Browsing by Author "Spencer, Nicolás"
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Spencer, Nicolás; Avendano, Octavio; GARCIA, (1983)Mixed human rotavirus electropherotypes were detected in stool samples from patients with acute gastroenteritis in Santiago, Chile. These electropherotypes accounted for 10% of 149 samples studied. The finding of extra RNA ...
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Pizarro Pizarro, Daniel Iván; Sandino,; Pizarro Pizarro, Daniel Iván; Fernandez, Virginia; Spencer, Nicolás (1991)Rotaviruses transcribe mRNA containing a 7(m)GpppG(m)p cap at the 5' end in vitro. Guanylyltransferase activity associated with the viral particle was detected by SDS-PAGE due to the formation of a nucleotide-enzyme complex ...
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Spencer, Nicolás; GARCIA, (1984)The characteristics of human rotavirus-associated RNA polymerase activity have been examined in relation to the effects of ribonucleoside triphosphate analogs and S-adenosylmethionine. These effects were analyzed by testing ...
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Spencer, Nicolás; Araya,; Sandino,; Pacheco,; Brunser, (1988)Faecal excretion of enteropathogens was studied in newborns in their first week of life. Rotavirus was investigated in 225 neonates, of whom 107 belonged to the low socio-economic stratum (SES) and 118 to the high SES. ...
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Vasquez, Dayana; Sandino,; Pizarro Pizarro, Daniel Iván; Fernandez, Virginia; Valenzuela,; Spencer, Nicolás (1993)The phenotype of the rotavirus SA-11 mutant tsB carrying a thermosensitive mutation in gene 3, which encodes VP3, was characterized further from both infected cells and purified viral particles. The mutant phenotype was ...
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Carrasco,; González González, Larry Javier; Cumsille,; Concha, Luciana; de la Fuente,; Spencer, Nicolás; Verdejo, (1990)We analyzed causes of death before and after the March 1985 earthquake in Valparaíso, Chile. Causes of death were obtained from death certificates. Apart from death directly due to trauma during the earthquake, a significant ...
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Gaggero Brillouet, Aldo; Avendano, Octavio; Fernández, Virginia; Spencer, Nicolás (1992)We studied the transmission of rotavirus (RV) in 950 patients under 2 years of age hospitalized for diarrhea in Santiago, Chile. Stool samples were collected every other day from all patients during their entire hospital ...
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Photoaffinity labeling of rotavirus VP1 with 8-azido-ATP: Identification of the viral RNA polymerase Valenzuela,; Pizarro Pizarro, Daniel Iván; Sandino,; Vasquez, Dayana; Fernandez, Virginia; Hernandez,; Patton,; Spencer, Nicolás (1991)Rotavirus single-shelled particles have several enzymatic activities that are involved with the synthesis of capped mRNAs both in vivo and in vitro. Because single-shelled particles must be structurally intact to carry out ...
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Sandino,; Jashes,; Faundez,; Spencer, Nicolás (1986)The inner protein shell of human rotavirus consists of a single polypeptide called VP6 which was removed from the single-shelled virus by treatment with CaCl2, leaving the viral core. The core thus obtained was unable to ...
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Sandino,; Fernandez, Virginia; Pizarro Pizarro, Daniel Iván; Vasquez, Dayana; Spencer, Nicolás (1994)The structural relationship between VP6 (inner capsid polypeptide) and the viral core was studied using chemical cross-linking with dithiobis(succinimidyl propionate). Crosslinked single shelled and reconstituted rotavirus ...