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Authordc.contributor.authorPiñuñuri Flores, Raúl 
Authordc.contributor.authorCastaño Moreno, Erika 
Authordc.contributor.authorLlanos Silva, Miguel 
Authordc.contributor.authorRonco Macchiavello, Ana María 
Admission datedc.date.accessioned2020-06-09T21:08:50Z
Available datedc.date.available2020-06-09T21:08:50Z
Publication datedc.date.issued2020
Cita de ítemdc.identifier.citationPlacenta 94 (2020) 20–25es_ES
Identifierdc.identifier.other10.1016/j.placenta.2020.03.009
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/175351
Abstractdc.description.abstractIntroduction: Folates are essential nutrients for fetal development and pregnancy outcomes; they are transported to the fetus during gestation through specific folate transporters located in the placenta. In preterm newborns, we previously showed a lower placental mRNA expression of FOLR1 along with higher folate and lower vitamin B12 cord blood levels. Thereby we aimed to explore FOLR1 methylation in placentas of preterm newborns and hypothesized an increased FOLR1 methylation associated with cord blood folates and vitamin B12 concentrations. Methods: FOLR1 methylation and mRNA were determined by methylation sensitive-high resolution melting (MS-HRM) and by real-time PCR respectively, in two placental sides of placental tissues: maternal (basal, BP) and fetal plates (chorionic, CP) of moderate preterm infants (32-36 gestational age) and term birth (37-41 gestational weeks). Folates and vitamin B12 were determined by electrochemiluminescence in umbilical cord blood samples from term and preterm newborns. Results: We found that in preterm newborns, FOLR1 mRNA was lower in both plates of placenta compared with term newborns (p < 0,05) and was negatively associated with methylation of FOLR1 in CP. Preterm newborns presented higher folate and lower vitB12 concentrations in cord blood which correlated with increased placental FOLR1 methylation. Discussion: In preterm newborns, placental FOLR1 expression is regulated by epigenetic mechanisms and presumably by maternal concentrations of folate and vitamin B12.es_ES
Patrocinadordc.description.sponsorshipomision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1130188 CONICYT-PCHA/Magister Nacional 2016-22150940es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherElsevieres_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourcePlacentaes_ES
Keywordsdc.subjectFOLR1 mRNA and methylationes_ES
Keywordsdc.subjectPlacentaes_ES
Keywordsdc.subjectPreterm newbornes_ES
Keywordsdc.subjectFolateses_ES
Keywordsdc.subjectVitamin B12es_ES
Keywordsdc.subjectMS-HRMes_ES
Títulodc.titleEpigenetic regulation of folate receptor-alpha (FOLR1) in human placenta of preterm newbornses_ES
Document typedc.typeArtículo de revistaes_ES
Catalogueruchile.catalogadorapces_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile