Identification of a type II cystatin in Fragaria chiloensis: A proteinase inhibitor differentially regulated during achene development and in response to biotic stress-related stimuli
Author
dc.contributor.author
Aceituno Valenzuela, Uri
Author
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Covarrubias, María Paz
Author
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Aguayo, María Francisca
Author
dc.contributor.author
Valenzuela Riffo, Felipe
Author
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Espinoza, Analía
Author
dc.contributor.author
Gaete Eastman, Carlos
Author
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Godoy Herrera, Raúl
Author
dc.contributor.author
Handford, Michael
Author
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Norambuena Morales, Lorena
Admission date
dc.date.accessioned
2019-01-13T03:40:42Z
Available date
dc.date.available
2019-01-13T03:40:42Z
Publication date
dc.date.issued
2018
Cita de ítem
dc.identifier.citation
Plant Physiology and Biochemistry 129 (2018) 158–167
es_ES
Identifier
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10.1016/j.plaphy.2018.05.021
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/159366
Abstract
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The equilibrium between protein synthesis and degradation is key to maintaining efficiency in different physiological processes. The proteinase inhibitor cystatin regulates protease activities in different developmental and physiological contexts. Here we describe for the first time the identification and the biological function of the cysteine protease inhibitor cystatin of Fragaria chiloensis, FchCYS1. Based on primary sequence and 3D-structural homology modelling, FchCYS1 is a type II phytocystatin with high identity to other cystatins of the Fragaria genus. Both the papain-like and the legumain-like protease inhibitory domains are indeed functional, based on in vitro assays performed with Escherichia coli protein extracts containing recombinant FchCYS1. FchCYS1 is differentially-expressed in achenes of F. chiloensis fruits, with highest expression as the fruit reaches the ripened stage, suggesting a role in preventing degradation of storage proteins that will nourish the embryo during seed germination. Furthermore, FchCYS1 responds transcriptionally to the application of salicylic acid and to mechanical injury, strongly suggesting that FchCYS1 could be involved in the response against pathogen attack. Overall these results point to a role for FchCYS1 in diverse physiological processes in F. chiloensis.
es_ES
Patrocinador
dc.description.sponsorship
PBCT Anillo ACT-1110
FONDECYT
1120289
1170950
1140257
1181198
PAIFAC, Support Program for Research of the Faculty of Sciences, Universidad de Chile
Identification of a type II cystatin in Fragaria chiloensis: A proteinase inhibitor differentially regulated during achene development and in response to biotic stress-related stimuli