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Authordc.contributor.authorParra Flores, Julio 
Authordc.contributor.authorCerda Leal, Fabiola 
Authordc.contributor.authorContreras, Alejandra 
Authordc.contributor.authorValenzuela Riffo, Nicole 
Authordc.contributor.authorRodríguez, Alejandra 
Authordc.contributor.authorAguirre, Juan 
Admission datedc.date.accessioned2019-01-10T19:43:23Z
Available datedc.date.available2019-01-10T19:43:23Z
Publication datedc.date.issued2018
Cita de ítemdc.identifier.citationFrontiers in Microbiology July 2018 | Volume 9 | Article 1708es_ES
Identifierdc.identifier.other10.3389/fmicb.2018.01708
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/159341
Abstractdc.description.abstractThe objective of this study was to evaluate the presence of Cronobacter sakazakii and microbiological parameters in dairy products associated with a food alert. Ninety dairy product samples were analyzed, including seven commercial brands and two product types (liquid and powdered) from four countries. Aerobic plate count (APC) and Enterobacteriaceae count were performed according to Chilean standards. Cronobacter spp. and C. sakazakii were identified by polymerase chain reaction real time amplification of rpoB and cgcA genes and the genotype by multilocus sequence typing. Eighty-eight percent of dairy products showed APC higher than the detection limit. Fifty percent of liquid commercial brand samples contained APC: 2.6, 2.3, 1.1, and 2.9 CFU/mL in brands A, C, E, and G, respectively. Results for powdered commercial brands were 3.0, 3.6, and 5.7 CFU/g in brands B, D, and F, respectively. Maximum count (5.7 CFU/g) occurred in brand F dairy product manufactured in Chile. Enterobacteriaceae were found in 55% of the samples, 64% in liquid and 51% in powdered commercial brands. In 50% of brands B, D, and E, samples contained 2.9, 2.8, and 2.7 log CFU/g, respectively. Only liquid commercial brands from the United States had Enterobacteriaceae values between 0.1 and 4.5 CFU/mL. Seventeen suspicious strains were isolated and nine were identified as Enterobacter spp. Only eight suspicious strains from four powdered commercial brands (Chile and Singapore) were confirmed as C. sakazakii by rpoB and cgcA gene amplification and fusA sequencing. C. sakazakii prevalence in the analyzed samples was 8.8%. There were 11% of powdered milk brands that contained APC between 4.0 and 4.7 log CFU/g and 55% of the samples contained Enterobacteriaceae. C. sakazakii was found in dairy products manufactured in Chile and Singapore. On the basis of this information, the Chilean Ministry of Health (RSA) decreed a national and international food alert and recalled all the product batches that resulted positive in the present study from supermarkets and pharmacies.es_ES
Patrocinadordc.description.sponsorshipResearch Directorate of the Universidad del Bio-Bio 161720 3/R 091824/R GI 171220/EFes_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherFrontiers Mediaes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourceFrontiers in Microbiologyes_ES
Keywordsdc.subjectCronobacter sakazakiies_ES
Keywordsdc.subjectFood alertes_ES
Keywordsdc.subjectMicrobiological parameterses_ES
Keywordsdc.subjectPowdered infant formulaes_ES
Keywordsdc.subjectLiquid dairy formulaes_ES
Títulodc.titleCronobacter sakazakii and microbiological parameters in dairy formulas associated with a food alert in Chilees_ES
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorlajes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile