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Análisis experimental del comportamiento tribocorrosivo de recubrimientos fabricados por MXenos aplicados sobre acero inoxidable
(Universidad de Chile, 2024)
estrategias efectivas de prevención y cuidado para asegurar la integridad y longevidad de los componentes de acero inoxidable en uso.
La comprensión del comportamiento del acero inoxidable en entornos corrosivos es esencial para desarrollar alternativas...
Riesgo de osteonecrosis mandibular relacionada a medicamentos en pacientes con osteoporosis tratados con Denosumab: resumen estructurado de evidencia
(Universidad de Chile, 2024)
más utilizada para la prevención y tratamiento de la osteoporosis es la
terapia con denosumab y bifosfonatos nitrogenados. Se han relacionado con la
osteonecrosis mandibular relacionada a medicamentos, la cual es una complicación
infrecuente y...
Efecto de la digestión in vitro sobre el potencial antioxidante y antiinflamatorio de un extracto rico en florotaninos obtenido de algas pardas (Durvillaea Incurvata) de las costas de Concepción
(2023)
-α sugiere un potencial efecto antiinflamatorio del extracto digerido de Durvillaea incurvata, con aplicaciones prometedoras en la prevención o manejo de trastornos digestivos asociados al envejecimiento....
Introduction: Population aging has a significant impact on the digestive system, particularly in terms of intestinal digestion and absorption. Phlorotannins, a type of polyphenol exclusive to brown seaweeds, possess antioxidant and anti-inflammatory properties, making them potential agents to mitigate gastrointestinal alterations associated with aging. Objective: To evaluate the effect of in vitro digestion under simulated elderly conditions on the stability, bioaccessibility, and functionality of Phlorotannins extracted from Durvillaea incurvata by ultrasound-assisted extraction (USAE) and their antioxidant and anti-inflammatory impact in an inflamed intestinal model. Materials and Methods: The extract was characterized for proximal composition, total polyphenol content (TPC), total phlorotannins (PhT), and antioxidant capacity (AOC). Non-cytotoxic concentrations were determined by MTT assay in Caco-2 and HT-29 cells. Digestion was performed according to the INFOGEST 2.0 protocol, adapted for elderly conditions (EC), including dialysis, to estimate absorbable fractions and calculate recovery (RR) and bioaccessibility index (BI). ELISA was used to evaluate IL-1β and TNF-α production in a Transwell system with inflamed epithelium. Results: The crude extract demonstrated 24.26 ± 0.84 mg EF/g TPC, 0.91 ± 0.05 mg EF/g PhT, and 17.67 ± 0.25 mg TE/g AOC, along with 45.5 g/100 of insoluble fiber. Safe concentrations were ≤1 g/100 mL for Caco-2 and ≤1.2 g/100 mL for HT-29. Following digestion, TPC and AOC increased significantly (p<0.05) by 190% and 71% respectively, indicating greater bioaccessibility in the elderly model vs adult model. PhT also increased (p<0.05) but demonstrated low bioaccessibility (~2%). IL-1β showed a temporary rise at 6 h, followed by a decline at 24 h, particularly in the crude extract (-45.2%) and elderly digestion (-24.7%). TNF-α appeared to decrease, achieving the most significant reduction at 12 h in the extract (-23.4%), though without significant differences. Conclusion: Simulated digestion improved the release and bioaccessibility of bioactive compounds, especially in the elderly model. The temporal modulation of IL-1β and TNF-α suggests a potential anti-inflammatory effect of the digested Durvillaea incurvata...
Introduction: Population aging has a significant impact on the digestive system, particularly in terms of intestinal digestion and absorption. Phlorotannins, a type of polyphenol exclusive to brown seaweeds, possess antioxidant and anti-inflammatory properties, making them potential agents to mitigate gastrointestinal alterations associated with aging. Objective: To evaluate the effect of in vitro digestion under simulated elderly conditions on the stability, bioaccessibility, and functionality of Phlorotannins extracted from Durvillaea incurvata by ultrasound-assisted extraction (USAE) and their antioxidant and anti-inflammatory impact in an inflamed intestinal model. Materials and Methods: The extract was characterized for proximal composition, total polyphenol content (TPC), total phlorotannins (PhT), and antioxidant capacity (AOC). Non-cytotoxic concentrations were determined by MTT assay in Caco-2 and HT-29 cells. Digestion was performed according to the INFOGEST 2.0 protocol, adapted for elderly conditions (EC), including dialysis, to estimate absorbable fractions and calculate recovery (RR) and bioaccessibility index (BI). ELISA was used to evaluate IL-1β and TNF-α production in a Transwell system with inflamed epithelium. Results: The crude extract demonstrated 24.26 ± 0.84 mg EF/g TPC, 0.91 ± 0.05 mg EF/g PhT, and 17.67 ± 0.25 mg TE/g AOC, along with 45.5 g/100 of insoluble fiber. Safe concentrations were ≤1 g/100 mL for Caco-2 and ≤1.2 g/100 mL for HT-29. Following digestion, TPC and AOC increased significantly (p<0.05) by 190% and 71% respectively, indicating greater bioaccessibility in the elderly model vs adult model. PhT also increased (p<0.05) but demonstrated low bioaccessibility (~2%). IL-1β showed a temporary rise at 6 h, followed by a decline at 24 h, particularly in the crude extract (-45.2%) and elderly digestion (-24.7%). TNF-α appeared to decrease, achieving the most significant reduction at 12 h in the extract (-23.4%), though without significant differences. Conclusion: Simulated digestion improved the release and bioaccessibility of bioactive compounds, especially in the elderly model. The temporal modulation of IL-1β and TNF-α suggests a potential anti-inflammatory effect of the digested Durvillaea incurvata...