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Las moléculas HLA-DR que expresan alelos del epítopo compartido, favorecen la presentación de péptidos de alta afinidad derivados de proteínas en tejido y fluido sinovial de pacientes con artritis reumatoide
(Universidad de Chile, 2016)
Estudiamos el peptidoma asociado a la molécula HLA-DR, tanto en tejido sinovial de pacientes con artritis reumatoide (AR) como en células dendríticas (CD) de donantes positivos para alelos del epítopo compartido (EC), pulsadas con fluido sinovial de...
This study involves the peptidome associated to HLA-DR molecule, in synovial tissue from Rheumatoid Arthritis (RA) patients and dendritic cells (DC) from healthy donors positive for shared epitope (SE) alleles pulsed with synovial fluid from RA patients. These alleles have been extensively studied, finding an association with RA. We using _-mass spectrometric-_ peptide repertoire describe some alleles of EC, obtained from tissue and CDs from healthy donors pulsed with FS. Based on the type of binding of these peptide sequences and their origin, we see own of these alleles, they exhibited one more akin to theoretical peptide sequences binding characteristics. This will enable us in the near future the development of a search for new potential autoantigens for this disease. Methodology: 3 RA patients selected for joint replacement surgery were recruited, genotyped for HLA-DR gene, and their synovial fluid and tissue were obtained. Also, healthy donors positive for SE alleles were recruited. From the 100 selected subjects, blood samples were obtained to generate DC, which were pulsed with synovial fluid from RA patients or left unpulsed. HLA-DR/peptides complexes were isolated from tissue and cells, later the peptides which were sequenced by mass spectrometry were eluted and analyzed by bioinformatics tools. Results: 1176 peptides from 227 parental proteins were obtained. Most peptides were presented in nested set and derived from proteins processed by the endocytic pathway. In the case of synovial tissue, most peptides derive from the extracellular medium, whereas in DC, 70% were derived from the plasma membrane. Most peptides presented by SE alleles DRB1*01:01 and DRB1*04:01 in synovial tissue were defined as of high and intermediate affinity for their respective HLA-DR allele. In DC, the peptides presented by the allele DRB1*01:01 had a high theoretical affinity for this allele. No citrullinated peptides were detected neither in synovial tissue nor in pulsed and non-pulsed DC. Conclusion: The peptidome associated to HLA - DR molecule of alleles SE presents peptide sequences with high theoretical affinity. The sequences presented in tissues derived from the extracellular medium and interacting with inflammatory proteins. The sequences presented in healthy donor CDs mostly come from plasma membrane proteins and are related to processing and antigen presentation....
This study involves the peptidome associated to HLA-DR molecule, in synovial tissue from Rheumatoid Arthritis (RA) patients and dendritic cells (DC) from healthy donors positive for shared epitope (SE) alleles pulsed with synovial fluid from RA patients. These alleles have been extensively studied, finding an association with RA. We using _-mass spectrometric-_ peptide repertoire describe some alleles of EC, obtained from tissue and CDs from healthy donors pulsed with FS. Based on the type of binding of these peptide sequences and their origin, we see own of these alleles, they exhibited one more akin to theoretical peptide sequences binding characteristics. This will enable us in the near future the development of a search for new potential autoantigens for this disease. Methodology: 3 RA patients selected for joint replacement surgery were recruited, genotyped for HLA-DR gene, and their synovial fluid and tissue were obtained. Also, healthy donors positive for SE alleles were recruited. From the 100 selected subjects, blood samples were obtained to generate DC, which were pulsed with synovial fluid from RA patients or left unpulsed. HLA-DR/peptides complexes were isolated from tissue and cells, later the peptides which were sequenced by mass spectrometry were eluted and analyzed by bioinformatics tools. Results: 1176 peptides from 227 parental proteins were obtained. Most peptides were presented in nested set and derived from proteins processed by the endocytic pathway. In the case of synovial tissue, most peptides derive from the extracellular medium, whereas in DC, 70% were derived from the plasma membrane. Most peptides presented by SE alleles DRB1*01:01 and DRB1*04:01 in synovial tissue were defined as of high and intermediate affinity for their respective HLA-DR allele. In DC, the peptides presented by the allele DRB1*01:01 had a high theoretical affinity for this allele. No citrullinated peptides were detected neither in synovial tissue nor in pulsed and non-pulsed DC. Conclusion: The peptidome associated to HLA - DR molecule of alleles SE presents peptide sequences with high theoretical affinity. The sequences presented in tissues derived from the extracellular medium and interacting with inflammatory proteins. The sequences presented in healthy donor CDs mostly come from plasma membrane proteins and are related to processing and antigen presentation....
