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Detección de virus del síndrome reproductivo y respiratorio porcino (PRRS) en fómites, vectores y aire en dos granjas intensivas de cerdos de la Región Metropolitana de Chile
(Universidad de Chile, 2022)
El Síndrome del virus reproductivo y respiratorio porcino (PRRSV), es una enfermedad que afecta a los porcinos de todas las edades causando pérdidas económicas en los planteles de producción. La transmisión del virus puede generarse por varias vías...
The Reproductive and respiratory virus syndrome (PRRSV) is a disease that affects pigs of all ages, causing economical losses in productions facilities. PRRSV can be transmitted by various ways including airborne particles, flies and fomites. Chile declared itself disease free to the OIE in 2012 however in a new strain was detected in the country in 2013 and currently an eradication program is being carried out and it is mandatory to report it to the SAG (Servicio Agrícola y Ganadero). To evaluate the presence of this virus in environmental samples (air, flies and fomites) and the control simple (oral fluid) in 2 facilities (Plantel A y Plantel B) in the Metropolitan Region that had positive cases in the summer of 2019, which were analyzed by real-time PCR and considered positive at Ct <40. In the Plantel A, a 69% of the oral fluid samples, 25% of the air samples inside the hall, a 25% of the fence and floor fomites and a 75% of feeder and door fomites, were positive. Respect to the Plantel B, 75% of the oral fluid samples, 20% of air outside the hall, 25% of floor fomites and 8% flies samples, were positive. To determinate the individual association between environmental samples and control samples, the Fisher Test were performed, in the majority of the cases values below P < 0.05 were reported, only fomite samples from Plantel A with a p 0.14 indicated association with the oral fluid samples, so they could be considered as a sample option to detect the presence of PRRSV....
The Reproductive and respiratory virus syndrome (PRRSV) is a disease that affects pigs of all ages, causing economical losses in productions facilities. PRRSV can be transmitted by various ways including airborne particles, flies and fomites. Chile declared itself disease free to the OIE in 2012 however in a new strain was detected in the country in 2013 and currently an eradication program is being carried out and it is mandatory to report it to the SAG (Servicio Agrícola y Ganadero). To evaluate the presence of this virus in environmental samples (air, flies and fomites) and the control simple (oral fluid) in 2 facilities (Plantel A y Plantel B) in the Metropolitan Region that had positive cases in the summer of 2019, which were analyzed by real-time PCR and considered positive at Ct <40. In the Plantel A, a 69% of the oral fluid samples, 25% of the air samples inside the hall, a 25% of the fence and floor fomites and a 75% of feeder and door fomites, were positive. Respect to the Plantel B, 75% of the oral fluid samples, 20% of air outside the hall, 25% of floor fomites and 8% flies samples, were positive. To determinate the individual association between environmental samples and control samples, the Fisher Test were performed, in the majority of the cases values below P < 0.05 were reported, only fomite samples from Plantel A with a p 0.14 indicated association with the oral fluid samples, so they could be considered as a sample option to detect the presence of PRRSV....
Síntesis y caracterización de micropartículas tioladas y sulfonatadas de quitosano para la formulación de una vacuna oral contra circovirus porcino tipo II (PCV2)
(UNIVERSIDAD DE CHILE, 2017)
funcionalizó con grupos azufrados para preparar quitosano tiolado (QT) y sulfonado (QS). Con estos quitosanos se formularon micropartículas de quitosano tioladas (MPQT) y de quitosano sulfonadas (MPQS) cargadas con el antígeno del circovirus porcino tipo II...
The use of microparticles (MPs) of natural polymers has been described as an innovative technique of formulating adjuvants for various vaccines. For this title memory chitosan (Qo) was used as the natural polymer which was functionalized with sulfur groups to prepare thiolated (QT) and sulfonated chitosan (QS). With these chitosans, thiolated chitosan microparticles (MPQT) and sulphonated chitosan microparticles (MPQS) loaded with the porcine circovirus type II (PCV2) antigen were formulated. The formulated and loaded chitosan microparticles (MPQ) were obtained by ionic gelation and spray drying techniques. It was determined the presence of sulfur groups in the MPQ by Fourier transform infrared spectroscopy (FTIR) and these MPs were characterized in terms of morphology, size and amount of charge by means of Scanning Electron Microscope (SEM) and Zeta potential, besides evaluating such parameters such as encapsulation efficiency (EE) and yields of production (R), the presence of antigen was also evaluated by Dot Blot and finally in vitro mucoadhesivity studies were performed. The results obtained by FTIR indicate the occurrence of new absorption peaks that coincide with the groups -SH, with C-O-S bonds and for S = O groups. By SEM the formation of MPs of Qo with more or less spherical shapes, defined edges, smooth to rough surface, and sizes varying between 1 and 50 μm are observed. By means of zeta potential the MPQC and MPQT presented positive net load while the MPQS presented negative net load. All MPs have EE values higher than 80%, R about 30% for 1X MPs and are positive for the presence of PCV2 Cap antigen at Dot Blot analysis. Finally, mucoadhesivity assays show that MPQT and MPQS had better mucoadhesivity with respect to MPQC. In conclusion, it is possible to prepare and characterize Qo functionalized with thiol and sulfonate groups, to formulate three types of Qo MPs loaded with PCV2 Cap antigen and to characterize them in their entirety....
