Browsing by Author "cd1ff8fa-c595-499c-94ec-d31ddbc0d137"

Now showing items 1-7 of 7

    • ADP-dependent kinases from the archaeal order methanosarcinales adapt to salt by a non-canonical evolutionarily ... 
      González Órdenes, Felipe; Cea, Pablo A.; Fuentes, Nicolás; Muñoz, Sebastián M.; Zamora, Ricardo A.; Leonardo, Diego; Garratt, Richard C.; Castro Fernández, Víctor; Guixé Leguía, Victoria Cristina (Frontiers Media SA., 2018-06-26)
      Halophilic organisms inhabit hypersaline environments where the extreme ionic conditions and osmotic pressure have driven the evolution of molecular adaptation mechanisms. Understanding such mechanisms is limited by the ...
    • Crystallization and preliminary crystallographic analysis of the tetrameric form of phosphofructokinase-2 ... 
      Cabrera Paucar, Ricardo; Caniuguir, Andrés; Ambrosio, Andre L. B.; Guixé Leguía, Victoria Cristina; Garratt, Richard C.; Babul Cattán, Jorge (BLACKWELL, 2006-09)
      Escherichia coli contains two phosphofructokinases, Pfk-1 and Pfk-2, which belong to unrelated protein families. In addition to catalytic function, the enzymes have converged in showing substrate inhibition by the nucleotide ...
    • Domain motions and quaternary packing of phosphofructokinase-2 from Escherichia coli studied by small angle ... 
      Cabrera Paucar, Ricardo; Fischer, Hannes; Trapani, Stefano; Craievich, Aldo F.; Garratt, Richard C.; Guixé Leguía, Victoria Cristina; Babul Cattán, Jorge (2003)
      The binding of MgATP and fructose-6-phosphate to phosphofructokinase-2 from Escherichia coli induces conformational changes that result in significant differences in the x-ray-scattering profiles compared with the unligated ...
    • Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity ... 
      Castro Fernández, Víctor; Herrera Morande, Alejandra; Zamorano Sarria, Ricardo; Merino, Felipe; González Órdenes, Felipe; Padilla Salinas, Felipe; Pereira, Humberto M.; Brandao Neto, José; Garratt, Richard C.; Guixé Leguía, Victoria Cristina (American Society for Biochemistry and Molecular Biology, 2017)
      One central goal in molecular evolution is to pinpoint the mechanisms and evolutionary forces that cause an enzyme to change its substrate specificity; however, these processes remain largely unexplored. Using the glycolytic ...
    • The Crystal Complex of Phosphofructokinase-2 of Escherichia coli with Fructose-6-phosphate. Kinetic and ... 
      Cabrera Paucar, Ricardo; Báez, Mauricio; Pereira, Humberto M.; Caniuguir, Andrés; Garratt, Richard C.; Babul Cattán, Jorge (AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2011-02-18)
      Substrate inhibition by ATP is a regulatory feature of the phosphofructokinases isoenzymes from Escherichia coli (Pfk-1 and Pfk-2). Under gluconeogenic conditions, the loss of this regulation in Pfk-2 causes substrate ...
    • The crystal complex of phosphofructokinase-2 of Escherichia coli with fructose-6-phosphate: Kinetic and ... 
      Cabrera Paucar, Ricardo; Báez, Mauricio; Pereira, Humberto M.; Caniuguir, André S.; Garratt, Richard C.; Babul Cattán, Jorge (2011)
      Substrate inhibition by ATP is a regulatory feature of the phosphofructokinases isoenzymes from Escherichia coli (Pfk-1 and Pfk-2). Under gluconeogenic conditions, the loss of this regulation in Pfk-2 causes substrate ...
    • Unusual dimerization of a BcCsp mutant leads to reduced conformational dynamics 
      Carvajal, Alonso I.; Vallejos, Gabriel; Komives, Elizabeth A.; Castro Fernández, Víctor; Leonardo, Diego A.; Garratt, Richard C.; Ramírez Sarmiento, César A.; Babul Cattán, Jorge (Wiley, 2017)
      Cold shock proteins (Csp) constitute a family of ubiquitous small proteins that act as RNA-chaperones to avoid cold-induced termination of translation. All members contain two subdomains composed of 2 and 3 beta-strands, ...