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Author | dc.contributor.author | Mosquera, Jocelyn | |
Author | dc.contributor.author | Armisen Yáñez, Ricardo | |
Author | dc.contributor.author | Zhao, Hongling | |
Author | dc.contributor.author | Rojas, Diego A. | |
Author | dc.contributor.author | Maldonado, Edio | |
Author | dc.contributor.author | Tapia, Julio C. | |
Author | dc.contributor.author | Colombo, Alicia | |
Author | dc.contributor.author | Hayman, Michael J. | |
Author | dc.contributor.author | Marcelain Cubillos, Katherine | |
Admission date | dc.date.accessioned | 2019-03-11T13:01:43Z | |
Available date | dc.date.available | 2019-03-11T13:01:43Z | |
Publication date | dc.date.issued | 2011 | |
Cita de ítem | dc.identifier.citation | Biochemical and Biophysical Research Communications, Volumen 409, Issue 3, 2018, Pages 539-543 | |
Identifier | dc.identifier.issn | 0006291X | |
Identifier | dc.identifier.issn | 10902104 | |
Identifier | dc.identifier.other | 10.1016/j.bbrc.2011.05.040 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/165256 | |
Abstract | dc.description.abstract | Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process. © 2011 Elsevier Inc. | |
Lenguage | dc.language.iso | en | |
Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
Source | dc.source | Biochemical and Biophysical Research Communications | |
Keywords | dc.subject | Aurora A | |
Keywords | dc.subject | Ski | |
Keywords | dc.subject | Ski phosphorylation | |
Título | dc.title | Identification of Ski as a target for Aurora A kinase | |
Document type | dc.type | Artículo de revista | |
dcterms.accessRights | dcterms.accessRights | Acceso Abierto | |
Cataloguer | uchile.catalogador | SCOPUS | |
Indexation | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI | |
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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile