Browsing by Author "79ca83e1-728f-48e2-8149-7002427e8237"
Now showing items 1-7 of 7
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Venegas, Juan; Solari Illescas, Aldo (1994)A nonradioactive method is developed to detect DNA polymerase activity after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis containing gapped DNA as template. The technique is based on the use of digoxigenin- ...
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Venegas, Juan; Coñoepan, William; Pichuantes, Sergio; Miranda, Sandra; Apt Baruch, Werner; Arribada, Arturo; Zulantay Alfaro, Inés; Coronado, Ximena; Rodriguez, Jorge; Reyes, Eduardo; Solari Illescas, Aldo; Sanchez, Gittith (2009)PCR and Southern blot hybridization were used to determine the distribution of Trypanosoma cruzi clones in 37 chronic chagasic cardiopathic and non-cardiopathic patients. Parasite DNA amplified from peripheral blood or ...
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Wallace, Anne; Sanchez, Gittith; Venegas, Juan; Solari Illescas, Aldo (1995)Immune sera from mice infected with specific Zymodemes of Trypanosoma cruzi parasites displayed preferential in vitro complement-mediated trypanolytic activity with homologous or genetically related bloodstream trypomastigotes. ...
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Zulantay Alfaro, Inés; Venegas, Juan; Apt Baruch, Werner; Solari Illescas, Aldo; Sanchez, Gittith (American Society of Tropical Medicine and Hygiene, 1998)An antibody-dependent, complement-mediated lysis test performed with three Chilean trypomastigote strains used as target cells revealed strain dependence in the detection of lytic antibodies in chronic chagasic sera. The ...
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Venegas, Juan; Miranda, Sandra; Coñoepan, William; Pîchuantes, Sergio; Jercic, María Isabel; González, Christian; Gajardo, Marta; Apt Baruch, Werner; Arribada, Arturo; Sánchez, Gittith (2010)To investigate whether Trypanosoma cruzi populations found in chagasic cardiopathic and non-cardiopathic patients are genetically differentiated, three molecular microsatellite markers were analysed. This analysis was also ...
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Venegas, Juan; Coñoepan, William; Pichuantes, Sergio; Miranda, Sandra; Jercic, María Isabel; Gajardo, Marta; Sánchez, Gittith (2009)To better understand the evolution of the etiologic agent of Chagas disease, we cloned and sequenced 25 alleles from five Tripanosoma cruzi microsatellite markers. The study of the sequences showed highly conserved alleles ...
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Venegas, Juan; Solari Illescas, Aldo (1995)A DNA polymerase was purified to near homogeneity from Trypanosoma cruzi epimastigotes. This preparation had a major polypeptide of 50 kDa and a minor band of 45 kDa. SDS-PAGE studies and a novel colorimetric activity gel ...