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Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora

Authordc.contributor.authorTello, Mario 
Authordc.contributor.authorSeelenfreund, Daniela 
Authordc.contributor.authorLobos, Sergio 
Authordc.contributor.authorGaskell, Jill 
Authordc.contributor.authorCullen, Daniel 
Authordc.contributor.authorVicuña, Rafael 
Admission datedc.date.accessioned2018-12-20T15:20:40Z
Available datedc.date.available2018-12-20T15:20:40Z
Publication datedc.date.issued2001
Cita de ítemdc.identifier.citationFEMS Microbiology Letters, Volumen 199, Issue 1, 2018, Pages 91-96
Identifierdc.identifier.issn03781097
Identifierdc.identifier.other10.1016/S0378-1097(01)00156-2
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/158857
Abstractdc.description.abstractGenetic analyses of the lignin-degrading fungus Ceriporiopsis subvermispora is complicated by a dikaryotic nuclear condition and the absence of spore forms. Previous investigations had identified a family of closely related sequences encoding manganese peroxidase (MnP), but the relationship between genes and allelic variants could not be experimentally established. Addressing this issue, homokaryotic derivatives of C. subvermipora strain FP105752 were isolated from regenerated protoplasts. Designated CsA and CsB, their homokaryotic nature was established by polymerase chain reaction amplification and sequence analysis of the allelic variants of three MnP genes. Isoelectrofocusing revealed fewer MnP isoenzymes in filtrates of homokaryon cultures relative to the parental strain. The homokaryotic strains will simplify genetic analyses, particularly the identification of new genes. © 2001 Federation of European Microbiological Societies.
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceFEMS Microbiology Letters
Keywordsdc.subjectBasidiomycete
Keywordsdc.subjectCeriporiopsis subvermispora
Keywordsdc.subjectHomokaryon
Keywordsdc.subjectLigninolytic
Keywordsdc.subjectManganese peroxidase
Títulodc.titleIsolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile