Browsing by Author "2df97d37-f282-45fa-9ae1-837ba8d08761"

Now showing items 1-9 of 9

    • ADP-dependent kinases from the archaeal order methanosarcinales adapt to salt by a non-canonical evolutionarily ... 
      González Órdenes, Felipe; Cea, Pablo A.; Fuentes, Nicolás; Muñoz, Sebastián M.; Zamora, Ricardo A.; Leonardo, Diego; Garratt, Richard C.; Castro Fernández, Víctor; Guixé Leguía, Victoria Cristina (Frontiers Media SA., 2018-06-26)
      Halophilic organisms inhabit hypersaline environments where the extreme ionic conditions and osmotic pressure have driven the evolution of molecular adaptation mechanisms. Understanding such mechanisms is limited by the ...
    • Characterization of hydroxymethylpyrimidine phosphate kinase from mesophilic and thermophilic bacteria and ... 
      Cea, Pablo A.; Araya, Gissela; Vallejos Baccelliere, Gabriel; Recabarren, Rodrigo; Alzate Morales, Jans; Babul Cattan, Jorge; Guixé Leguía, Victoria; Castro Fernández, Víctor (Elsevier, 2020)
      The hydroxymethylpyrimidine phosphate kinases (HMPPK) encoded by the thiD gene are involved in the thiamine biosynthesis pathway, can perform two consecutive phosphorylations of 4-amino-5-hydroxymethyl-2methyl pyrimidine ...
    • Cold adapted ADP-dependent sugar kinase: biophysical and evolutionary study of its flexibility 
      Guixé Leguía, Victoria Cristina; Zamorano Sarria, Ricardo; Ramírez Sarmiento, César A.; Castro Fernández, Víctor; Cayul Villalobos, Pablo; Komives, Elizabeth (Wiley, 2017)
    • Evolution, metabolism and molecular mechanisms underlying extreme adaptation of euryarchaeota and its ... 
      Castro Fernández, Víctor; Zamora, Ricardo; Herrera Morandé, Alejandra; Vallejos, Gabriel; González Órdenes, Felipe; Guixé Leguía, Victoria Cristina (IntechOpen, 2017)
      Archaeal organisms harbor many unique genotypic and phenotypic properties, testifying their peculiar evolutionary status. Thus, the so‐called extremophiles must be adequately adapted to cope with many extreme environments ...
    • Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity ... 
      Castro Fernández, Víctor; Herrera Morande, Alejandra; Zamorano Sarria, Ricardo; Merino, Felipe; González Órdenes, Felipe; Padilla Salinas, Felipe; Pereira, Humberto M.; Brandao Neto, José; Garratt, Richard C.; Guixé Leguía, Victoria Cristina (American Society for Biochemistry and Molecular Biology, 2017)
      One central goal in molecular evolution is to pinpoint the mechanisms and evolutionary forces that cause an enzyme to change its substrate specificity; however, these processes remain largely unexplored. Using the glycolytic ...
    • Single-nucleotide polymorphisms (snp) mining and their effect on the tridimensional protein structure ... 
      Vallejos Vidal, Eva; Reyes Cerpa, Sebastián; Rivas Pardo, Jaime Andrés; Maisey, Kevin; Yáñez López, José; Valenzuela, Héctor; Cea, Pablo A.; Castro Fernández, Víctor; Tort, Lluis; Sandino, Ana M.; Imarai, Mónica; Reyes López, Felipe E. (Frontiers Media, 2020)
      Single-nucleotide polymorphisms (SNPs) are single genetic code variations considered one of the most common forms of nucleotide modifications. Such SNPs can be located in genes associated to immune response and, therefore, ...
    • Structural and functional analysis of the ASM p.Ala359Asp mutant that causes acid sphingomyelinase deficiency 
      Acuña, Mariana; Castro Fernández, Víctor; Latorre Mora, Mauricio; Castro, Juan; Schuchman, Edward H.; Guixé Leguía, Victoria Cristina; González Canales, Mauricio; Zanlungo, Silvana (Elsevier, 2016)
      Niemann-Pick disease (NPD) type A and B are recessive hereditary disorders caused by deficiency in acid sphingomyelinase (ASM). The p.A1a359Asp mutation has been described in several patients but its functional and structural ...
    • Tuning of conformational dynamics through evolution-based design modulates the catalytic adaptability of ... 
      Zamora, Ricardo A.; Ramírez Sarmiento, César; Castro Fernández, Víctor; Villalobos, Pablo; Maturana, Pablo; Herrera Morandé, Alejandra; Komives, Elizabeth A.; Guixe, Victoria (American Chemical Society, 2020)
      Conformational flexibility plays a critical role in enzyme function and is a key aspect in transitions from an open to a closed state induced by substrate binding and product release. Psychrophilic enzymes display a high ...
    • Unusual dimerization of a BcCsp mutant leads to reduced conformational dynamics 
      Carvajal, Alonso I.; Vallejos, Gabriel; Komives, Elizabeth A.; Castro Fernández, Víctor; Leonardo, Diego A.; Garratt, Richard C.; Ramírez Sarmiento, César A.; Babul Cattán, Jorge (Wiley, 2017)
      Cold shock proteins (Csp) constitute a family of ubiquitous small proteins that act as RNA-chaperones to avoid cold-induced termination of translation. All members contain two subdomains composed of 2 and 3 beta-strands, ...