Identificación de especies del género Mytilus utilizando marcadores moleculares mitocondriales y nucleares
(Universidad de Chile, 2013)
Los mitílidos son una de las especies de bivalvos más cultivadas y comercializadas. Existen variedad de mercados que exigen la veracidad de la información contenida en el etiquetado de estos productos, es por ello que independientemente del estatus...
The mussels are one of the most cultivated and world wide commercialized species of bivalves. There are variety of countries of destination that require the accuracy of the information contained in the labeling of these products, which is why regardless of the taxonomic status that the mussel species have in Chile, it is important for the industry to establish a truthful labeling and that represent accurately the origin and identification of the species of the product to be consumed. An evaluation of the most internationally used molecular markers for species identification in mussels samples from Chile, Canada and Galicia was performed to determine the equivalence and quality of identifying these markers comparing them with the gene of the polyphenolic adhesive protein (Me 15-16 AciI). Ti was evaluated as well the ability to identify species with markers Me AciI 15-16 and COI XbaI in processed products . The most informative panel of markers evaluated were Me 15-16 AciI and mac- 1 combined. The nuclear marker mac-1 is equivalent to Me 15-16 AciI in identified M. galloprovincialis. Meanwhile mitochondrial marker COI XbaI and mtDNA 16s rRNA are fully equivalent to Me 15 -16 AciI to identified M. chilensis from other species. Me 15 16 AciI and COI XbaI can be used processed raw, smoked in oil and frozen. The amplification of large fragments from markers like mac -1 and mtDNA 16s rRNA in processed products is not applicable. The use of Me 15-16 AciI in processed products is recommended...
The mussels are one of the most cultivated and world wide commercialized species of bivalves. There are variety of countries of destination that require the accuracy of the information contained in the labeling of these products, which is why regardless of the taxonomic status that the mussel species have in Chile, it is important for the industry to establish a truthful labeling and that represent accurately the origin and identification of the species of the product to be consumed. An evaluation of the most internationally used molecular markers for species identification in mussels samples from Chile, Canada and Galicia was performed to determine the equivalence and quality of identifying these markers comparing them with the gene of the polyphenolic adhesive protein (Me 15-16 AciI). Ti was evaluated as well the ability to identify species with markers Me AciI 15-16 and COI XbaI in processed products . The most informative panel of markers evaluated were Me 15-16 AciI and mac- 1 combined. The nuclear marker mac-1 is equivalent to Me 15-16 AciI in identified M. galloprovincialis. Meanwhile mitochondrial marker COI XbaI and mtDNA 16s rRNA are fully equivalent to Me 15 -16 AciI to identified M. chilensis from other species. Me 15 16 AciI and COI XbaI can be used processed raw, smoked in oil and frozen. The amplification of large fragments from markers like mac -1 and mtDNA 16s rRNA in processed products is not applicable. The use of Me 15-16 AciI in processed products is recommended...
Bioconversión de carbohidratos de macroalgas pardas en ácido hialurónicoutilizando una cepa de Escherichia Coli recombinante
(Universidad de Chile, 2017)
En la actualidad, se realizan grandes esfuerzos en aplicar nuevos métodos de producción de compuestos novedosos de alto valor agregado. Un ejemplo de esto es el ácido hialurónico, que tiene importantes y nuevos usos, especialmente en el área de la...
