Browsing by Author "141de15c-27fd-4465-b182-7c78deca47b3"
Now showing items 1-6 of 6
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Nova, Esteban; Montecinos, Felipe; Brunet, Juan E.; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio (2007)FtsZ (Filamentous temperature sensitivity Z) cell division protein from Escherichia coli binds the fluorescence probe DAPI. Bundling of FtsZ was facilitated in the presence of DAPI, and the polymers in solution remained ...
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Arbildua, José J.; Brunet, Juan E.; Jameson, David M.; López, Maribel; Nova, Esteban; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio (2006)The goal of this work was to determine the binding properties and location of 4′,6-diamidino-2-phenylindole (DAPI) complexed with tubulin. Using fluorescence anisotropy, a dissociation constant of 5.2 ± 0.4 μM for the ...
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Cruz Vargas, Victor Ignacio; Brunet, Juan E.; Jameson, David M. (1991)The quenching by molecular oxygen of the fluorescence of several probes complexed to apohorseradish peroxidase has been studied by intensity and time-resolved fluorescence methods. The probes utilized include 1-anilino-8 ...
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Montecinos Franjola, Felipe Antonio; James, Nicholas G.; Concha Marambio, Luis; Brunet, Juan E.; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio; Jameson, David M. (Elsevier, 2014)Cell division protein FtsZ cooperatively self-assembles into straight filaments when bound to GTP. A set of conformational changes that are linked to FtsZ GTPase activity are involved in the transition from straight to ...
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Lasagna, Mauricio; Cruz Vargas, Victor Ignacio; Jameson, David M.; Brunet, Juan E. (1996)The environmentally sensitive fluorescent probes 6-propionyl-2-(N,N- dimethylamino)naphthalene (PRODAN) and 2'-(N,N-dimethylamino)-6-naphthoyl-4- trans-cyclohexanioc acid (DANCA) form complexes with the heme binding site ...
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Sánchez, Susana A.; Brunet, Juan E.; Jameson, David M.; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio (2004)The pathway for the in vitro equilibrium unfolding of the tubulin heterodimer by guanidinium chloride (GdmCl) has been studied using several spectroscopic techniques, specifically circular dichroism (CD), two-photon ...