Advanced Search
Now showing items 11-20 of 5996
Notas en torno a la prueba del daño moral: un intento de sistematización
(Universidad Nacional de Educación a Distancia, 2007)
REVISTA DE DERECHO UNED, núm. 2, 2007
NOTAS EN TORNO A LA PRUEBA DEL DAÑO
MORAL: UN INTENTO DE SISTEMATIZACIÓN
HUGO A. CÁRDENAS VILLARREAL
Profesor de Responsabilidad Civil
Universidad Andrés Bello
PAULINA V...
loss, Proof, Evidence * El presente trabajo corresponde a la versión ampliada de la ponencia presenta- da por los autores en las IV Jomadas Nacionales de Derecho Civil (Olmué, Chile, 2006). © UNED. Revista de Derecho UNED, núm. 2, 2007 3 5 5 HUGO A...
loss, Proof, Evidence * El presente trabajo corresponde a la versión ampliada de la ponencia presenta- da por los autores en las IV Jomadas Nacionales de Derecho Civil (Olmué, Chile, 2006). © UNED. Revista de Derecho UNED, núm. 2, 2007 3 5 5 HUGO A...
Definición de dominios de estimación de recursos aplicando técnicas de aprendizaje automático: caso de un depósito epitermal de oro
(Universidad de Chile, 2022)
grados, volviéndose más
subhorizontal en superficie, y un sector NW el cual se caracteriza por presentar una
estructura tabular con tendencia a ser subhorizontal hacia el SE, la cual se une al sector
SE. El tercer dominio identificado corresponde a la...
Caracterización estructural, funcional y farmacológica de un anticuerpo monoclonal humano contra la proteína sST2 en modelos de colitis ulcerosa
(Universidad de Chile, 2023)
descrito largamente sobre el rol del eje ST2L/IL-33 en el desarrollo y grado de actividad de la CU, y se ha creado un anticuerpo monoclonal
humano (mAb) con potenciales acciones moduladoras de sST2 a nivel intestinal, con un alto grado de selectividad y...
La liberación de Calibán-el negro esclavizado y colonizado en Une tempête de Aimé Césaire y The pleasures of exile de Georg Lamming
(Universidad de Chile, 2018)
(1960) y Une tempête (1969), respectivamente. Se trata de un análisis literario y comparativo entre los dos textos con el propósito de investigar la manera en que los dos pensadores antillanos resisten y desmantelan el legado colonial para los...
Comunidad, Memoria y Patrimonio en una aldea rural. La comunidad del Almendral y su perspectiva del patrimonio local
(Universidad de Chile, 2019)
El patrimonio se ha vuelto un ámbito que cobra cada día más adeptos. Se transforma en un comodín que une cultura e identidad, dinamizando símbolos y sensaciones de pertenencia. Seduce como atractivo a cada vez más turistas interesados en...
Studying the role of BiP as a chaperone through single molecule force spectroscopy
(Universidad de Chile, 2016)
P se une a las proteínas y estabiliza
su estado desplegado, luego se desune para darles la oportunidad de plegarse
espontáneamente. Si las proteínas son incapaces de hacerlo BiP se une una vez más, evitando
así el plegamiento erróneo y la agregación.
El...
Quality control and proteostasis are crucial processes in the cell to maintain homeostasis, and chaperones have a key role in this. BiP (Immunoglobulin Binding Protein) is an ER-located (Endoplasmic Reticulum) member of the family of HSP70 molecular chaperones that participates is various processes, such as protein folding and activation of the unfolded protein response. BiP binds to proteins and stabilizes their unfolded state, then unbinds to give them a chance to spontaneously fold. If proteins are unable to do so BiP binds once more, avoiding misfolding and aggregation. The aim of this project was to study the function of BiP as a chaperone. It was unknown how BiP exactly affected the protein folding pathway and it had not been directly demonstrated to which folding state of the substrate protein it binds. In addition, to our knowledge all studies regarding BiP binding and affinity have been done using peptides and not full proteins. For this, optical tweezers for single molecule force spectroscopy experiments were employed to investigate how BiP affects the folding mechanism of a protein and how this effect depends on nucleotides. Using the protein MJ0366 as BiP’s substrate, which most likely has just one binding site, pulling and relaxing cycles at constant velocity to unfold and refold the protein substrate were performed. In the absence of BiP, MJ0366 unfolded and refolded in every pulling cycle. However, when BiP was added the frequency of folding events of MJ0366 significantly decreased. The loss in folding always occurred after a successful unfolding event. This process was dependent on ATP and ADP concentrations, since when either ATP was decreased or ADP was added, the duration of periods without folding events increased. The observed effect was due to the binding of BiP to MJ0366 and not nonspecific interactions (BSA or lysozyme, used as controls). These results show that the affinity for the substrate protein increased in these conditions. Therefore, we conclude that BiP binds to the unfolded state of MJ0366 and prevents its refolding and that this effect depends on the type and concentration of nucleotides. In summary, BiP chaperone binding/release has a very clear effect in protein folding, after mechanical unfolding of the protein MJ0366 BiP is able to find its binding site, or sites (the number of binding sites has not been experimentally demonstrated in this work), and bind in a reversible manner, avoiding the formation of tertiary contacts. Future studies will be performed with different concentrations of BiP to confirm that there is only one binding site for BiP in MJ0366 and so, empirically validate our model and calculated affinity constants...
