Browsing by Author "8314e15d-4a39-46b1-ae5c-fc59ad807d89"

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    • Activity of Recombinant α and β Subunits of Casein Kinase II from Xenopus laevis 
      Hinrichs, María V.; Jedlicki, Ana; Tellez, Rowena; Pongor, Sándor; Gatica, Marta; Allende, Catherine C.; Allende, Jorge E. (1993)
      Casein kinase II (CKII) is a ubiquitous protein kinase, found predominantly in cell nuclei, which has two subunits in a tetrameric α2β2 or αα′2 conformation. The catalytic center is present in the α subunit which is active ...
    • Aminoacyl transfer from phenylalanyl-tRNA microinjected into xenopus laevis oocytes 
      Gatica, Marta; Allende, Jorge E. (1977)
      Xenopus laevis oocytes were injected with [14C] phe-tRNA and the fate of the aminoacyl moiety was studied. The radioactive phenylalanine is gradually hydrolized off the tRNA once inside the cell. The rate of deacylation ...
    • DNA Inhibits the Catalytic Activity of the α Subunit of Protein Kinase CK2 
      Gatica, Marta; Jacob, Germaine; Allende, Jorge E.; Allende, Catherine C. (1995)
      The recombinant α subunit of protein kinase CK2 (casein kinase 2) from Xenopus laevis is inhibited by the addition of single stranded or double stranded DNA. This inhibition is competitive with the casein substrate, having ...
    • In vivo repair of the 3′ terminus of transfer RNA injected into amphibian oocytes 
      Solari Illescas, Aldo; Gatica, Marta; Allende, Jorge E. (1977)
      Yeast transfer RNA specific for phenylalanine has been treated chemically to remove either one or two nucleotides of its 3′ terminus and has been injected into Xenopus laevis oocytes to test whether this RNA can be repaired ...
    • Isolation of threonyl adenylate-enzyme complex 
      Allende, Jorge E.; Allende, Catherine C.; Gatica, Marta; Matamala, María (1964)
      The aminoacyl-RNA synthetases have been shown to carry out the following two-step reaction: 1. 1) amino acid + ATP + enzyme ⇌ aminoacyl-AMP-enzyme + PPi 2. 2) aminoacyl-AMP-enzyme + sRNA ⇌ aminoacyl-sRNA + AMP + enzyme. ...
    • Nucleic acids can regulate the activity of casein kinase II 
      Gatica, Marta; Allende, Catherine C.; Allende, Jorge E. (1989)
      Casein kinase II purified from nuclei of Xenopus laevis oocytes is inhibited by several specific nucleic acids. This kinase, the main phosphorylating activity of the oocyte nucleus, is markedly inhibited by poly U at 10 ...