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Campanadas del mar. Lecturas filosóficas de la poesía de Pablo Neruda
(Universidad de Chile, Facultad de Filosofía y Humanidades, 1996)
losófico.
Esta tarea la cumple Eduardo Carrasco en forma tan bella como rigurosa. Su libr11
es la campanada que, desde la filosofía, abre el camino para una hemu~n~uti~.:a uc la
poesía de Neruda y, en general, para una reflexión fil osófica en torno u la...
CAMPANADAS DEL MAR. LECTURAS FILOSÓFICAS DE LA POESÍA DE PABLO NERUDA. por Edmml" CIIITll 1CO San11apo d<! Chllt- Gntpu Edltonal 7cr" S. A 1\1'15 r;,'ii Campanadas del Mar se declara en el subtítulo mismo y en la...
CAMPANADAS DEL MAR. LECTURAS FILOSÓFICAS DE LA POESÍA DE PABLO NERUDA. por Edmml" CIIITll 1CO San11apo d<! Chllt- Gntpu Edltonal 7cr" S. A 1\1'15 r;,'ii Campanadas del Mar se declara en el subtítulo mismo y en la...
Influencia de la geología y geotecnia en parámetros de chancabilidad en yacimiento El Teniente, VI Región Chile
(Universidad de Chile, 2020)
El proceso de chancado representa el 60% del costo energético de una planta de procesamiento, siendo necesaria su optimización. Para ello se ocupan parámetros que miden la chancabilidad de un tipo de roca, como el Chancado LEIT (Cwi) o el Índice de...
Impacto económico y operacional de sistemas de almacenamiento de energía frente a un escenario de alta penetración eólica en el SIC-Chile, utilizando un modelo de coordinación hidrotérmico
(Universidad de Chile, 2014)
objetivo general de este trabajo es modificar el modelo hidrotérmico de predespacho MIP-UC del Centro de Energía de la FCFM de la universidad de Chile, para poder estudiar los efectos del uso de sistemas de almacenamiento en el sistema interconectado...
Leaf litter is essential for seed survival of the endemic endangered tree pouteria splendens (Sapotaceae) from central Chile
(Copernicus Gesellschaft MBH, 2018)
d
s
ee
d
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Under canopy
Figure 1. Dispersed seeds (mean±SD) collected in traps, under
and outside the canopy of P. splendens in Los Molles.
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0 5 10 15 20
Se
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s
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Plains: OC
Plains: UC
Plains: UC-L
Rocky...
mounds: OC Rocky mounds: UC Rocky mounds: UC-L Figure 2. Seed survival curves (mean±SD) under the canopy of P. splendens, with and without leaf litter, and outside the canopy, both in plains and rocky mounds. UC, under canopy without leaf litter; UC...
mounds: OC Rocky mounds: UC Rocky mounds: UC-L Figure 2. Seed survival curves (mean±SD) under the canopy of P. splendens, with and without leaf litter, and outside the canopy, both in plains and rocky mounds. UC, under canopy without leaf litter; UC...
Precios de productos almacenables: implicaciones del modelo de inventarios
(Universidad de Chile. Facultad de Economía y Negocios, 2004-06)
factor de descuento
0 < δ <1. En una especificación particular del modelo, la función de utilidad de
consumidor representativo, U: R+→R+, es continua, continuamente derivable, es-
trictamente creciente y estrictamente cóncava, con U(0) = 0, y limc→∞ U(c...
) = B<+∞. La función (inversa) de demanda por consumo es f = U’. Notar que en este caso U(c) = 0 c∫ f(ξ)dξ, y que el ingreso total por ventas, cf(c), está uniformemente acotado por la constante B. El mercado competitivo entrega la misma solución que la...
) = B<+∞. La función (inversa) de demanda por consumo es f = U’. Notar que en este caso U(c) = 0 c∫ f(ξ)dξ, y que el ingreso total por ventas, cf(c), está uniformemente acotado por la constante B. El mercado competitivo entrega la misma solución que la...
Efectos de requerimientos para centrales fotovoltaicas en la estabilidad de tensión del Sistema Interconectado del Norte Grande
(Universidad de Chile, 2013)
El uso excesivo de combustibles fósiles y la crisis ambiental a nivel mundial han activado una señal de alerta, por lo que los países han comenzado a considerar medidas de mitigación y Chile no es la excepción. Por otro lado, a nivel nacional...
Efecto de la infección del virus de la necrosis pancreática infecciosa (IPNV) en la traducción de los mRNAs de su célula hospedero CHSE/F
(2018)
En nuestro país, la necrosis pancreática infecciosa (IPN) es una enfermedad endémica, prevalente y de importancia económica, ya que está ampliamente distribuida en las salmoniculturas chilenas. El virus de la necrosis pancreática infecciosa (IPNV...
In our country, infectious pancreatic necrosis (IPN) is an endemic, prevalent and economically important disease that is widely distributed in Chilean salmon farms. The infectious pancreatic necrosis virus (IPNV) belongs to the family Birnaviridae, genus Aquabirnavirus. It is characterized by having no lipid envelope and a diameter close to 60 nm. Its genome is composed of two segments of double-stranded RNA, segment A and B. The 5 'ends of each segment is covalently linked to the viral protein Vpg of 94 kDa. The messenger RNA (mRNA) does not have a polyadenine tail or CAP. It has been shown that viral infection inhibits the synthesis of cellular proteins at the same time that viral polypeptides begin to be detected, therefore it is expected that the mechanism of viral translation initiation will be different from that of the host cell. Although there have been studies that seek to understand this phenomenon, it has not been described what viral protein could be responsible for it. Based on this background, this title seminar aimed to evaluate the effect of viral infection and the particular contribution of viral proteins VP1, pVP2, VP2 and VP4 in the translation of reporter mRNAs whose translation was dependent on CAP or the internal ribosome entry site (IRES). To this end, the expression of bicistronic reporters (dual luc or dL) containing the IRES of the Cricket palsy virus (CrPV) and the hepatitis C virus (HCV) was evaluated. The cell line derived from Lepomis macrochirus embryonic cells, CHSE / F, which is susceptible to IPNV infection was used. The cells were infected or transfected with plasmids coding for the viral proteins. For the transfection experiments, plasmids coding for VP1 and VP4 that were used were those already counted in the laboratory and plasmids coding for the structural protein VP2 and its pVP2 precursor was generated by bacterial cloning. These bacterial vectors were evaluated by PCR and immunofluorescence. In the CHSE / F cell infection experiment, a decrease in CAP-dependent translation and xix an increase in IRES-dependent translation was observed. On the other hand, with the transfection experiment it was recorded that VP1 and VP4 inhibit the CAP-dependent translation and stimulate the IRES-dependent translation. In the case of the tests carried out with VP2 and pVP2, in the presence of the precursor structural protein, the CAP dependent translation decreased by half, whereas when transfecting VP2 there were no significant changes. The results regarding IRES dependent translation, in the presence of structural proteins are unclear. With pVP2 no significant changes were reported, and with VP2 the translation increased for 1 of the reporters (dlCrPV), while it decreased for the other (dlHCV). These results show that viral proteins affect the translation of mRNAs in different ways, suggesting that they are responsible for the inhibition of CAP dependent translation and the stimulation of IRES-dependent translation....
In our country, infectious pancreatic necrosis (IPN) is an endemic, prevalent and economically important disease that is widely distributed in Chilean salmon farms. The infectious pancreatic necrosis virus (IPNV) belongs to the family Birnaviridae, genus Aquabirnavirus. It is characterized by having no lipid envelope and a diameter close to 60 nm. Its genome is composed of two segments of double-stranded RNA, segment A and B. The 5 'ends of each segment is covalently linked to the viral protein Vpg of 94 kDa. The messenger RNA (mRNA) does not have a polyadenine tail or CAP. It has been shown that viral infection inhibits the synthesis of cellular proteins at the same time that viral polypeptides begin to be detected, therefore it is expected that the mechanism of viral translation initiation will be different from that of the host cell. Although there have been studies that seek to understand this phenomenon, it has not been described what viral protein could be responsible for it. Based on this background, this title seminar aimed to evaluate the effect of viral infection and the particular contribution of viral proteins VP1, pVP2, VP2 and VP4 in the translation of reporter mRNAs whose translation was dependent on CAP or the internal ribosome entry site (IRES). To this end, the expression of bicistronic reporters (dual luc or dL) containing the IRES of the Cricket palsy virus (CrPV) and the hepatitis C virus (HCV) was evaluated. The cell line derived from Lepomis macrochirus embryonic cells, CHSE / F, which is susceptible to IPNV infection was used. The cells were infected or transfected with plasmids coding for the viral proteins. For the transfection experiments, plasmids coding for VP1 and VP4 that were used were those already counted in the laboratory and plasmids coding for the structural protein VP2 and its pVP2 precursor was generated by bacterial cloning. These bacterial vectors were evaluated by PCR and immunofluorescence. In the CHSE / F cell infection experiment, a decrease in CAP-dependent translation and xix an increase in IRES-dependent translation was observed. On the other hand, with the transfection experiment it was recorded that VP1 and VP4 inhibit the CAP-dependent translation and stimulate the IRES-dependent translation. In the case of the tests carried out with VP2 and pVP2, in the presence of the precursor structural protein, the CAP dependent translation decreased by half, whereas when transfecting VP2 there were no significant changes. The results regarding IRES dependent translation, in the presence of structural proteins are unclear. With pVP2 no significant changes were reported, and with VP2 the translation increased for 1 of the reporters (dlCrPV), while it decreased for the other (dlHCV). These results show that viral proteins affect the translation of mRNAs in different ways, suggesting that they are responsible for the inhibition of CAP dependent translation and the stimulation of IRES-dependent translation....
Introducción. Metodología de Diseño Arquitectónico Edwin Haramoto: Adopciones y adaptaciones
(Adrede Editora, 2018)
En el marco de la celebración de los 30 años del Instituto de la Vivienda INVI, el libro “Metodología de diseño Edwin Haramoto. Adopciones y Adaptaciones” intenta homenajear al Arquitecto Edwin Haramoto Nishikimoto (Q.E.P.D.) quien fuera uno de los...
Factores explicativos de la distribución espacial de la temperatura del aire de verano en Santiago de Chile
(Pontificia Universidad Católica de Chile. Instituto de Geografía, 2016)
Santiago de Chile presenta importantes diferencias en la distribución de las temperaturas del aire en el interior de la ciudad debido a que un conjunto de factores explicativos, como el incremento de las tasas de impermeabilización y la reducción de...
The city of Santiago (Chile) shows significant differences in the distribution of air temperatures within the city due to a set of explanatory factors, such as increased proportions of impermeable surfaces and a reduction in vegetation. This has in turn generated heat islands, which are accentuated and generalized in the summer nights, creating a complex and heterogeneous mosaic of urban landscapes that affect the environment and quality of life of its inhabitants. Based on networks of urban-climate information that link data from fixed stations, mobile transects and thermal satellite imagery, we estimate significant spatial relationships that have allowed us to construct maps that help to explain the temporal and spatial variability of atmospheric temperatures, and thus to suggest measures for mitigating and adapting to the phenomena at both the macro- and meso-scale....
The city of Santiago (Chile) shows significant differences in the distribution of air temperatures within the city due to a set of explanatory factors, such as increased proportions of impermeable surfaces and a reduction in vegetation. This has in turn generated heat islands, which are accentuated and generalized in the summer nights, creating a complex and heterogeneous mosaic of urban landscapes that affect the environment and quality of life of its inhabitants. Based on networks of urban-climate information that link data from fixed stations, mobile transects and thermal satellite imagery, we estimate significant spatial relationships that have allowed us to construct maps that help to explain the temporal and spatial variability of atmospheric temperatures, and thus to suggest measures for mitigating and adapting to the phenomena at both the macro- and meso-scale....
Santiago de Chile en disputa: de la avalancha neoliberal a las alternativas de resistencia y auto-gestión
(2017-05)
Durante la última década, la sociedad chilena ha estado viviendo en un fuerte estado de crisis. Una serie de escándalos de corrupción política, colusión económica y prácticas anti-democráticas, sumada a la incesante reproducción de desigualdades y...