The use of microparticles (MPs) of natural polymers has been described as an innovative technique of formulating adjuvants for various vaccines. For this title memory chitosan (Qo) was used as the natural polymer which was functionalized with sulfur groups to prepare thiolated (QT) and sulfonated chitosan (QS). With these chitosans, thiolated chitosan microparticles (MPQT) and sulphonated chitosan microparticles (MPQS) loaded with the porcine circovirus type II (PCV2) antigen were formulated. The formulated and loaded chitosan microparticles (MPQ) were obtained by ionic gelation and spray drying techniques. It was determined the presence of sulfur groups in the MPQ by Fourier transform infrared spectroscopy (FTIR) and these MPs were characterized in terms of morphology, size and amount of charge by means of Scanning Electron Microscope (SEM) and Zeta potential, besides evaluating such parameters such as encapsulation efficiency (EE) and yields of production (R), the presence of antigen was also evaluated by Dot Blot and finally in vitro mucoadhesivity studies were performed. The results obtained by FTIR indicate the occurrence of new absorption peaks that coincide with the groups -SH, with C-O-S bonds and for S = O groups. By SEM the formation of MPs of Qo with more or less spherical shapes, defined edges, smooth to rough surface, and sizes varying between 1 and 50 μm are observed. By means of zeta potential the MPQC and MPQT presented positive net load while the MPQS presented negative net load. All MPs have EE values higher than 80%, R about 30% for 1X MPs and are positive for the presence of PCV2 Cap antigen at Dot Blot analysis. Finally, mucoadhesivity assays show that MPQT and MPQS had better mucoadhesivity with respect to MPQC. In conclusion, it is possible to prepare and characterize Qo functionalized with thiol and sulfonate groups, to formulate three types of Qo MPs loaded with PCV2 Cap antigen and to characterize them in their entirety....
Implementation of a porcine liver transplant model in chile Implementación de un modelo porcino de trasplante hepático en chile
(Sociedad de Cirujanos de Chile, 2020)
Pulpa de paja como alternativa material y su proyección de diseño como superficie en planteles porcinos de recría
(Universidad de Chile, 2012)
Circovirus porcino: un virus pequeño que genera un gran problema
(Universidad de Chile, Facultad de Ciencias Veterinarias y Pecuarias, 2007-12)
CIRCOVIRUS PORCINO: UN VIRUS PEQUEñO QUE GENERA
UN GRAN PROBLEMA
PORCINE CIRCOVIRUS: A SMALL VIRUS THAT CAUSES A GREAT PROBLEM
Jorge noriegA1, PAUlinA reyes1, sergio bUcArey1
AbstrAct
Porcine circoviruses type 1...
UMen Los circovirus porcinos tipo 1 y tipo 2 (PCV-1 y PCV-2) son virus muy pequeños (17 nm), no envueltos, de simetría icosahédrica (Tisher y col., 1982), con un DNA circular de hebra simple de aproximadamente 1.700 nucleótidos de longitud, el cual...
UMen Los circovirus porcinos tipo 1 y tipo 2 (PCV-1 y PCV-2) son virus muy pequeños (17 nm), no envueltos, de simetría icosahédrica (Tisher y col., 1982), con un DNA circular de hebra simple de aproximadamente 1.700 nucleótidos de longitud, el cual...
Descripción temporal y espacial del ganado porcino, ovino, caprino y equino en ferias y plantas faenadoras de carne en Chile
(Universidad de Chile, 2004)
UNIVERSIDAD DE CHILE
FACULTAD DE CIENCIAS VETERINARIAS Y PECUARIAS
ESCUELA DE CIENCIAS VETERINARIAS
DESCRIPCIÓN TEMPORAL Y ESPACIAL DEL GANADO
PORCINO, OVINO, CAPRINO Y EQUINO EN FERIAS Y...