Evaluación del uso de NaSH en el circuito de flotación selectiva de molibdeno de Los Pelambres
(Universidad de Chile, 2015)
el impacto sobre la flotación al emplear aguas con reactivos residuales correspondientes a otros procesos de la planta. Los resultados ante este nuevo escenario planteado, indican que un cambio parcial y/o total de las aguas de flotación no afectan la...
Aplicación móvil para registro, control y gestión de información de mantenimiento
(Universidad de Chile, 2016-07-22)
,
confiabilidad/monitoreo, seguridad / Medio Ambiente y Recursos Humanos.
Socio 2: Mauricio Palacios Sessarego, Ingeniero Civil Mecánico de la Universidad de Santiago
de Chile, con más de 7 años de experiencia en operación de plantas y áreas de Mantenimiento...
gobierno se relaciona con las propuestas de reformas estructurales en las áreas laboral, educacional y a la constitución política del Estado. Existe un crítico diagnóstico sobre cómo percibe la gente las reformas estructurales que impulsa la Presidenta M...
gobierno se relaciona con las propuestas de reformas estructurales en las áreas laboral, educacional y a la constitución política del Estado. Existe un crítico diagnóstico sobre cómo percibe la gente las reformas estructurales que impulsa la Presidenta M...
Caracterización y agregación de modelos geológicos para una exploración minera temprana
(Universidad de Chile, 2017)
según su correlación con los modelos de yacimientos. Al ser unas más relevantes que otras, se establecieron órdenes jerárquicos sobre ellas. Así, aquellas más relevantes segmentarán los modelos con mayor magnitud. La segmentación permitirá delinear los...
Optimización de la tabla quirúrgica de los pabellones de cirugías para clínica privada en Chile
(Universidad de Chile, 2016)
dinamismo importante, con
3
constantes flujos de inversiones que generan un aumento sostenido de la oferta en
todo tipo de establecimientos
En la Tabla 1 e Ilustración 4, se instruirá sobre la oferta disponible de
prestadores de salud privada, o sea...
cantidad de prestadores privados, se utilizó la información entregada por cada Clínica sobre prestaciones médicas. Cuando se considera las atenciones hospitalarias atendidas, la Clínica Santa María es la segunda que realiza mayor cantidad de cirugías...
cantidad de prestadores privados, se utilizó la información entregada por cada Clínica sobre prestaciones médicas. Cuando se considera las atenciones hospitalarias atendidas, la Clínica Santa María es la segunda que realiza mayor cantidad de cirugías...
Trachytic lavas of the Quetrupillán Volcanic Complex, Chile (39°30'S): Examples of rejuvenation of a crystalline mush reservoir
(Universidad de Chile, 2017)
isotópicos de 143Nd/144Nd, lo que ha sido atribuido a un cambio W-E gradual en los fluidos derivados del slab o a un aumento de la contaminación del magma por el manto litosférico. El Centro eruptivo menor de Huililco es el centro más cercano al volcán...
Naturaleza, distribución espacial e implicaciones Petrogenéticas de los enclaves máficos microgranulares del complejo plutónico Illapel, cordillera de la costa, Chile central
(Universidad de Chile, 2011)
magmas máficos en la génesis y evolución de los granitoides calco–alcalinos. El objetivo de esta investigación es elaborar un modelo petrogenético para la formación y evolución de los EMM y, de esta forma, insertarlo dentro del contexto general de...
Las aguas termales de la isla de Isquia (Sur Italia): Origen y procesos hidrogeoquímicos
(Universidad de Chile, 2016)
Isquia constituye un vigoroso sistema hidrotermal del cual emergen una serie de manifestaciones termales que han llevado y sustentado una intensa actividad turística; principal sustento económico de la misma. En la actualidad se encuentra en...