Browsing by Author "201-0335298"
Now showing items 21-40 of 50
-
Aguilera, Paulina; Marcoleta Caldera, Andrés; Lobos Ruiz, Pablo; Arranz, Rocío; Valpuesta, José M.; Monasterio Opazo, Octavio; Lagos Mónaco, Rosalba (Frontiers Media S.A., 2016)Microcin E492 (MccE492) is a pore-forming bacteriocin produced and exported by Klebsiella pneumoniae RYC492. Besides its antibacterial activity, excreted MccE492 can form amyloid fibrils in vivo as well as in vitro. It has ...
-
Benites, Julio; Valderrama, Jaime A.; Ríos, David; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio; Buc Calderón, Pedro (2016)Dihydroxynaphthyl aryl ketones 1-5 exhibit activity as tubulin polymerization inhibitors by targeting the colchicine binding site of microtubules making them potential anticancer drugs. Therefore, analogues 1-5 have been ...
-
Barriga, Gonzalo P.; Villalón Letelier, Fernando; Marquez, Chantal L.; Bignon, Eduardo A.; Acuña, Rodrigo; Ross, Breyan H.; Monasterio Opazo, Octavio; Mardones, Gonzalo A.; Vidal, Simón E.; Tischler, Nicole D. (Public Library Science, 2016)Hantaviruses can cause hantavirus pulmonary syndrome or hemorrhagic fever with renal syndrome in humans. To enter cells, hantaviruses fuse their envelope membrane with host cell membranes. Previously, we have shown that ...
-
Monasterio Opazo, Octavio; Timasheff, Serge N. (1987)The inhibitory effects of guanosine 5'-(ᵧ-fluorotriphosphate) [GTP(ᵧF)] on both the polymerization and the colchicine-dependent GTPase activity of calf brain tubulin have been studied. The results demonstrate that this ...
-
Tarragó, Adela; Monasterio Opazo, Octavio; Allende, Jorge E. (1970)The two major methionyl-tRNA species from wheat embryos and E. coli have been studied as regards their capacity to form a ternary complex with GTP and the ribosomal binding enzyme from both sources. Methionyl-tRNA1 from ...
-
Ortiz, Marco; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio (1993)The digestion of tubulin with subtilisin and the reassociation of the digestion products was followed by means of the fluorescent probe 4',6-diamidino-2-phenylindole (DAPI). The fluorescence spectra of DAPI bound to chicken ...
-
Monasterio Opazo, Octavio; Cárdenas, María Luz (2003)The kinetic mechanism of rat liver hexokinase D (‘ glucokinase’) was studied under non-co-operative conditions with 2- deoxyglucose as substrate, chosen to avoid uncertainties derived from the co-operativity observed ...
-
Marcoleta Caldera, Andrés; Berríos Pasten, Camilo; Núñez, Gonzalo; Monasterio Opazo, Octavio; Lagos Mónaco, Rosalba (Frontiers Media, 2016)Due to the developing of multi-resistant and invasive hypervirulent strains, Klebsiella pneumoniae has become one of the most urgent bacterial pathogen threats in the last years. Genomic comparison of a growing number of ...
-
Lagos Mónaco, Rosalba; Wilkens, Marcela; Vergara Montecinos, Cecilia; Cecchi, Ximena; Monasterio Opazo, Octavio (1993)Microcin E492, a polypeptide antibiotic, has been shown to have an Mr, of 6,000 by urea-SDS-polyacrylamide gel electrophoresis of the fluorescently labelled compound. It is known that the bactericidal action of microcin ...
-
Márquez, Ileana F.; Mateos-Gil, Pablo; Shin, Jae Yen; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio; Vélez, Marisela (Elsevier B.V., 2017)© 2017 Elsevier B.V. FtsZ filaments localize at the middle of the bacterial cell and participate in the formation of a contractile ring responsible for cell division. Previous studies demonstrated that the highly conserved ...
-
Díaz Espinoza, Rodrigo; Nova, Esteban; Monasterio Opazo, Octavio (Academic Press Inc., 2017)© 2017 Elsevier Inc. Amyloids are polypeptide aggregates involved in many pathologies including Alzheimer's disease. Amyloid assembly is a complex process affected by different interactions including hydrogen bonding, van ...
-
Araya, G.; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio (Wiley, 2017)
-
Monasterio Opazo, Octavio (Academic Press, 2001)Fast kinetic methods are used to measure reactions that take place in less time than required to mix the reagents manually and to measure the reaction by usual methods, like UV-visible spectrophotometry and fluorescence. ...
-
Andreu, José Manuel; Oliva, María Angela; Monasterio Opazo, Octavio (2002)The stability, refolding, and assembly properties of FtsZ cell division proteins from Methanococcus jannaschii and Escherichia coli have been investigated. Their guanidinium chloride unfolding has been studied by circular ...
-
Acevedo, Mónica L.; Arbildúa, José Jaime; Monasterio Opazo, Octavio; Toledo, Héctor; León, Oscar (2010)X-ray diffraction data on a few retroviral integrases show a flexible loop near the active site. By sequence alignment, the peptide region 207-218 of Mo-MLV IN appears to correspond to this flexible loop. In this study, ...
-
Montecinos Franjola, Felipe Antonio; James, Nicholas G.; Concha Marambio, Luis; Brunet, Juan E.; Lagos Mónaco, Rosalba; Monasterio Opazo, Octavio; Jameson, David M. (Elsevier, 2014)Cell division protein FtsZ cooperatively self-assembles into straight filaments when bound to GTP. A set of conformational changes that are linked to FtsZ GTPase activity are involved in the transition from straight to ...
-
Arranz, Rocío; Mercado, Gabriela; Martín-Benito, Jaime; Giraldo, Rafael; Monasterio Opazo, Octavio; Lagos Mónaco, Rosalba; Valpuesta, José M. (2012)Microcin E492 is a low-molecular weight, channel-forming bacteriotoxin that generates amyloid structures. Using electron microscopy and image processing techniques several structural conformations can be observed. Prior ...
-
Montecinos Franjola, Felipe; Ross, Justin A.; Sánchez, Susana A.; Brunet, Juan E.; Lagos Mónaco, Rosalba; Jameson, David M.; Monasterio Opazo, Octavio (2012-05)FtsZ is a major protein in bacterial cytokinesis that polymerizes into single filaments. A dimer has been proposed to be the nucleating species in FtsZ polymerization. To investigate the influence of the self-assembly of ...
-
Devred, François; Barbier, Pascale; Douillard, Soazig; Monasterio Opazo, Octavio; Andreu, José Manuel; Peyrot, Vincent (2004)Tau is a neuronal microtubule-associated protein that plays a central role in many cellular processes, both physiological and pathological, such as axons stabilization and Alzheimer's disease. Despite extensive studies, ...
-
Monasterio Opazo, Octavio; Acoria García, Marina; Diaz, Marco A.; Lagos Mónaco, Rosalba (1993)The intrinsic fluorescence excitation and emission spectra of chicken brain tubulin showed the characteristic tryptophan fluorescence. The emission spectrum of Tb3+ in the presence of tubulin and GTP excited at 295 nm, ...