Quality control and proteostasis are crucial processes in the cell to maintain homeostasis, and chaperones have a key role in this. BiP (Immunoglobulin Binding Protein) is an ER-located (Endoplasmic Reticulum) member of the family of HSP70 molecular chaperones that participates is various processes, such as protein folding and activation of the unfolded protein response. BiP binds to proteins and stabilizes their unfolded state, then unbinds to give them a chance to spontaneously fold. If proteins are unable to do so BiP binds once more, avoiding misfolding and aggregation. The aim of this project was to study the function of BiP as a chaperone. It was unknown how BiP exactly affected the protein folding pathway and it had not been directly demonstrated to which folding state of the substrate protein it binds. In addition, to our knowledge all studies regarding BiP binding and affinity have been done using peptides and not full proteins. For this, optical tweezers for single molecule force spectroscopy experiments were employed to investigate how BiP affects the folding mechanism of a protein and how this effect depends on nucleotides. Using the protein MJ0366 as BiP’s substrate, which most likely has just one binding site, pulling and relaxing cycles at constant velocity to unfold and refold the protein substrate were performed. In the absence of BiP, MJ0366 unfolded and refolded in every pulling cycle. However, when BiP was added the frequency of folding events of MJ0366 significantly decreased. The loss in folding always occurred after a successful unfolding event. This process was dependent on ATP and ADP concentrations, since when either ATP was decreased or ADP was added, the duration of periods without folding events increased. The observed effect was due to the binding of BiP to MJ0366 and not nonspecific interactions (BSA or lysozyme, used as controls). These results show that the affinity for the substrate protein increased in these conditions. Therefore, we conclude that BiP binds to the unfolded state of MJ0366 and prevents its refolding and that this effect depends on the type and concentration of nucleotides. In summary, BiP chaperone binding/release has a very clear effect in protein folding, after mechanical unfolding of the protein MJ0366 BiP is able to find its binding site, or sites (the number of binding sites has not been experimentally demonstrated in this work), and bind in a reversible manner, avoiding the formation of tertiary contacts. Future studies will be performed with different concentrations of BiP to confirm that there is only one binding site for BiP in MJ0366 and so, empirically validate our model and calculated affinity constants...
Fundamento- Sin fundamento, el juego del vacío
(Universidad de Chile, 2004)
él. Ya sabemos que el puente acerca a una orilla de la otra, pero la acerca porque está separada y aquello que las separa, mienta a la vez de aquello que las une. Pensemos en un río y construyamos en nuestra mente un puente. Ahora, atrevámonos a...
Los girasoles ciegos de Alberto Méndez: diálogo con la memoria colectiva y textos referenciales como recurso de verosimilitud
(Universidad de Chile, 2012)
girasoles ciegos es un libro que une sutilmente cuatro narraciones sobre la Guerra Civil Española y sus consecuencias políticas y sociales, encarnadas en personajes contradictorios que caminan al borde de la locura, encontrando en la derrota un factor común...
Deleuze : filosofía de la creación
(Universidad de Chile, 2013)
En la presente investigación nos proponemos mostrar al pensamiento de Gilles Deleuze como una filosofía de la creación. Ésta se plantea como desafío el desmontaje del modelo ego-logo/céntrico de pensamiento, asumiendo el cuestionamiento acerca de...
Movilidad Intergeneracional de Ingresos y Asociación de Parejas: Evidencia para Chile
(Universidad de Chile, 2009)
En el presente trabajo desarrollo un modelo que une las literaturas de movilidad intergeneracional y assortative mating. Dicho modelo me permite analizar la elasticidad de ingresos padre-hijo desde una perspectiva un poco más amplia y más completa...