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Authordc.contributor.authorEncinas, M. Victoria 
Authordc.contributor.authorLissi Gervaso, Eduardo A. 
Authordc.contributor.authorVásquez, M. 
Authordc.contributor.authorOlea, Andrés F. 
Authordc.contributor.authorSILVA, E. 
Admission datedc.date.accessioned2018-12-20T15:05:11Z
Available datedc.date.available2018-12-20T15:05:11Z
Publication datedc.date.issued1989
Cita de ítemdc.identifier.citationPhotochemistry and Photobiology, Volumen 49, Issue 5, 2018, Pages 557-563
Identifierdc.identifier.issn17511097
Identifierdc.identifier.issn00318655
Identifierdc.identifier.other10.1111/j.1751-1097.1989.tb08424.x
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/157712
Abstractdc.description.abstractAbstract The quenching of the benzophenone triplet by lysozyme and its constituent amino acids in aqueous solutions have been studied. Native lysozyme quenches the benzophenone triplet with a high rate constant, 4 × 109M‐1s‐1. The quenching process takes place with production of significant amounts of free ketyl radicals, φketyl=0.56, but with a very low benzophenone consumption yield (0.022). The consumption yield is considerably smaller than that observed for the free amino acids. This difference can be explained in terms of a dominant back hydrogen transfer to the protein in the disproportionation of the free radicals produced. Reduced and carboxymethylated lysozyme shows a higher quenching rate (7.8 × 109M‐1s‐1) and a larger benzophenone consumption yield (0.07). The deactivation of the benzophenone triplet by the native protein leads to its inactivation, with a quantum yield of 0.01. Tryptophan and arginine residues are destroyed with a quantum yield of 0.01. In the modified enzym
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourcePhotochemistry and Photobiology
Keywordsdc.subjectBiochemistry
Keywordsdc.subjectPhysical and Theoretical Chemistry
Títulodc.titlePHOTOINTERACTION OF BENZOPHENONE TRIPLET WITH LYSOZYME
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile