Artículos de revistas

Peptide targeted gold nanoplatform carrying miR-145 induces antitumoral effects in ovarian cancer cells

2023-11-21T14:27:30Z

Peptide targeted gold nanoplatform carrying miR-145 induces antitumoral effects in ovarian cancer cells Salas Huenuleo, Edison Sebastián; Hernández Jorquera, Andrea Carolina; Lobos González, Lorena; Polakovicová, Iva; Morales Zavala, Francisco Andrés; Araya, Eyleen; Celis, Freddy; Romero Osses, Carmen Aurora; Kogan, Marcelo Javier One of the recent attractive therapeutic approaches for cancer treatment is restoring downregulated microRNAs. They play an essential muti-regulatory role in cellular processes such as proliferation, differentiation, survival, apoptosis, cell cycle, angiogenesis, and metastasis, among others. In this study, a gold nanoplatform (GNPF) carrying miR-145, a downregulated microRNA in many cancer types, including epithelial ovarian cancer, was designed and synthesized. For targeting purposes, the GNPF was functionalized with the FSH33 peptide, which provided selectivity for ovarian cancer, and loaded with the miR-145 to obtain the nanosystem GNPF-miR-145. The GNPF-mir-145 was selectively incorporated in A2780 and SKOV3 cells and significantly inhibited cell viability and migration and exhibited proliferative and anchor-independent growth capacities. Moreover, it diminished VEGF release and reduced the spheroid size of ovarian cancer through the damage of cell membranes, thus decreasing cell viability and possibly activating apoptosis. These results provide important advances in developing miR-based therapies using nanoparticles as selective vectors and provide approaches for in vivo evaluation.

Psychosocial factors and sociodemographic characteristics associated with suicidality risk in chilean adolescents

2023-08-22T21:15:30Z

Psychosocial factors and sociodemographic characteristics associated with suicidality risk in chilean adolescents Zulic Agramunt, Christianne Milena; Guzmán Guzmán, Iris Paola; Delgado Floody, Pedro Antonio; Cerda Saavedra, Monserrat Belén; Gutiérrez de la Fuente, Patricio; Meza Solano, Mario Patricio; Sagredo Berríos, Claudia; Pérez Testor, Carles Background: Suicidality in adolescents is a growing concern and is currently a public health issue in Chile and the world. Objective: To determine the association between the risk of suicidality with self-harm, sociodemographic parameters (that is, gender and type of school), psychosocial variables, and social and family support in Chilean adolescents. Methods: In a cross-sectional study, 829 (377, 45.5% girls) children/adolescents between 10 and 19 years of age participated. Suicidality, self-esteem, health-related quality of life (HRQoL), and social support perception were evaluated by standard, validated questionnaires. The presence of self-harm, dating violence, and family dysfunction was also evaluated through a self-report survey. Results: Suicidality risk was related to low self-esteem (OR = 9.73; 95%; CI = 6.62–14.28; p < 0.001), low HRQoL (OR = 5.0; 95%; CI = 3.51–7.13; p < 0.001), low social support (OR; 3.38, 95%; CI; 2.48–4.6; p < 0.001), and self-harm (OR = 8.03; 95%; CI = 5.69–11.33; p < 0.001). In family terms, suicidality risk was associated with exposure to physical (OR = 2.47, 95%CI; 1.69–3.6; p < 0.001) and psychological (OR = 1.78, 95; 1.33–2.39; p < 0.001) aggression between parents, and with considering their family dysfunctional (OR = 2.41 95%; CI = 1.69–3.41; p < 0.001). Finally, suicidality was associated with feeling mistreated by a boyfriend/girlfriend (OR = 2.18; 95% CI = 1.19–3.98; p = 0.011). Conclusion: Suicidality was associated with self-harm, low social, psychological and family well-being, and/or feeling mistreated by a boyfriend/girlfriend.

Estudio comparativo de la calidad biofarmacéutica de Diazepam 10 mg comercializados en el mercado peruano

2023-07-23T21:03:28Z

Estudio comparativo de la calidad biofarmacéutica de Diazepam 10 mg comercializados en el mercado peruano Saavedra Suárez, Segundo; Alva Plasencia, Pedro; Gutiérrez Ramos, Miriam; Curo Vallejos, Yuri; Rodríguez Saavedra, Lennin Roswell Introduction: diazepam belongs to a group of medicines called benzodiazepines. These drugs are known to work in the brain through GABA and, it used to relieve anxiety and control the agitation caused by alcohol withdrawal. Method: variation in weight, friability, hardness, disintegration time, quantification of active ingredient, speed and dissolution profile between the innovative and multi-source drugs marketed in the Peruvian market. The evaluation of these presentations did with the USP 42 Pharmacopeia. Results: the results established that all Diazepam 10 mg multi-source tablets included in this research are bioequivalent with the chosen innovative brand and, therefore, can be interchangeable. Conclusions: The results observed that the multi-source tablets of Diazepam 10 mg included in this research are bioequivalent with the innovator and, therefore, allow the determination of pharmaceutical equivalence as a support element in the decision-making process for purchasing in the service pharmacist.; Resumen Introducción: el Diazepam pertenece a un grupo de medicamentos llamados benzodiazepinas. Se sabe que estos medicamentos actúan en el cerebro a través del GABA. Se utiliza para aliviar la ansiedad y control de la agitación causada por la abstinencia de alcohol. Método: se evaluó la variación de peso, friabilidad, dureza, tiempo de desintegración, cuantificación de principio activo, velocidad y perfil de disolución entre el medicamento innovador y medicamentos multifuentes comercializados en el mercado peruano, según lo establecido en la USP 42. Resultados: los resultados permitieron establecer que todas las tabletas multifuente de Diazepam 10 mg incluidos en esta investigación son bioequivalentes con la marca innovadora elegida y, por lo tanto, pueden ser intercambiables. Conclusiones: se estableció que las tabletas multifuentes de Diazepam 10 mg incluidos en esta investigación son bioequivalentes con el innovador y, por lo que permite proponer a la comunidad científica la determinación de la equivalencia farmacéutica como elemento de apoyo en la toma de decisiones de compra en el servicio farmacéutico.

Un análisis bibliométrico del concepto “novel foods” en publicaciones de investigación entre 1997 y 2020

2023-07-21T17:22:02Z

Un análisis bibliométrico del concepto “novel foods” en publicaciones de investigación entre 1997 y 2020 Puente Díaz, Luis Andrés; Doblado Mendoza, Andrea Lizeth; Ortiz Estévez, Adriana Marcela; Castillo Benites, Darwin Rafael "Novel Foods" are any foods produced with new technologies and substances, as well as those that have not been used for human consumption to a significant degree. This article presents a general review of publications related to "Novel Foods", through a bibliometric analysis using information extracted from Web of Science (WoS) between 1997 and 2020. The review identified the most cited and productive authors, as well as the main countries and journals publishing. The results are presented in tables and graphs, and a bibliometric mapping using the VOSviewer program is also included. The countries with the highest number of publications and citations were the United States, England, and Netherlands; while among the most productive authors were Naska, A. (Greece), Siani, A. (Italy) and Turck, D. (France). This work may provide guidance to academics who wish to conduct research in this field together with other researchers from different countries and institutions.; En Europa se consideran como “Novel Foods” cualquier alimento producido con nuevas tecnologías y sustancias, así como los que no se hayan utilizado para el consumo humano en un grado significativo dentro de la UE antes del 15 de mayo de 1997. Este artículo presenta una revisión general de las publicaciones relacionadas con Novel Foods, mediante un análisis bibliométrico utilizando información extraída de Web of Science (WoS) entre 1997 y 2020. La revisión identificó a los autores más citados y productivos, así como a los principales países y revistas que realizan publicaciones. Los resultados se exponen en tablas y gráficos, además se incluye un mapeo bibliométrico obtenido con el programa VOSviewer. Entre los países con mayor número de publicaciones y citaciones se encontró Estados Unidos, Inglaterra y Países Bajos; mientras que entre los autores más productivos se encontró a Naska A. (Grecia), Siani A. (Italia) y Turck D. (Francia). Este trabajo puede orientar a diversos académicos que deseen realizar investigaciones en este campo junto con otros investigadores de diferentes países e instituciones