Browsing by Author "47527eb2-d0ae-4fc3-8753-865eb5963a7a"
Now showing items 1-12 of 12
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Niemeyer,; Ureta, Tito; Clark-Turri, Lyllian (Kluwer Academic Publishers, 1975)1. Glucokinase is one of four glucose phos-phorylating enzymes present in rat liver. Its distinctive features are a high Km for glucose (high-Km isozyme) and a rather narrow substrate specificity. In contrast, the other ...
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Ureta Aravena, Tito; Radojković, Jasna; Díaz Pérez, Nelson; Slebe Tajmuch, Juan Carlos; Lozano, Carlos (1978)Glucose phosphorylating activities were measured in liver extracts from two urodeles and twenty-six anurans. Fractionation on diethylaminoethyl-cellulose columns of liver extracts from these amphibians permitted the ...
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Cárdenas, María Luz; Cornish-Bowden, Athel; Ureta, Tito (1998)
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Báez, Mauricio; Preller, Ana; Ureta, Tito (Academic Press Inc., 2003)Frog oocyte glycogen synthase properties differ significantly under in vitro or in vivo conditions. The Kmapp for UDP-glucose in vivo was 1.4mM (in the presence or absence of glucose-6-P). The in vitro value was 6mM and ...
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Preller, Ana; Guixé Leguía, Victoria Cristina; Kessi, Eduardo; Ureta, Tito (1995)The characterization of fructose-1, 6-bisphosphatase in stage VI oocytes from the frog Caudiverbera caudiverbera, as well as the in vivo activity, is reported. The enzyme has a subunit molecular weight of approximately 43, ...
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Preller Simmons, Ana; Kessi Campos, Elías; Ureta, Tito (Elsevier B.V., 2007-01-22)Abstract Besides the classic direct route, frog oocytes incorporate glucosyl units into glycogen by the so-called indirect pathway. The operation of both pathways depends on glucose availability. Below 0.5 mM glucose ...
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Kessi, Eduardo; Guixé Leguía, Victoria Cristina; Preller, Ana; Ureta, Tito (Portland Press Ltd, 1996)Glycogen is the main end product of glucose metabolism in amphibian oocytes. However, in the first few minutes after [U-14C]glucose microinjection most of the label is found in lactate. The burst of lactate production and ...
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Guixé Leguía, Victoria Cristina; Preller, Ana; Kessi, Eduardo; Hofer, H. Werner; Ureta, Tito (1994)It is generally accepted that in frog full-grown oocytes glycolysis is absent and that carbon metabolic flux is largely directed to glycogen synthesis. Use of an anion exchange pellicular resin for analytical resolution ...
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Preller Simmons, Ana; Wilson, Christian A. M.; Quiroga Roger, Diego; Ureta, Tito (Elsevier, 2013)Here we set out to evaluate the role of hexokinase and glycogen synthase in the control of glycogen synthesis in vivo. We used metabolic control analysis (MCA) to determine the flux control coefficient for each of the ...
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Ureta, Tito; Fernández, Waldy Y.; Centelles, Josep J.; Cascante, Marta (2000)Hexokinase and glucose-6-phosphate dehydrogenase activities were increased in Xenopus laevis oocytes by microinjection of commercial pure enzymes. The effect of increased fractional activities on glycogen synthesis or on ...
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Guixé Leguía, Victoria Cristina; Preller, Ana; Kessi, Eduardo; Ureta, Tito (Academic Press Inc., 1997)Glycogen synthesis following glucose microinjection in frog oocytes proceeds preferentially by an indirect pathway involving gluconeogenesis from triose compounds. Because of the known regulatory role of fructose-2,6-bisP ...
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Niemeyer, Hermann; Pérez, Norma; Radojkovic, Jasna; Ureta, Tito (1962)Liver phosphorylase activity was found to be proportional to the caloric intake of the animal. Rats fed for 6 or 10 days an exclusively carbohydrate diet showed a phosphorylase content of the liver similar to animals fed ...