Browsing by Author "Ramírez Sarmiento, César"
Now showing items 1-7 of 7
-
(Springer, 2013)Pyridoxal 50-phosphate is the active form of vitamin B6 and its deficiency is directly related with several human disorders, which make human pyridoxal kinase (hPLK) an important pharmacologic target. In spite of this, ...
-
(Elsevier, 2015)The activity of the ADP-dependent glucokinase from Thermococcus litoralis (TlGK) relies on the highly conserved motifs NXXE (i.e. Asn-Xaa-Xaa-Glu) and HXE (i.e. His-Xaa-Glu). Site-directed mutagenesis of residues Glu279 ...
-
(Elsevier, 2014)In the family of ATP-dependent vitamin kinases, several bifunctional enzymes that phosphorylate hydroxymethyl pyrimidine (HMP) and pyridoxal (PL) have been described besides enzymes specific towards HMP. To determine how ...
-
(Wiley & Sons, 2015)
-
(ACS Publications, 2015)Enzyme-substrate binding is a dynamic process intimately coupled to protein structural changes, which in turn changes the unfolding energy landscape. By the use of single-molecule force spectroscopy (SMFS), we characterize ...
-
(Public Library Science, 2015)RfaH is a virulence factor from Escherichia coli whose C-terminal domain (CTD) undergoes a dramatic alpha-to-beta conformational transformation. The CTD in its alpha-helical fold is stabilized by interactions with the ...
-
Palmitic and stearic acids inhibit chaperone-mediated autophagy (CMA) in POMC-like neurons In vitro (MDPI, 2022)The intake of food with high levels of saturated fatty acids (SatFAs) is associated with the development of obesity and insulin resistance. SatFAs, such as palmitic (PA) and stearic (SA) acids, have been shown to accumulate ...
