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Authordc.contributor.authorSáenz Iturriaga, Leonardo Enrique 
Authordc.contributor.authorNeira Carrillo, Andrónico es_CL
Authordc.contributor.authorParedes, Rodolfo es_CL
Authordc.contributor.authorCortés, Marlies es_CL
Authordc.contributor.authorBucarey Vivanco, Sergio es_CL
Authordc.contributor.authorArias Bautista, José es_CL
Cita de ítemdc.identifier.citationINTERNATIONAL JOURNAL OF PHARMACEUTICS Volume: 369 Issue: 1-2 Pages: 64-71 Published: MAR 18 2009en_US
Identifierdc.identifier.otherDOI: 10.1016/j.ijpharm.2008.10.033
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractThree chitosan formulations were evaluated for their ability as adjuvant of a poor immunogenic peptide vaccine against GnRH-I. Male Sprague-Dawley rats were immunized subcutaneously with recombinant His-GnRH-tandem-repeat peptide in high. low and phosphorylated high molecular weight chitosan solution at 0.5% (w/v). Freund's complete adjuvant was used as a positive control of immune response. Our results suggest that different chitosan formulations as adjuvant, with high or low viscosity degree allow inducing a high and persistent immune response against a poor immunogenic recombinant peptide. We found that the immune response was mediated by a increasing of IgG isotype 1, which were significantly greater than levels presented by the animals immunized with Freund's complete adjuvant. Nevertheless, chitosan with low molecular weight and highest acetylation degree was able to induce an immune response mediated by IgG isotype 2a. Additionally, high molecular weight phosphorylated chitosan, in which the phosphate groups were linked to N-acetyl-D-glucosamine unit, the immune response was reduced. All the immune responses obtained with chitosan as adjuvant were able to neutralize effectively the GnRH hormone proves by reducing of animal steroidogenesis and spermatogenesis demonstrating its capacity to improve immunogenicity in peptide vaccine.en_US
Patrocinadordc.description.sponsorshipChilean Council for Science and Technology (CONICYT) PSD-23 University Andres Bello DI 44-06/R FONDAP 11980002en_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Títulodc.titleChitosan formulations improve the immunogenicity of a GnRH-I peptide-based vaccineen_US
Document typedc.typeArtículo de revistaen_US

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile