Browsing by Author "Rivas Pardo, Jaime"
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Abarca Lagunas, María José; Rivas Pardo, Jaime; Ramírez Sarmiento, César; Giuxé Leguia, Victoria (Elsevier, 2015)The activity of the ADP-dependent glucokinase from Thermococcus litoralis (TlGK) relies on the highly conserved motifs NXXE (i.e. Asn-Xaa-Xaa-Glu) and HXE (i.e. His-Xaa-Glu). Site-directed mutagenesis of residues Glu279 ...
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Rivas Pardo, Jaime; Alegre Cebollada, Jorge; Ramírez Sarmiento, César; Fernández, Julio M.; Guixé Leguía, Victoria Cristina (ACS Publications, 2015)Enzyme-substrate binding is a dynamic process intimately coupled to protein structural changes, which in turn changes the unfolding energy landscape. By the use of single-molecule force spectroscopy (SMFS), we characterize ...