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A comparative study of fish species identification by gel isoelectrofocusing, two-dimensional gel electrophoresis, and capillary zone electrophoresis

Authordc.contributor.authorValenzuela, María Antonieta 
Authordc.contributor.authorGamarra, Norma 
Authordc.contributor.authorGómez, Luis 
Authordc.contributor.authorKettlun, Ana María 
Authordc.contributor.authorSardón, Marcia 
Authordc.contributor.authorPérez, Luz María 
Authordc.contributor.authorVinagre, Julia 
Authordc.contributor.authorGuzman, Norberto A. 
Admission datedc.date.accessioned2018-12-20T15:09:22Z
Available datedc.date.available2018-12-20T15:09:22Z
Publication datedc.date.issued1999
Cita de ítemdc.identifier.citationJournal of Capillary Electrophoresis and Microchip Technology, Volumen 6, Issue 3-4, 2018, Pages 85-91
Identifierdc.identifier.issn10795383
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/158041
Abstractdc.description.abstractA comparative study of fish species identification was accomplished using three different electrophoretic techniques. Sarcoplasmic proteins were extracted from three related fish species and subjected to gel isoelectrofocusing (IEF), two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and capillary zone electrophoresis (CZE). The fish species-Genypterus chilensis, Genypterus blacodes, and Genypterus maculatus-were from the Ophidiidae family. The three electrophoretic techniques provided suitable fish species identification. Nevertheless, CZE demonstrated several advantages over the other two conventional techniques. Some of the benefits include the use of small amounts of reagents; short separation times, permitting fast comparative analysis; data reproducibility; and ease with which the technique is performed.
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceJournal of Capillary Electrophoresis and Microchip Technology
Keywordsdc.subjectAnimal proteins
Keywordsdc.subjectCapillary electrophoresis
Keywordsdc.subjectElectrophoretic techniques
Keywordsdc.subjectFingerprinting
Keywordsdc.subjectFish species identification
Keywordsdc.subjectIsoelectric focusing
Keywordsdc.subjectTwo-dimensional electrophoresis
Títulodc.titleA comparative study of fish species identification by gel isoelectrofocusing, two-dimensional gel electrophoresis, and capillary zone electrophoresis
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile