Now showing items 1-20 of 30

    • Segura Aguilar, Juan; Muñoz, Patricia; Paris Pizarro, Irmgard (Bentham Science, 2016)
      The pharmacological treatment of Parkinson's disease (PD) is limited to dopamine agonists and anti-cholinergic drugs that do not stop the progress of disease. L-Dopa was introduced to the treatment in 1967; this drug is ...
    • Araujo, Fillipe M. de; Ferreir, Rafael S.; Souza, Cleide S.; Santos, Cleonice Creusa dos; Rodrigues, Tácio L. R. S.; Silva, Juliana Helena C. E.; Gasparotto, Juciano; Gelain, Daniel Pens; El-Bachá, Ramón S.; Costa, María de Fátima D.; Fonseca, José Claudio M.; Segura Aguilar, Juan; Costa, Silvia L.; Silva, Víctor Diógenes A. (Elsevier, 2018-05)
      Recent evidence shows that aminochrome induces glial activation related to neuroinflammation. This dopamine derived molecule induces formation and stabilization of alpha-synuclein oligomers, mitochondria dysfunction, ...
    • Paris Pizarro, Irmgard; Pérez Pastene, Carolina; Cárdenas, Sergio; Iturra, Patricio; Muñoz, Patricia; Couve, Eduardo; Caviedes Fernández, Pablo; Segura Aguilar, Juan (SPRINGER, 2010-01-20)
      Abstract In previous studies, we observed that cells treated with aminochrome obtained by oxidizing dopamine with oxidizing agents dramatically changed cell morphology, thus posing the question if such morphological changes ...
    • Herrera, Andrea; Muñoz Tapia, Patricia; Paris Pizarro, Irmgard; Díaz Véliz, Gabriela; Mora Gutiérrez, Sergio; Inzunza, José; Hultenby, Kjell; Cárdenas Matus, Julio; Jaña Prado, Fabián; Raisman Vozari, Rita; Gysling, Katia; Abarca, Jorge; Steinbusch, Harry; Segura Aguilar, Juan (Springer, 2016)
      L-Dopa continues to be the gold drug in Parkinson's disease ( PD) treatment from 1967. The failure to translate successful results from preclinical to clinical studies can be explained by the use of preclinical models which ...
    • Santos, Cleonice C.; Araújo, Fillipe M.; Ferreira, Rafael S.; Silva, Vanessa B.; Silva, Juliana H.C.; Grangeiro, Maria S.; Soares, Érica N.; Pereira, Érica Patricia L.; Souza, Cleide S.; Costa, Silvia L.; Segura Aguilar, Juan; Silva, Victor Diogenes (Elsevier, 2017)
      Aminochrome has been suggested as a more physiological preclinical model capable of inducing five of the six mechanisms of Parkinson's Disease (PD). Until now, there is no evidence that aminochrome induces glial activation ...
    • Pérez Pastene, Carolina; Graumann, Rebecca; Díaz Grez, Fernando; Miranda, Marcelo; Venegas Francke, Pablo; Trujillo Godoy, Osvaldo; Layson, Luis; Villagra, Roque; Matamala, José Manuel; Herrera Cisterna, Luisa; Segura Aguilar, Juan (2007)
      We have studied the association of a null mutation of Glutathione Transferase M1 (GST M1 *0/0) with Parkinson's disease (MIM 168600) in a Chilean population with a strong Amerindian genetic component. We determined the ...
    • Ramírez, Alfredo; Ziegler, Andreas; Winkler, Susen; Kottwitz, Jan; Giesen, Roland; Díaz Grez, Fernando; Miranda, Marcelo; Venegas Francke, Pablo; Trujillo Godoy, Osvaldo; Avello, Rodrigo; Behrens Pellegrino, María Isabel; Klein, Christine; Segura Aguilar, Juan; Lohmann, Katja (Elsevier, 2011-01)
    • Allen, David D.; Caviedes Codelia, Raúl; Cárdenas, Ana María; Shimahara, Takeshi; Segura Aguilar, Juan; Caviedes Fernández, Pablo (DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY, 2005)
      Cell culture is highly desirable, as it provides systems for ready, direct access and evaluation of tissues. The use of tissue culture is a valuable tool to study problems of clinical relevance, especially those related ...
    • Muñoz, Patricia; Paris Pizarro, Irmgard; Segura Aguilar, Juan (Frontiers Media, 2016-04)
    • Paris Pizarro, Irmgard; Dagnino Subiabre, Alexies; Marcelain Cubillos, Katherine; Bennett, L. B.; Caviedes Fernández, Pablo; Caviedes Codelia, Raúl; Olea Azar, Claudio; Segura Aguilar, Juan (BLACKWELL SCIENCE LTD, 2001-04)
      The mechanism of copper (Cu) neurotoxicity was studied in the RCSN-3 neuronal dopaminergic cell line, derived from substantia nigra of an adult rat. The formation of a Cu-dopamine complex was accompanied by oxidation of ...
    • Paris Pizarro, Irmgard; Martínez Alvarado, P.; Cárdenas, S.; Pérez Pastene, C.; Graumann, Rebecca; Fuentes, P.; Olea Azar, Claudio; Caviedes Fernández, Pablo; Segura Aguilar, Juan (AMER CHEMICAL SOC, 2005-03)
      We report a new and specific mechanism for iron-mediated neurotoxicity using RCHT cells, which were derived from rat hypothalamus. RCHT cells exhibit immunofluorescent-positive markers for dopamine beta-hydroxylase and the ...
    • Cárdenas Muñoz, Sergio Patricio (Universidad de ChilePrograma Cybertesis, 2008)
    • Muñoz, Patricia; Cárdenas, Sergio; Huenchuguala, Sandro; Briceño, Andrea; Couve, Eduardo; Paris Pizarro, Irmgard; Segura Aguilar, Juan (Oxford University Press, 2015)
      It was reported that aminochrome induces the formation of alpha synuclein (SNCA) oligomers during dopamine oxidation. We found that DT-diaphorase (NQO1) prevents the formation of SNCA oligomers in the presence of ...
    • Huenchuguala, Sandro; Muñoz, Patricia; Graumann, Rebecca; Paris Pizarro, Irmgard; Segura Aguilar, Juan (Elsevier, 2016)
      Astrocytes are exposed to aminochrome via the oxidation of dopamine that is taken up from the synaptic cleft after its release from dopaminergic neurons. Glutathione transferase M2-2 (GSTM2) has been shown to protect ...
    • Zavala Figueroa, Patricio (Universidad de Chile, 2008)
      El objetivo de este trabajo fue demostrar que la glutatión transferasa M2-2 humana (GST M2-2), protege a los astrocitos de los efectos tóxicos del aminocromo, ya que en estudios in vitro utilizando la enzima ...
    • Klionsky, Daniel J.; Troncoso Cotal, Rodrigo; Chiong Lay, Mario; Díaz Araya, Guillermo; Glavic Maurer, Álvaro; Corbalán, Ramón; Matus, Soledad; Segura Aguilar, Juan; Hetz Flores, Claudio (Taylor & Francis inc., 2016)
    • Opazo, Patricia; Saud, Katherine; Saint Pierre, Michelle de; Cárdenas, Ana María; Allen, David D.; Segura Aguilar, Juan; Caviedes Codelia, Raúl; Caviedes Fernández, Pablo (WILEY-LISS, 2006-11-01)
      We have generated immortal neuronal cell lines from normal and trisomy 16 (Ts16) mice, a model for Down syndrome (DS). Ts16 lines overexpress DS-related genes (App, amyloid precursor protein; Sod1, Cu/Zn superoxide dismutase) ...
    • Cárdenas, Ana María; Fernández Olivares, Paola; Díaz Franulic, Ignacio; González Jamett, Arlek M.; Shimahara, Takeshi; Segura Aguilar, Juan; Caviedes Codelia, Raúl; Caviedes Fernández, Pablo (Springer, 2017)
      The Na+/myo-inositol cotransporter (SMIT1) is overexpressed in human Down syndrome (DS) and in trisomy 16 fetal mice (Ts16), an animal model of the human condition. SMIT1 overexpression determines increased levels of ...
    • Paris Pizarro, Irmgard; Martínez Alvarado, Pedro; Pérez Pastene, Carolina; Vieira, Marcelo N.N.; Olea Azar, Claudio; Raisman Vozari, Rita; Cárdenas, Sergio; Graumann, Rebecca; Caviedes Fernández, Pablo; Segura Aguilar, Juan (BLACKWELL PUBLISHING LTD, 2005-03)
      The role of dopamine in iron uptake into catecholaminergic neurons, and dopamine oxidation to aminochrome and its one-electron reduction in iron-mediated neurotoxicity, was studied in RCSN-3 cells, which express both ...
    • Segura Aguilar, Juan; Paris Pizarro, Irmgard; Muñoz, Patricia (Springer, 2016)