Browsing by Author "Magne, Fabien"

Now showing items 1-6 of 6

    • A Real-time PCR assay for detection of low pneumocystis jirovecii levels 
      Ruiz Ruiz, Susana; Ponce Olmos, Carolina Angélica; Pesantes, Nicole; Bustamante, Rebeca; Gatti Orellana, Gianna; San Martin, Viviana; Gutiérrez, Mireya; Bórquez, Pamela; Vargas Munita, Sergio Luis; Magne, Fabien; Calderón, Enrique J.; Pérez Brocal, Vicente; Moya, Andrés (Frontiers Media, 2022)
      Here we report a new real-time PCR assay using SYBR Green which provides higher sensitivity for the specific detection of low levels of Pneumocystis jirovecii. To do so, two primer sets were designed, targeting the family ...
    • Association of gut microbiome and vitamin D deficiency in knee osteoarthritis patients: a pilot study 
      Ramasamy, Boopalan; Magne, Fabien; Tripathy, Sujit Kumar; Venugopal, Giriprasad; Mukherjee, Diptasree; Balamurugan, Ramadass (MDPI, 2021)
      Background: Few preclinical studies have shown that Knee osteoarthritis (KOA) is linked to gut microbiome dysbiosis and chronic inflammation. This pilot study was designed to look at the gut microbiome composition in KOA ...
    • Characterization of human intestinal bifidobacteria using competitive PCR and PCR-TTGE 
      Mangin, Irène; Suau, Antonia; Magne, Fabien; Garrido, Daniel; Gotteland, Martín; Neut, Christel; Pochart, Philippe (BLACKWELL, 2006-01)
      In this study, a competitive PCR was developed to estimate the quantity of bifidobacteria in human faecal samples using two 16S rRNA gene Bifidobacterium genus-specific primers, Bif164f and Bif662r. A PCR-temporal temperature ...
    • Molecularanalysis of intestinalmicrobiota of rainbowtrout (Oncorhynchusmykiss) 
      Navarrete Wallace, Paola; Magne, Fabien; Mardones, Pamela; Riveros, Macarena; Opazo, Rafael; Suau, Antonia; Pochart, Philippe; Romero Ormazábal, Jaime (Blackwell, 2010)
      The aim of this study was to evaluate different molecular tools based on the 16S rRNA gene, internal transcribed spacer, and the rpoB gene to examine the bacterial populations present in juvenile rainbow trout intestines. ...
    • PCR-TTGE Analysis of 16S rRNA from Rainbow Trout (Oncorhynchus mykiss) Gut Microbiota Reveals Host- Specific ... 
      Navarrete Wallace, Paola; Magne, Fabien; Araneda, Cristián; Fuentes, Pamela; Barros, Luis; Espejo Torres, Romilio; Opazo, Rafael; Romero Ormazábal, Jaime (PUBLIC LIBRARY SCIENCE, 2012-02-29)
      This study assessed the relative contributions of host genetics and diet in shaping the gut microbiota of rainbow trout. Full sibling fish from four unrelated families, each consisting of individuals derived from the mating ...
    • Performance of saliva samples for COVID-19 diagnosis by using the AllplexTM 2019-nCoV assay kit 
      Tapia, Cecilia V.; Campos, Marcia; Mora, Ivone; Pozas, Nadia; Morales, Lesly; Guzmán, Camila; Aguilera, Valentina; Ibarra, Paula; Magne, Fabien (Frontiers Media, 2021)
      Background: Although the nasopharyngeal swab (NPS) is the reference sampling method for the detection of SARS-Cov-2, it is not always possible to collect NPS in some patients. Saliva represents an interesting sampling ...