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Authordc.contributor.authorGallardo, C. E. 
Authordc.contributor.authorCorrea, C. 
Authordc.contributor.authorMorales, P. 
Authordc.contributor.authorSáez, P. A. 
Authordc.contributor.authorPastenes, L. 
Authordc.contributor.authorMéndez, M. A. 
Admission datedc.date.accessioned2018-12-20T14:53:17Z
Available datedc.date.available2018-12-20T14:53:17Z
Publication datedc.date.issued2012
Cita de ítemdc.identifier.citationMolecular Ecology Resources (2012) 12, 1090–1096
Identifierdc.identifier.issn1755098X
Identifierdc.identifier.issn17550998
Identifierdc.identifier.other10.1111/1755-0998.12013
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/157313
Abstractdc.description.abstractThe use of nondestructive methods for obtaining DNA from amphibians (e.g. buccal swabs) allows genetic studies to be performed without affecting the survival of the studied individuals. In this study, we compared two methods of nondestructive DNA sampling, buccal swabs and interdigital membrane or toe-clipping, in several amphibian species of different size: Rhinella spinulosa, R.similar to atacamensis, six species of the genus Telmatobius and Pleurodema thaul. We evaluated the integrity of the DNA extracted by sequencing fragments of mitochondrial and nuclear genes and by generating amplified fragment length polymorphisms markers (AFLPs). In all cases, we obtained an adequate amount of DNA (mean range 55 similar to 298 similar to ng/mu L). We obtained identical DNA sequences from buccal swab and interdigital membrane/toe-clip for all individuals. The differences in the coding of AFLP markers between the tissues were similar to those reported for replicas of the same type of sample in similar analyses in other species of amphibians. In conclusion, the use of buccal swabs is a trustworthy and inexpensive method to obtain DNA for mitochondrial and nuclear sequencing and AFLP analyses. Given the types of markers evaluated, buccal swabs may be used for phylogenetic, phylogeographic and population genetic studies, even in small amphibians (<33 similar to mm).
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceMolecular Ecology Resources
Keywordsdc.subject12S
Keywordsdc.subject16S
Keywordsdc.subjectAFLPs
Keywordsdc.subjectAmphibians
Keywordsdc.subjectControl region
Keywordsdc.subjectNoninvasive sampling
Keywordsdc.subjectRag1
Keywordsdc.subjectRhodopsin
Títulodc.titleValidation of a cheap and simple nondestructive method for obtaining AFLPs and DNA sequences (mitochondrial and nuclear) in amphibians
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorlaj
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile