Advanced Search
Now showing items 61-70 of 324
Purificación, caracterización bioquímica y evaluación de la citotoxicidad del peptido antimicrobiano gicina A
(Universidad de Chile, 2011)
Guías de práctica clínica basadas en evidencia
(Sociedad Odontológica de Chile, 2009-11)
Enseñanza virtual de la bioética. Desafíos
(PAN AMER HEALTH ORGANIZATION, 2008)
Efecto del ácido poliacrílico y de quitosano sobre la electrocristalización de carbonato cálcico
(Universidad de Chile, 2015)
Dimensiones culturales de la donación de órganos post morten. En la respuesta afirmativa de los deudos
(Universidad de Chile, 2018)
Aislamiento de exosomas con nanopartículas de oro funcionalizadas obtenidos desde la línea celular B16F10
(Universidad de Chile, 2017)
de acción. Por otro lado, las nanopartículas de oro (AuNPs) son candidatos interesantes para su aplicación en biomedicina debido a sus múltiples propiedades fisicoquímicas que le permiten ser empleadas para terapia y diagnóstico. En estudios...
Exosomes are nanometric extracellular vesicles involved in cell communication. The development of “nanovehicles”, such as the exosomes, is an innovative strategy to optimize the drug delivery to specific action sites. On another hand, gold nanoparticles (AuNPs) are interesting candidate for biomedical applications due to its physicochemical properties that allow them to be used for therapy and diagnosis. Previous studies have shown that isolated exosomes from B16F10 cells are accumulated preferentially in the lungs. Consequently, this nanovesicles could be used for the selective delivery of bioactive compounds such as the gold nanoparticles (AuNPs). Therefore, the hypothesis of this work suggests that it is possible to isolate exosomes containing functionalized gold nanoparticles from the supernatant of the B16F10 cell line. From the above, the goal of this project was developed a strategy for the incorporation of gold nanoparticles (AuNPs) into exosomes that permitting improve the drug delivery of them. For this, we developed functionalized AuNPs with folic acid or R7CLPFFD peptide and later those AuNPs were incubated with B16F10 cell culture. Then, we isolated the nanovesicles by two methods: ultracentrifugation and using the Exospin® kit. Both samples were characterized by dynamic light scattering, zeta potential, transmission electronic microscopy and western blotting. Later, we evaluated the presence of gold in the isolated exosomes samples by UV-visible spectrophotometry and neutronic activation. As result of the proposed strategy, we isolated exosomes containing gold nanoparticles from the B16F10 culture supernatants. Finally, we expect that this nanosystem could be used for the selective delivery of active compounds to the lungs...
Exosomes are nanometric extracellular vesicles involved in cell communication. The development of “nanovehicles”, such as the exosomes, is an innovative strategy to optimize the drug delivery to specific action sites. On another hand, gold nanoparticles (AuNPs) are interesting candidate for biomedical applications due to its physicochemical properties that allow them to be used for therapy and diagnosis. Previous studies have shown that isolated exosomes from B16F10 cells are accumulated preferentially in the lungs. Consequently, this nanovesicles could be used for the selective delivery of bioactive compounds such as the gold nanoparticles (AuNPs). Therefore, the hypothesis of this work suggests that it is possible to isolate exosomes containing functionalized gold nanoparticles from the supernatant of the B16F10 cell line. From the above, the goal of this project was developed a strategy for the incorporation of gold nanoparticles (AuNPs) into exosomes that permitting improve the drug delivery of them. For this, we developed functionalized AuNPs with folic acid or R7CLPFFD peptide and later those AuNPs were incubated with B16F10 cell culture. Then, we isolated the nanovesicles by two methods: ultracentrifugation and using the Exospin® kit. Both samples were characterized by dynamic light scattering, zeta potential, transmission electronic microscopy and western blotting. Later, we evaluated the presence of gold in the isolated exosomes samples by UV-visible spectrophotometry and neutronic activation. As result of the proposed strategy, we isolated exosomes containing gold nanoparticles from the B16F10 culture supernatants. Finally, we expect that this nanosystem could be used for the selective delivery of active compounds to the lungs...
Una comunidad, una misión, un país. Universidad de Chile 2014-2018
(Universidad de Chile, 2018)
Bioética en Latinoamérica : articulaciones entre bioética, biopolítica y colonialidad
(Universidad de Chile, 2017)