierdc.identifier.issn00039861
Identifierdc.identifier.other10.1016/0003-9861(79)90123-1
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/154132
Abstractdc.description.abstractRabbit muscle pyruvate kinase is inactivated by 2,3-butanedione in borate buffer. The inactivation follows pseudo-first-order kinetics with a calculated second-order rate constant of 4.6 m-1 min-1. The modification can be reversed with almost total recovery of activity by elimination of the butanedione and borate buffer, suggesting that only arginyl groups are modified; this result agrees with the loss of arginine detected by amino acid analysis of the modified enzyme. Using the kinetic data, it was estimated that the reaction of a single butanedione molecule per subunit of the enzyme is enough to completely inactivate the protein. The inactivation is partially prevented by phosphoenolpyruvate in the presence of K+ and Mg2+, but not by the competitive inhibitors lactate and bicarbonate. These findings point to an essential arginyl residue being located near the phosphate binding site of phosphoenolpyruvate. © 1979.
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceArchives of Biochemistry and Biophysics
Keywordsdc.subjectBiophysics
Keywordsdc.subjectBiochemistry
Keywordsdc.subjectMolecular Biology
Títulodc.titleEvidence of essential arginyl residues in rabbit muscle pyruvate kinase
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile