Development of a scalable procedure by a discontinuous crossflow DF/UF to obtain a concentrate of chenopodin from a dead-end centrifugal UF at bench scale
Author
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Arazo, Migdalia
Author
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Jaque, Néstor
Author
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Caro, Nelson
Author
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Abugoch James, Lilian
Author
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Tapia Villanueva, Cristian
Admission date
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2020-05-13T16:38:05Z
Available date
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2020-05-13T16:38:05Z
Publication date
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2020
Cita de ítem
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Food Chemistry 313 (2020) 126154
es_ES
Identifier
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10.1016/j.foodchem.2019.126154
Identifier
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https://repositorio.uchile.cl/handle/2250/174691
Abstract
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The aim of this study was to develop a scalable crossflow diafiltration/ultrafiltration procedure for quinoa 11S globulin purification starting at the bench scale using Ultra15 centrifugal filter devices. The electrophoretic profiles of centrifugal ultrafiltration fractions showed a high heterogeneity in the bands, while crossflow ultrafiltration reduced the phenomena of protein sticking to the membrane, avoiding aggregate formation. In the crossflow protein concentration, flux decline curves were studied according to Hermia's fouling mechanisms and the resistance in a series model. High reversible resistance was related to external mechanisms due to complete blockage of the membrane surface followed by cake formation. The crossflow ultrafiltration was the most efficient technique for obtaining 57 kDa chenopodin isolate with higher processing capacity, purity and protein yield. The diafiltration/ultrafiltration process proved to be adequate and easy to handle to scale up the production of the 11S quinoa globulin.
es_ES
Patrocinador
dc.description.sponsorship
Comisión Nacional de Investigación Científica y Tecnológica 21150356
Corporación de Fomento de la Producción INNOVA CORFO 12IDL2-13621
Universidad de Chile (Project PEEI. 2nd version 2017)
Development of a scalable procedure by a discontinuous crossflow DF/UF to obtain a concentrate of chenopodin from a dead-end centrifugal UF at bench scale