Trypanosoma cruzi induces tissue disorganization and destruction of chorionic villi in an ex vivo infection model of human placenta
Author
dc.contributor.author
Duaso, J.
Author
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Rojo, G.
Author
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Cabrera, G.
Author
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Galanti Garrone, Norbel
Author
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Bosco, C.
Author
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Maya Arango, Juan
Author
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Morello Casté, Antonio
Author
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Kemmerling Weis, Ulrike
Admission date
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2019-03-11T13:00:12Z
Available date
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2019-03-11T13:00:12Z
Publication date
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2010
Cita de ítem
dc.identifier.citation
Placenta 31 (2010) 705e711
Identifier
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01434004
Identifier
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10.1016/j.placenta.2010.05.007
Identifier
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https://repositorio.uchile.cl/handle/2250/165057
Abstract
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Congenital Chagas’ disease, endemic in Latin America and also present with lower frequency in other
countries, is associated with premature labor, miscarriage, and placentitis. The mechanism of tissue
invasion and infection of human placenta by the parasite Trypanosoma cruzi (T. cruzi) remains unclear. In
order to explore some morphological aspects of this infection in the placenta, we incubated chorionic
villous explants from normal human placentae ex vivo with the parasite and studied the resulting effects
by immunohistochemical and histochemical methods. Infection of the chorionic villi with the parasite
was confirmed by immunofluoresence and PCR. T. cruzi induces syncytiotrophoblast destruction and
detachment, selective disorganization of basal lamina and disorganization of collagen I in the connective
tissue of villous stroma. These effects are a function of the number of parasites used for the infection. Our
results suggest a participation of the proteolytic activity of the parasite on the placental basal lamina and
connective tissue in the mechanism of infection of the fetus by T. cruzi.