UNIVERSIDAD DE CHILE FACULTAD DE CIENCIAS VETERINARIAS Y PECUARIAS ESCUELA DE CIENCIAS VETERINARIAS DESCRIPCIÓN TEMPORAL Y ESPACIAL DEL GANADO PORCINO, OVINO, CAPRINO Y EQUINO EN FERIAS Y PLANTAS FAENADORAS DE CARNE EN CHILE LUZ MARÍA...
UNIVERSIDAD DE CHILE FACULTAD DE CIENCIAS VETERINARIAS Y PECUARIAS ESCUELA DE CIENCIAS VETERINARIAS DESCRIPCIÓN TEMPORAL Y ESPACIAL DEL GANADO PORCINO, OVINO, CAPRINO Y EQUINO EN FERIAS Y PLANTAS FAENADORAS DE CARNE EN CHILE LUZ MARÍA...
Efecto en la carga viral y seroconversión en cerdos inmunizados con dos formulaciones distintas de vacunas contra circovirus porcino tipo 2
(Universidad de Chile, 2017)
EFECTO EN LA CARGA VIRAL Y SEROCONVERSIÓN EN
CERDOS INMUNIZADOS CON DOS FORMULACIONES
DISTINTAS DE VACUNAS CONTRA CIRCOVIRUS
PORCINO TIPO 2
UNIVERSIDAD DE CHILE
FACULTAD DE CIENCIAS VETERINARIAS Y PECUARIAS...
laboratorio quienes contribuyen mucho con mi trabajo. Quiero agradecer particularmente a don Toribio Correa, quien permitió poder llevar a cabo esta investigación en su plantel porcino, “Comercial e Industrial el Monte”. Agradezco a mi familia, en...
laboratorio quienes contribuyen mucho con mi trabajo. Quiero agradecer particularmente a don Toribio Correa, quien permitió poder llevar a cabo esta investigación en su plantel porcino, “Comercial e Industrial el Monte”. Agradezco a mi familia, en...
Efecto en los parámetros productivos de cerdos vacunados de manera oral contra circovirus porcino tipo 2
(Universidad de Chile, 2014)
de desmedro multisistémico post destete (PMWS), este síndrome es multifactorial y uno de sus causantes más importante es el circovirus porcino tipo II (PCV-2), el cual se encuentra en cerca del 100% de los planteles productivos de cerdos a nivel...
Evaluación de fracciones antigénicas, enzimáticas de Fasciola hepatica en el diagnóstico inmunológico de fasciolosis en ovinos, equinos y porcinos
(Universidad de Chile, 2007)
INMUNOLOGICO DE FASCIOLOSIS EN
OVINOS, EQUINOS Y PORCINOS.
NORMAN EDGARDO AGUILERA QUEVEDO
PROFESOR GUIA: Dra. TEXIA GORMAN GOFFRERI
Financiado por Proyecto FONDECYT 194-0245
Proyecto de Enlace DID E005...
infección en ovinos, respectivamente. La sensibilidad y especificidad de la fracción de <30 kDa en el inmunodiagnóstico de la fasciolosis en porcinos y equinos también fueron elevadas (valores entre 80% y 90%). 5 ABSTRACT Fasciolosis...
infección en ovinos, respectivamente. La sensibilidad y especificidad de la fracción de <30 kDa en el inmunodiagnóstico de la fasciolosis en porcinos y equinos también fueron elevadas (valores entre 80% y 90%). 5 ABSTRACT Fasciolosis...
Comparación del patrón de seroconversión contra circovirus porcino tipo 2 (PCV-2) entre cerdos vacunados y cerdos infectados naturalmente
(Universidad de Chile, 2012)
UNIVERSIDAD DE CHILE
FACULTAD DE CIENCIAS VETERINARIAS Y PECUARIAS
ESCUELA DE CIENCIAS VETERINARIAS
COMPARACIÓN DEL PATRÓN DE SEROCONVERSIÓN CONTRA
CIRCOVIRUS PORCINO TIPO 2 (PCV-2) ENTRE CERDOS...
PORCINO TIPO 2 (PCV-2) ENTRE CERDOS VACUNADOS Y CERDOS INFECTADOS NATURALMENTE IZVARA ODETTE ALDUNCE FORTTES NOTA FINAL: ………………… NOTA FIRMA PROFESOR GUÍA : SERGIO...
PORCINO TIPO 2 (PCV-2) ENTRE CERDOS VACUNADOS Y CERDOS INFECTADOS NATURALMENTE IZVARA ODETTE ALDUNCE FORTTES NOTA FINAL: ………………… NOTA FIRMA PROFESOR GUÍA : SERGIO...