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Cronobacter sakazakii and microbiological parameters in dairy formulas associated with a food alert in Chile

Autordc.contributor.authorParra Flores, Julio 
Autordc.contributor.authorCerda Leal, Fabiola 
Autordc.contributor.authorContreras, Alejandra 
Autordc.contributor.authorValenzuela Riffo, Nicole 
Autordc.contributor.authorRodríguez, Alejandra 
Autordc.contributor.authorAguirre, Juan 
Fecha ingresodc.date.accessioned2019-01-10T19:43:23Z
Fecha disponibledc.date.available2019-01-10T19:43:23Z
Fecha de publicacióndc.date.issued2018
Cita de ítemdc.identifier.citationFrontiers in Microbiology July 2018 | Volume 9 | Article 1708es_ES
Identificadordc.identifier.other10.3389/fmicb.2018.01708
Identificadordc.identifier.urihttp://repositorio.uchile.cl/handle/2250/159341
Resumendc.description.abstractThe objective of this study was to evaluate the presence of Cronobacter sakazakii and microbiological parameters in dairy products associated with a food alert. Ninety dairy product samples were analyzed, including seven commercial brands and two product types (liquid and powdered) from four countries. Aerobic plate count (APC) and Enterobacteriaceae count were performed according to Chilean standards. Cronobacter spp. and C. sakazakii were identified by polymerase chain reaction real time amplification of rpoB and cgcA genes and the genotype by multilocus sequence typing. Eighty-eight percent of dairy products showed APC higher than the detection limit. Fifty percent of liquid commercial brand samples contained APC: 2.6, 2.3, 1.1, and 2.9 CFU/mL in brands A, C, E, and G, respectively. Results for powdered commercial brands were 3.0, 3.6, and 5.7 CFU/g in brands B, D, and F, respectively. Maximum count (5.7 CFU/g) occurred in brand F dairy product manufactured in Chile. Enterobacteriaceae were found in 55% of the samples, 64% in liquid and 51% in powdered commercial brands. In 50% of brands B, D, and E, samples contained 2.9, 2.8, and 2.7 log CFU/g, respectively. Only liquid commercial brands from the United States had Enterobacteriaceae values between 0.1 and 4.5 CFU/mL. Seventeen suspicious strains were isolated and nine were identified as Enterobacter spp. Only eight suspicious strains from four powdered commercial brands (Chile and Singapore) were confirmed as C. sakazakii by rpoB and cgcA gene amplification and fusA sequencing. C. sakazakii prevalence in the analyzed samples was 8.8%. There were 11% of powdered milk brands that contained APC between 4.0 and 4.7 log CFU/g and 55% of the samples contained Enterobacteriaceae. C. sakazakii was found in dairy products manufactured in Chile and Singapore. On the basis of this information, the Chilean Ministry of Health (RSA) decreed a national and international food alert and recalled all the product batches that resulted positive in the present study from supermarkets and pharmacies.es_ES
Patrocinadordc.description.sponsorshipResearch Directorate of the Universidad del Bio-Bio 161720 3/R 091824/R GI 171220/EFes_ES
Idiomadc.language.isoenes_ES
Publicadordc.publisherFrontiers Mediaes_ES
Tipo de licenciadc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link a Licenciadc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Fuentedc.sourceFrontiers in Microbiologyes_ES
Palabras clavesdc.subjectCronobacter sakazakiies_ES
Palabras clavesdc.subjectFood alertes_ES
Palabras clavesdc.subjectMicrobiological parameterses_ES
Palabras clavesdc.subjectPowdered infant formulaes_ES
Palabras clavesdc.subjectLiquid dairy formulaes_ES
Títulodc.titleCronobacter sakazakii and microbiological parameters in dairy formulas associated with a food alert in Chilees_ES
Tipo de documentodc.typeArtículo de revistaes_ES
Catalogadoruchile.catalogadorlajes_ES
Indizaciónuchile.indexArtículo de publicación ISIes_ES


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Excepto si se señala otra cosa, la licencia del ítem se describe como Attribution-NonCommercial-NoDerivs 3.0 Chile